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Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

The outbreak of COVID-19 exposed the inadequacy of our technical tools for home health surveillance, and recent studies have shown the potential of smartphones as a universal optical microscopic imaging platform for such applications.…

Image and Video Processing · Electrical Eng. & Systems 2023-12-20 Haoran Zhang , Weiyi Zhang , Zirui Zuo , Jianlong Yang

High resolution optical endoscopes are increasingly used in diagnosis of various medical conditions of internal organs, such as the gastrointestinal tracts, but they are too expensive for use in resource-poor settings. On the other hand,…

Optics · Physics 2016-03-08 Xiangqian Hong , Vivek K. Nagarajan , Dale H. Mugler , Bing Yu

Optical sectioning technology has been widely used in various fluorescence microscopes owing to its background removing capability. Here, a virtual HiLo based on edge detection (V-HiLo-ED) is proposed to achieve wide-field optical…

Optics · Physics 2021-11-08 Yuyao Hu , Dong Liang , Jing Wang , Yaping Xuan , Fu Zhao , Jun Liu , Ruxin Li

Digital holography has applications in bio-imaging because it can simultaneously obtain the amplitude and phase information of a microscopic sample in a single shot, thus facilitating non-contact, noninvasive observation of the 3D shape of…

Graphics · Computer Science 2024-06-14 Yuki Nagahama

We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…

Instrumentation and Detectors · Physics 2011-06-17 Jan A. N. Buytaert , Emilie Descamps , Dominique Adriaens , Joris J. J. Dirckx

We propose to use high numerical aperture single mode optical fibers like photonic crystal fiber for lensless in-line holographic microscopy. Highly divergent beam helps to overcome the spatial sampling limitation of the image sensor. In…

Optics · Physics 2020-07-01 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab K. Dutta

Traditional silicon-on-insulator (SOI) platform based on-chip photonic interconnects have limited energy-bandwidth scalability due to the optical non-linearity induced power constraints of the constituent photonic devices. In this paper, we…

Applied Physics · Physics 2020-03-27 Venkata Sai Praneeth Karempudi , Sairam Sri Vatsavai , Ishan Thakkar

Expensive scientific camera hardware is amongst the main cost factors in modern, high-performance microscopes. Recent technological advantages have, however, yielded consumer-grade camera devices that can provide surprisingly good…

Image and Video Processing · Electrical Eng. & Systems 2019-03-06 Benedict Diederich , Patrick Then , Alexander Jügler , Ronny Förster , Rainer Heintzmann

Lensless imaging enables exceptionally compact fluorescence sensors, advancing applications in \textit{in vivo} imaging and low-cost, point-of-care diagnostics. These sensors require a filter to block the excitation light while passing…

Image and Video Processing · Electrical Eng. & Systems 2026-02-20 Lukas Harris , Micah Roschelle , Jack Bartley , Mekhail Anwar

A low cost fluorescence-based optical system is developed for detecting the presence of certain microorganisms and molecules within a diluted sample. A specifically designed device setup compatible with conventional 96 well plates is chosen…

Computer Vision and Pattern Recognition · Computer Science 2026-04-17 Zhendong Cao , Katrina G. Salvante , Ash Parameswaran , Pablo A. Nepomnaschy , Hongji Dai

Three-dimensional (3D) fluorescence imaging provides a vital approach for study of biological tissues with intricate structures, and optical sectioning structured illumination microscopy (OS-SIM) stands out for its high imaging speed, low…

Self-supervised Object Segmentation (SOS) aims to segment objects without any annotations. Under conditions of multi-camera inputs, the structural, textural and geometrical consistency among each view can be leveraged to achieve…

Computer Vision and Pattern Recognition · Computer Science 2025-01-20 Xiaoyun Zheng , Liwei Liao , Jianbo Jiao , Feng Gao , Ronggang Wang

Iris recognition is widely acknowledged for its exceptional accuracy in biometric authentication, traditionally relying on near-infrared (NIR) imaging. Recently, visible spectrum (VIS) imaging via accessible smartphone cameras has been…

Image and Video Processing · Electrical Eng. & Systems 2024-12-18 Naveenkumar G Venkataswamy , Yu Liu , Surendra Singh , Soumyabrata Dey , Stephanie Schuckers , Masudul H Imtiaz

The lensless endoscope (LE) is a promising device to acquire in vivo images at a cellular scale. The tiny size of the probe enables a deep exploration of the tissues. Lensless endoscopy with a multicore fiber (MCF) commonly uses a spatial…

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian

The difficulty of processing gigapixel whole slide images (WSIs) in clinical microscopy has been a long-standing barrier to implementing computer aided diagnostic systems. Since modern computing resources are unable to perform computations…

Computer Vision and Pattern Recognition · Computer Science 2020-08-13 Sam Maksoud , Kun Zhao , Peter Hobson , Anthony Jennings , Brian Lovell

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

Chip-based Evanescent Light Scattering (cELS) utilizes the multiple modes of a high-index contrast optical waveguide for near-field illumination of unlabeled samples, thereby repositioning the highest spatial frequencies of the sample into…

Mobile-phones have facilitated the creation of field-portable, cost-effective imaging and sensing technologies that approach laboratory-grade instrument performance. However, the optical imaging interfaces of mobile-phones are not designed…

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