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Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…

We propose a chiral imaging modality based on optical chirality engineering, fluorescence-detected circular dichroism and structured illumination microscopy. In this method, the optical chirality of the illumination is structured and the…

The use of fluorescent molecules to create long sequences of low-density, diffraction-limited images enables highly-precise molecule localization. However, this methodology requires lengthy imaging times, which limits the ability to view…

Image and Video Processing · Electrical Eng. & Systems 2024-03-26 Yair Ben Sahel , Yonina C. Eldar

A novel method for fast information retrieval from a probe storage device is considered. It is shown that information can be stored and retrieved using the optical diffraction patterns obtained by the illumination of a large array of…

Information Theory · Computer Science 2015-05-27 Joost van Honschoten , Henri de Jong , Wabe W. Koelmans , Thomas P. Parnell , Oleg V. Zaboronski

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by…

Quantitative Methods · Quantitative Biology 2023-08-09 Afonso Mendes , Hannah S. Heil , Simao Coelho , Christophe Leterrier , Ricardo Henriques

An approach reported recently by Alexandrov et al. on optical scatter imaging, termed digital Fourier microscopy (DFM), represents an adaptation of digital Fourier holography to selective imaging of biological matter. Holographic mode of…

Optics · Physics 2007-05-23 K. Y. T. Seet , P. Blazkiewicz , P. Meredith , A. V. Zvyagin

We present a theoretical justification for a method of extracting of supplementary information for the phase retrieval procedure taken from diffraction of fs-pulses from X-ray Free Electron Laser facilities. The approach is based on…

Materials Science · Physics 2015-02-18 A. Leonov , D. Ksenzov , A. Benediktovitch , I. Feranchuk , U. Pietsch

Structured illumination microscopy (SIM) is an important super-resolution based microscopy technique that breaks the diffraction limit and enhances optical microscopy systems. With the development of biology and medical engineering, there…

Image and Video Processing · Electrical Eng. & Systems 2021-11-18 Xi Cheng , Jun Li , Qiang Dai , Zhenyong Fu , Jian Yang

We report on a novel bichromatic fluorescent imaging scheme for background-free detection of single CaF molecules trapped in an optical tweezer array. By collecting fluorescence on one optical transition while using another for…

Atomic Physics · Physics 2024-06-18 Connor M. Holland , Yukai Lu , Lawrence W. Cheuk

Novel optical elements containing semitransparent wavelike films embedded into the bulk of transparent material, which form a reflection image without transmitted light distortion, are studied. The dynamic theory of light diffraction by a…

Optics · Physics 2018-05-23 Anatoly M. Smolovich , Valery Chernov

Unprecedented atomic-scale measurement resolution has recently been demonstrated in single-shot optical localization metrology based on deep-learning analyses of diffraction patterns of topologically structured light scattered from objects.…

When inverting solar spectra, image degradation effects that are present in the data are usually approximated or not considered. We develop a data reduction method that takes these issues into account and minimizes the resulting errors. By…

Instrumentation and Methods for Astrophysics · Physics 2015-06-11 M. van Noort

Understanding how networks of neurons process information is one of the key challenges in modern neuroscience. A necessary step to achieve this goal is to be able to observe the dynamics of large populations of neurons over a large area of…

Image and Video Processing · Electrical Eng. & Systems 2022-03-09 Pingfan Song , Herman Verinaz Jadan , Carmel L. Howe , Amanda J. Foust , Pier Luigi Dragotti

Diffraction tomography is a widely used inverse scattering technique for quantitative imaging of weakly scattering media. In its conventional formulation, diffraction tomography assumes monochromatic plane wave illumination. This…

Numerical Analysis · Mathematics 2026-03-11 Peter Elbau , Noemi Naujoks , Otmar Scherzer

We present an imaging technique that allows the recovery of the transparency profile of wavelength-scale objects with deep subwavelength resolution based on far-field intensity measurements. The approach, interscale mixing microscopy (IMM),…

Optics · Physics 2017-03-21 Sandeep Inampudi , Nicholas Kuhta , Viktor A. Podolskiy

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

Super-resolution imaging methods that combine interferometric (z) analysis with single-molecule localization microscopy (iSMLM) have achieved ultra-high 3D precision and contributed to the elucidation of important biological…

Optics · Physics 2025-02-20 Wei Wang , Zengxin Huang , Yilin Wang , Hangfeng Li , Pakorn Kanchanawong

Deep learning has become an extremely effective tool for image classification and image restoration problems. Here, we apply deep learning to microscopy, and demonstrate how neural networks can exploit the chromatic dependence of the…

Optics · Physics 2018-07-05 Eran Hershko* , Lucien E. Weiss* , Tomer Michaeli , Yoav Shechtman

Light field cameras capture multi-view observations within a single exposure. However, existing studies are typically tailored to specific LF representations, leaving the field without a unified learning framework. To bridge this gap, we…

Computer Vision and Pattern Recognition · Computer Science 2026-05-22 Fei Teng , Lingxin Huang , Buyin Deng , Kai Luo , Boyuan Zheng , Zheng Fang , Hong Zheng , Kunyu Peng , Jiaming Zhang , Yaonan Wang , Kailun Yang

In this technical review we give an introduction to optical spectroscopy for layered materials as a powerful, non-invasive tool to access details of the electronic band structure and crystal quality. Potential applications in photonics and…

Materials Science · Physics 2020-12-18 Shivangi Shree , Ioannis Paradisanos , Xavier Marie , Cedric Robert , Bernhard Urbaszek