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Living organisms rely on molecular networks, such as gene circuits and signaling pathways, for information processing and robust decision-making in crowded, noisy environments. Recent advances show that interacting biomolecules…

Biomolecular condensates constitute a newly recognized form of spatial organization in living cells. Although many condensates are believed to form as a result of phase separation, the physicochemical properties that determine the phase…

Biological Physics · Physics 2023-07-11 William M. Jacobs

In living cells, protein-rich condensates can wet the cell membrane and surfaces of membrane-bound organelles. Interestingly, many phase-separating proteins also bind to membranes leading to a molecular layer of bound molecules. Here we…

Biological Physics · Physics 2022-03-22 Xueping Zhao , Giacomo Bartolucci , Alf Honigmann , Frank Jülicher , Christoph A. Weber

Biomolecular condensates play essential roles in cellular processes, and recent efforts have focused on understanding their assembly and rational design principles. In this study, we have employed an explicit-solvent minimal statistical…

Soft Condensed Matter · Physics 2026-05-26 Siddhartha Roy , Rakesh S. Singh

The physics and morphology of biomolecular condensates formed through liquid-liquid phase separation underpin diverse biological processes, exemplified by the nested organization of nucleoli that facilitates ribosome biogenesis. Here, we…

Biological Physics · Physics 2025-12-19 Sihan Liu , Andrej Košmrlj

Biomolecules, such as proteins and RNAs, can phase separate in the cytoplasm of cells to form biomolecular condensates. Such condensates are liquid-like droplets that can wet biological surfaces such as membranes. Many molecules that…

Soft Condensed Matter · Physics 2024-09-04 Xueping Zhao , Susanne Liese , Alf Honigmann , Frank Jülicher , Christoph A. Weber

Biomolecular condensates are formed via liquid-liquid phase separation of proteins, often together with nucleic acids, typically driven by interactions between low-affinity binding sites. The computational study of such condensates that…

Soft Condensed Matter · Physics 2025-01-16 Alena Taskina , Devika Magan , Simon Dannenberg , Stefan Klumpp

In living cells, proteins involved in specialized biochemical functions are often spatially organized within biomolecular condensates. Increasing evidence suggests that some of these condensates, including DNA repair condensates, emerge…

Soft Condensed Matter · Physics 2026-05-15 Léa Beaulès , Judith Miné-Hattab , Pierre Illien , Vincent Dahirel

This article focuses on phase and aggregation behavior for linear chains composed of blocks of hydrophilic and hydrophobic segments. Phase and conformational transitions of patterned chains are relevant for understanding liquid-liquid…

Soft Condensed Matter · Physics 2024-05-15 Athanassios Z. Panagiotopoulos

Biomolecular condensates play a crucial role in the spatial organization of living matter. These membrane-less organelles, resulting from liquid-liquid phase separation, operate far from thermodynamic equilibrium, with their size and…

Soft Condensed Matter · Physics 2025-05-12 Jacques Fries , Roxanne Berthin , Marie Jardat , Pierre Illien , Vincent Dahirel

Cells control the size and organization of biomolecular condensates formed by liquid-liquid phase separation (LLPS), but multiple mechanisms likely contribute to this control and remain to be fully elucidated. Here we propose a…

Biological Physics · Physics 2026-04-10 Le Qiao , Peter Gispert , Lukas S. Stelzl , Friederike Schmid

The separation of substances into different phases is ubiquitous in nature and important scientifically and technologically. This phenomenon may become drastically different if the species involved, whether molecules or supramolecular…

Biomolecular condensates have been shown to play a fundamental role in localizing biochemistry in a cell. RNA is a common constituent of condensates, and can determine their biophysical properties. Functions of biomolecular condensates are…

Subcellular Processes · Quantitative Biology 2023-02-22 Alex Mayer , Grace McLaughlin , Sierra Cole , Amy Gladfelter , Marcus Roper

Biomolecular condensates self-assemble when proteins and nucleic acids spontaneously demix to form droplets within the crowded intracellular milieu. This simple mechanism underlies the formation of a wide variety of membraneless…

Statistical Mechanics · Physics 2021-06-30 William M. Jacobs

Phase-separated biomolecular condensates exhibit a wide range of dynamical properties, which depend on the sequences of the constituent proteins and RNAs. However, it is unclear to what extent condensate dynamics can be tuned without also…

Biomolecules · Quantitative Biology 2024-07-31 Yaxin An , Michael A. Webb , William M. Jacobs

A diffuse-interface model for microstructure with an arbitrary number of components and phases was developed from basic thermodynamic and kinetic principles and formalized within a variational framework. The model includes a composition…

Materials Science · Physics 2011-07-28 Daniel A. Cogswell , W. Craig Carter

Biological mixtures such as the cytosol may consist of thousands of distinct components. There is now a substantial body of evidence showing that, under physiological conditions, intracellular mixtures can phase separate into spatially…

Soft Condensed Matter · Physics 2017-03-06 William M. Jacobs , Daan Frenkel

We perform simulations of a system containing simple model proteins and a polymer representing chromatin. We study the interplay between protein-protein and protein-chromatin interactions, and the resulting condensates which arise due to…

Soft Condensed Matter · Physics 2022-07-20 Marco Ancona , Chris A. Brackley

The interaction of phase-separating systems with chemical reactions is of great interest in various contexts, from biology to material science. In biology, phase separation is thought to be the driving force behind the formation of…

Soft Condensed Matter · Physics 2026-01-08 Franco Blanchini , Elisa Franco , Giulia Giordano , Dino Osmanovic

The self-organization of proteins into enriched compartments and the formation of complex patterns are crucial processes for life on the cellular level. Liquid-liquid phase separation is one mechanism for forming such enriched compartments.…

Soft Condensed Matter · Physics 2025-04-07 Antonia Winter , Yuhao Liu , Alexander Ziepke , George Dadunashvili , Erwin Frey
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