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Multiplexed imaging data are revolutionizing our understanding of the composition and organization of tissues and tumors. A critical aspect of such tissue profiling is quantifying the spatial relationship relationships among cells at…

Quantitative Methods · Quantitative Biology 2024-05-06 Ajit J. Nirmal , Peter K. Sorger

Single-cell RNA sequencing (scRNA-seq) provides high-dimensional profiles of cellular states, enabling data-driven modeling of cellular dynamics over time. In practice, time-resolved scRNA-seq is collected at only a few discrete time points…

Machine Learning · Computer Science 2026-05-22 Siyu Pu , Qingqing Long , Xiaohan Huang , Haotian Chen , Jiajia Wang , Meng Xiao , Xiao Luo , Hengshu Zhu , Yuanchun Zhou , Xuezhi Wang

The advancement of single-cell RNA-sequencing (scRNA-seq) technologies allow us to study the individual level cell-type-specific gene expression networks by direct inference of genes' conditional independence structures. scRNA-seq data…

Methodology · Statistics 2024-09-20 Changhao Ge , Hongzhe Li

Single-cell technologies have revolutionized biomedical research by enabling scalable measurement of the genome, transcriptome, and proteome of multiple systems at single-cell resolution. Now widely applied to cancer models, these assays…

Genomics · Quantitative Biology 2020-05-05 Allen W Zhang , Kieran R Campbell

Xenium, a new spatial transcriptomics platform, enables subcellular-resolution profiling of complex tumor tissues. Despite the rich morphological information in histology images, extracting robust cell-level features and integrating them…

Computer Vision and Pattern Recognition · Computer Science 2025-08-15 Paul H. Acosta , Pingjun Chen , Simon P. Castillo , Maria Esther Salvatierra , Yinyin Yuan , Xiaoxi Pan

Translating single-cell RNA sequencing (scRNA-seq) data into mechanistic biological hypotheses remains a critical bottleneck, as agentic AI systems lack direct access to transcriptomic representations while expression foundation models…

Genomics · Quantitative Biology 2026-03-18 Omar Coser

Single image super resolution (SR) has seen major performance leaps in recent years. However, existing methods do not allow exploring the infinitely many plausible reconstructions that might have given rise to the observed low-resolution…

Computer Vision and Pattern Recognition · Computer Science 2020-06-23 Yuval Bahat , Tomer Michaeli

Inferring gene regulatory networks (GRNs) from single-cell RNA sequencing (scRNA-seq) data is a complex challenge that requires capturing the intricate relationships between genes and their regulatory interactions. In this study, we tackle…

Machine Learning · Computer Science 2024-07-26 Sindhura Kommu , Yizhi Wang , Yue Wang , Xuan Wang

SMeagol is a software tool to simulate highly realistic microscopy data based on spatial systems biology models, in order to facilitate development, validation, and optimization of advanced analysis methods for live cell single molecule…

Biological Physics · Physics 2017-05-08 Martin Lindén , Vladimir Ćurić , Alexis Boucharin , David Fange , Johan Elf

We present SPUX - a modular framework for Bayesian inference enabling uncertainty quantification and propagation in linear and nonlinear, deterministic and stochastic models, and supporting Bayesian model selection. SPUX can be coupled to…

Computation · Statistics 2021-05-14 Jonas Šukys , Marco Bacci

RNA-sequencing has revolutionized biomedical research and, in particular, our ability to study gene alternative splicing. The problem has important implications for human health, as alternative splicing may be involved in malfunctions at…

Applications · Statistics 2015-12-11 David Rossell , Camille Stephan-Otto Attolini , Manuel Kroiss , Almond Stöcker

Cell populations are never truly homogeneous; individual cells exist in biochemical states that define functional differences between them. New technology based on microfluidic arrays combined with multiplexed quantitative polymerase chain…

Spatial transcriptomics (ST) is an emerging technology that enables researchers to investigate the molecular relationships underlying tissue morphology. However, acquiring ST data remains prohibitively expensive, and traditional fixed-grid…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Junchao Zhu , Ruining Deng , Junlin Guo , Tianyuan Yao , Chongyu Qu , Juming Xiong , Siqi Lu , Zhengyi Lu , Yanfan Zhu , Marilyn Lionts , Yuechen Yang , Yalin Zheng , Yu Wang , Shilin Zhao , Haichun Yang , Yuankai Huo

High-throughput RNA sequencing (RNA-seq) is now the standard method to determine differential gene expression. Identifying differentially expressed genes crucially depends on estimates of read count variability. These estimates are…

The oxDNA model of DNA has been applied widely to systems in biology, biophysics and nanotechnology. It is currently available via two independent open source packages. Here we present a set of clearly-documented exemplar simulations that…

Biomolecules · Quantitative Biology 2022-09-26 Aditya Sengar , Thomas E. Ouldridge , Oliver Henrich , Lorenzo Rovigatti , Petr Sulc

Urothelial cell carcinoma (UCC) is the ninth most common cancer that accounts for 4.7% of all the new cancer cases globally. UCC development and progression are due to complex and stochastic genetic programmes. To study the cascades of…

Genomic data has become increasingly accessible to the general public with the advent of companies offering whole genome sequencing at a relatively low cost. However, their reports are not verifiable due to a lack of crucial details and…

As the large amount of sequencing data accumulated in past decades and it is still accumulating, we need to handle the more and more sequencing data. As the fast development of the computing technologies, we now can handle a large amount of…

Other Quantitative Biology · Quantitative Biology 2021-01-05 Songting Shi

Publishing original scientific research is inherent to the work of a researcher. However, the pressure to maintain productivity and scientific impact can lead to research group publishing excessively, negatively affecting the mental health…

Computers and Society · Computer Science 2021-03-08 Sanjay Rathee , Sheah Lin Lee

In the era of data-driven science, conducting computational experiments that involve analysing large datasets using heterogeneous computational clusters, is part of the everyday routine for many scientists. Moreover, to ensure the…

Distributed, Parallel, and Cluster Computing · Computer Science 2022-03-23 Thanasis Vergoulis , Konstantinos Zagganas , Loukas Kavouras , Martin Reczko , Stelios Sartzetakis , Theodore Dalamagas