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Digital holography is one of the most widely used label-free microscopy techniques in biomedical imaging. Recovery of the missing phase information of a hologram is an important step in holographic image reconstruction. Here we demonstrate…

Image and Video Processing · Electrical Eng. & Systems 2021-05-28 Luzhe Huang , Tairan Liu , Xilin Yang , Yi Luo , Yair Rivenson , Aydogan Ozcan

Recovering ghost-free High Dynamic Range (HDR) images from multiple Low Dynamic Range (LDR) images becomes challenging when the LDR images exhibit saturation and significant motion. Recent Diffusion Models (DMs) have been introduced in HDR…

Computer Vision and Pattern Recognition · Computer Science 2024-04-02 Tao Hu , Qingsen Yan , Yuankai Qi , Yanning Zhang

One of the primary challenges in live cell culture observation is achieving high-contrast imaging with minimal impact on sample behavior. Quantitative phase imaging (QPI) techniques address this by providing label-free, high-contrast images…

Particle tracking is a fundamental task in digital microscopy. Recently, machine-learning approaches have made great strides in overcoming the limitations of more classical approaches. The training of state-of-the-art machine-learning…

We report a compact, cost-effective and field-portable lensless imaging platform for quantitative microscopy. In this platform, the object is placed on top of an image sensor chip without using any lens. We use a low-cost galvo scanner to…

Image and Video Processing · Electrical Eng. & Systems 2019-04-26 He Zhang , Zichao Bian , Shaowei Jiang , Jian Liu , Pengming Song , Guoan Zheng

We present a new technique for removing twin image in in-line digital Fourier holography using a combination of Fourier transformations. Instead of recording only a Fourier transform hologram of the object, we propose to record a combined…

Optics · Physics 2013-11-26 Debesh Choudhury , Gautam Lohar

Advances in endoscopy use in surgeries face challenges like inadequate lighting. Deep learning, notably the Denoising Diffusion Probabilistic Model (DDPM), holds promise for low-light image enhancement in the medical field. However, DDPMs…

Image and Video Processing · Electrical Eng. & Systems 2024-05-20 Tong Chen , Qingcheng Lyu , Long Bai , Erjian Guo , Huxin Gao , Xiaoxiao Yang , Hongliang Ren , Luping Zhou

We have developed a hyperspectral imaging scheme that involves a combination of dual-comb spectroscopy and Hadamard-transform-based single-pixel imaging. The scheme enables us to obtain 12,000 hyperspectral images of amplitude and phase at…

Scanning laser confocal holographic microscopy using a spatial heterodyne detection method is presented. Spatial heterodyne detection technique employs a Mach-Zehnder interferometer with the reference beam frequency shifted by two…

Instrumentation and Detectors · Physics 2016-10-14 Changgeng Liu

Quantitative phase microscopy (QPM) enables label-free measurement of local optical path length variations, providing critical insight into the structure and dynamics of transparent biological specimens. Here, a highly sensitive lateral…

Mapping a single exposure low dynamic range (LDR) image into a high dynamic range (HDR) is considered among the most strenuous image to image translation tasks due to exposure-related missing information. This study tackles the challenges…

Computer Vision and Pattern Recognition · Computer Science 2021-04-20 SMA Sharif , Rizwan Ali Naqvi , Mithun Biswas , Kim Sungjun

We report the modification of a label-free image scanning microscope (ISM) to perform asynchronous 2D imaging at 24kHz while keeping the lateral resolution gain and background rejection of a regular label-free ISM setup. Our method uses a…

Optics · Physics 2023-08-31 Duc-Minh Ta , Alberto Aguilar , Pierre Bon

Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of…

Optics · Physics 2020-11-04 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Unified image restoration is a significantly challenging task in low-level vision. Existing methods either make tailored designs for specific tasks, limiting their generalizability across various types of degradation, or rely on training…

Computer Vision and Pattern Recognition · Computer Science 2026-03-10 Huaqiu Li , Yong Wang , Tongwen Huang , Hailang Huang , Haoqian Wang , Xiangxiang Chu

Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather…

Optics · Physics 2020-10-23 Wonjun Choi , Munkyu Kang , Jin Hee Hong , Ori Katz , Youngwoon Choi , Wonshik Choi

A lateral shear interferometer based digital holographic microscopy has been realized to study the morphology dynamics of Human red blood cells quantitatively. Here, a lateral shear interferometer is embedded with a conventional microscope…

Image and Video Processing · Electrical Eng. & Systems 2019-09-10 Shital Devinder , Ashish Lal , Tathagato Rai Dastidar , Satish Kumar Dubey

Non-invasive and single-shot holographic imaging through complex media is technically challenging due to random light scattering which significantly scrambles optical information. Recently, several methods have been presented to address…

A lensfree spectral light-field fusion microscopy (LSLFM) system is presented for enabling contrast- and resolution-enhanced imaging of biological specimens. LSLFM consists of a pulsed multispectral lensfree microscope for capturing…

In-line digital holography (DIH) is a widely used lensless imaging technique, valued for its simplicity and capability to image samples at high throughput. However, capturing only intensity of the interference pattern during the recording…

Image and Video Processing · Electrical Eng. & Systems 2026-03-06 Gökhan Koçmarlı , G. Bora Esmer

Optical endoscopy plays a crucial role in minimally invasive medical diagnostics and therapeutic procedures. Nonetheless, state-of-the-art endoscopic zoom objectives are bulky, requiring multi-element lens actuation, which can limit…