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Sub-diffraction resolution, gentle sample illumination, and the possibility to image in multiple colors make Structured Illumination Microscopy (SIM) an imaging technique which is particularly well suited for live cell observations. Here,…

Among super-resolution microscopy techniques, structured illumination microscopy (SIM) shows great advances of low phototoxicity, high speed, and excellent performance in long-term dynamic observation, making it especially suitable for live…

Optics · Physics 2025-02-07 Han Wang , Wenshu Wang , Xinzhu Xu , Meiqi Li , Peng Xi

Structured illumination microscopy (SIM) enables live cell, super-resolution imaging at high speeds. SIM uses sophisticated optical systems to generate pre-determined excitation light patterns, and reconstruction algorithms to enhance the…

Structured Illumination Microscopy (SIM) overcomes the optical diffraction limit by folding high-frequency components into the baseband of the optical system, where they can be extracted and then repositioned to their original location in…

Optics · Physics 2024-11-18 Doron Shterman , Guy Bartal

Structured illumination microscopy (SIM) is a wide-field super-resolution technique normally limited to roughly twice the diffraction-limited resolution ($\approx 100$--$200$~nm). Surpassing this bound is a classic ill-posed inverse…

Structured illumination microscopy (SIM) provides images of fluorescent objects at an enhanced resolution greater than that of conventional epifluorescence wide-field microscopy. Initially demonstrated in 1999 to enhance the lateral…

Optics · Physics 2022-03-09 James D. Manton

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the…

Computer Vision and Pattern Recognition · Computer Science 2017-01-13 Li-Hao Yeh , Lei Tian , Laura Waller

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Structured illumination microscopy (SIM) is a very important super-resolution microscopy technique, which provides high speed super-resolution with about two-fold spatial resolution enhancement. Several attempts aimed at improving the…

Computer Vision and Pattern Recognition · Computer Science 2016-02-23 Amit Lal , Chunyan Shan , Peng Xi

Structured illumination microscopy (SIM) has become an important technique for optical super-resolution imaging because it allows a doubling of image resolution at speeds compatible for live-cell imaging. However, the reconstruction of SIM…

Image and Video Processing · Electrical Eng. & Systems 2020-03-26 Charles N. Christensen , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

Optics · Physics 2024-10-16 Doron Shterman , Guy Bartal

Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in…

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Structured illumination microscopy (SIM) has emerged as an essential technique for 3D and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the various aspects of SIM,…

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

Image and Video Processing · Electrical Eng. & Systems 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Intelligent surfaces represent a breakthrough technology capable of customizing the wireless channel cost-effectively. However, the existing works generally focus on planar wavefront, neglecting near-field spherical wavefront…

Information Theory · Computer Science 2025-02-11 Xing Jia , Jiancheng An , Hao Liu , Lu Gan , Marco Di Renzo , Mérouane Debbah , Chau Yuen

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

Optics · Physics 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

Super-resolution structured illumination microscopy (SR-SIM) is a widely used technique for enhancing the resolution of fluorescence imaging beyond the diffraction limit. Most existing SR-SIM methods rely on Moir\'e effect-based physical…

Optics · Physics 2025-02-27 Junzheng Peng , Jiahao Xian , Xi Lin , Manhong Yao , Shiping Li , Jingang Zhong
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