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Image segmentation is a fundamental problem in biomedical image analysis. Recent advances in deep learning have achieved promising results on many biomedical image segmentation benchmarks. However, due to large variations in biomedical…

Computer Vision and Pattern Recognition · Computer Science 2017-06-16 Lin Yang , Yizhe Zhang , Jianxu Chen , Siyuan Zhang , Danny Z. Chen

Deep learning has revolutionized medical and biological imaging, particularly in segmentation tasks. However, segmenting biological cells remains challenging due to the high variability and complexity of cell shapes. Addressing this…

Computer Vision and Pattern Recognition · Computer Science 2025-06-03 Tianhao Zhang , Heather J. McCourty , Berardo M. Sanchez-Tafolla , Anton Nikolaev , Lyudmila S. Mihaylova

Microscopy images from different imaging conditions, organs, and tissues often have numerous cells with various shapes on a range of backgrounds. As a result, designing a deep learning model to count cells in a source domain becomes…

Computer Vision and Pattern Recognition · Computer Science 2023-03-21 Zuhui Wang

While recent advances in deep learning have significantly advanced the state of the art for vessel detection in color fundus (CF) images, the success for detecting vessels in fluorescein angiography (FA) has been stymied due to the lack of…

Computer Vision and Pattern Recognition · Computer Science 2020-05-12 Li Ding , Mohammad H. Bawany , Ajay E. Kuriyan , Rajeev S. Ramchandran , Charles C. Wykoff , Gaurav Sharma

The proliferation of digital microscopy images, driven by advances in automated whole slide scanning, presents significant opportunities for biomedical research and clinical diagnostics. However, accurately annotating densely packed…

Computer Vision and Pattern Recognition · Computer Science 2025-04-25 Chen Liu , Danqi Liao , Alejandro Parada-Mayorga , Alejandro Ribeiro , Marcello DiStasio , Smita Krishnaswamy

Recently deep residual learning with residual units for training very deep neural networks advanced the state-of-the-art performance on 2D image recognition tasks, e.g., object detection and segmentation. However, how to fully leverage…

Computer Vision and Pattern Recognition · Computer Science 2016-08-23 Hao Chen , Qi Dou , Lequan Yu , Pheng-Ann Heng

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

Though performed almost effortlessly by humans, segmenting 2D gray-scale or color images into respective regions of interest (e.g.~background, objects, or portions of objects) constitutes one of the greatest challenges in science and…

Computer Vision and Pattern Recognition · Computer Science 2023-08-23 Alexandre Benatti , Luciano da F. Costa

This paper presents a new deep regression model, which we call DeepDistance, for cell detection in images acquired with inverted microscopy. This model considers cell detection as a task of finding most probable locations that suggest cell…

Computer Vision and Pattern Recognition · Computer Science 2019-08-30 Can Fahrettin Koyuncu , Gozde Nur Gunesli , Rengul Cetin-Atalay , Cigdem Gunduz-Demir

Three-dimensional (3D) fluorescence microscopy in general requires axial scanning to capture images of a sample at different planes. Here we demonstrate that a deep convolutional neural network can be trained to virtually refocus a 2D…

Computer Vision and Pattern Recognition · Computer Science 2019-11-05 Yichen Wu , Yair Rivenson , Hongda Wang , Yilin Luo , Eyal Ben-David , Laurent A. Bentolila , Christian Pritz , Aydogan Ozcan

Object segmentation and structure localization are important steps in automated image analysis pipelines for microscopy images. We present a convolution neural network (CNN) based deep learning architecture for segmentation of objects in…

Computer Vision and Pattern Recognition · Computer Science 2019-01-24 Shan E Ahmed Raza , Linda Cheung , Muhammad Shaban , Simon Graham , David Epstein , Stella Pelengaris , Michael Khan , Nasir M. Rajpoot

Live-cell imaging of multiple subcellular structures is essential for understanding subcellular dynamics. However, the conventional multi-color sequential fluorescence microscopy suffers from significant imaging delays and limited number of…

Subcellular Processes · Quantitative Biology 2025-01-13 Mingyang Chen , Luhong Jin , Xuwei Xuan , Defu Yang , Yun Cheng , Ju Zhang

Fluorescence microscopy has enabled a dramatic development in modern biology. Due to its inherently weak signal, fluorescence microscopy is not only much noisier than photography, but also presented with Poisson-Gaussian noise where Poisson…

Computer Vision and Pattern Recognition · Computer Science 2019-04-09 Yide Zhang , Yinhao Zhu , Evan Nichols , Qingfei Wang , Siyuan Zhang , Cody Smith , Scott Howard

Fluorescence microscopy is a key driver to promote discoveries of biomedical research. However, with the limitation of microscope hardware and characteristics of the observed samples, the fluorescence microscopy images are susceptible to…

Image and Video Processing · Electrical Eng. & Systems 2022-09-15 Xuanyu Tian , Qing Wu , Hongjiang Wei , Yuyao Zhang

The glow of a starry night sky, the smell of a freshly brewed cup of coffee or the sound of ocean waves breaking on the beach are representations of the physical world that have been created by the dynamic interactions of thousands of…

Neurons and Cognition · Quantitative Biology 2011-07-28 Henry Lütcke , Fritjof Helmchen

Volumetric imaging of samples using fluorescence microscopy plays an important role in various fields including physical, medical and life sciences. Here we report a deep learning-based volumetric image inference framework that uses 2D…

Optics · Physics 2021-03-24 Luzhe Huang , Yilin Luo , Yair Rivenson , Aydogan Ozcan

Deep neural networks (DNNs) have demonstrated exceptional performance across various image segmentation tasks. However, the process of preparing datasets for training segmentation DNNs is both labor-intensive and costly, as it typically…

Computer Vision and Pattern Recognition · Computer Science 2025-01-24 Yixin Zhang , Shen Zhao , Hanxue Gu , Maciej A. Mazurowski

Automated cell segmentation in microscopy images is essential for biomedical research, yet conventional methods are labor-intensive and prone to error. While deep learning-based approaches have proven effective, they often require large…

Computer Vision and Pattern Recognition · Computer Science 2024-11-20 Rüveyda Yilmaz , Kaan Keven , Yuli Wu , Johannes Stegmaier

Image annotation aims to annotate a given image with a variable number of class labels corresponding to diverse visual concepts. In this paper, we address two main issues in large-scale image annotation: 1) how to learn a rich feature…

Computer Vision and Pattern Recognition · Computer Science 2018-10-22 Yulei Niu , Zhiwu Lu , Ji-Rong Wen , Tao Xiang , Shih-Fu Chang

Cell instance segmentation in fluorescence microscopy images is becoming essential for cancer dynamics and prognosis. Data extracted from cancer dynamics allows to understand and accurately model different metabolic processes such as…