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Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

Cryo-electron microscopy (cryo-EM) is a powerful technique in structural biology and drug discovery, enabling the study of biomolecules at high resolution. Significant advancements by structural biologists using cryo-EM have led to the…

Biomolecules · Quantitative Biology 2024-12-05 Yi Zhou , Yilai Li , Jing Yuan , Quanquan Gu

High-resolution structural information on membrane proteins is essential for understanding cell biology and for structure-based design of new medical drugs and drug delivery strategies. X-ray diffraction (XRD) can provide {\AA}ngstrom-level…

Biological Physics · Physics 2019-10-29 L. Schmüser , M. Trefz , S. J. Roeters , W. Beckner , J. Pfaendtner , D. Otzen , S. Woutersen , M. Bonn , D. Schneider , T. Weidner

The quartz crystal microbalance (QCM) is widely used to study surface adsorbed molecules, often of biological significance. However, the relation between raw acoustic response (frequency shift $\Delta f$ and dissipation factor $\Delta D$)…

Experimental X-ray crystallography, NMR (Nuclear Magnetic Resonance) spectroscopy, dual polarization interferometry, etc are indeed very powerful tools to determine the 3-Dimensional structure of a protein (including the membrane protein);…

Optimization and Control · Mathematics 2013-01-03 Jiapu Zhang , Yating Hou , Yiju Wang , Changyu Wang , Xiangsun Zhang

X-ray absorption spectroscopy (XAS) is a premier technique for materials characterization, providing key information about the local chemical environment of the absorber atom. In this work, we develop a database of sulfur K-edge XAS spectra…

We propose a novel approach to detect the binding between proteins making use of the anomalous diffraction of natively present heavy elements inside the molecule 3D structure. In particular, we suggest considering sulfur atoms contained in…

The OH-terminated self-assembled monolayers, as protein-resistant surfaces, have significant potential in biocompatible implant devices, which can avoid or reduce adverse reactions caused by protein adhesion to biomaterial surfaces, such as…

Applied Physics · Physics 2022-12-08 Dangxin Mao , Yuan-Yan Wu , Yusong Tu

Our study aims at a better control and understanding of the transfer of a complex [DNA supercoiled plasmid - dodecyltrimethylammonium surfactant] layer from a liquid-vapour water interface onto a silicon surface without any additional…

Chemical Physics · Physics 2008-01-18 Frédérique Battin-Leclerc , A. A. Konnov , J. L. Jaffrezo , M. Legrand

We present a detailed study of the interface morphology of an electro-deposited (ED) Ni/Cu bilayer film by using off-specular (diffuse) neutron reflectivity technique and Atomic Force Microscopy (AFM). The Ni/Cu bilayer has been…

Materials Science · Physics 2015-05-13 Surendra Singh , Saibal Basu , S. K. Ghosh

Multilayers of hydrogenated ultrathin (3 nm) amorphous a-Si and a-Ge layers prepared by sputtering have been studied by atomic force microscopy (AFM) and transmission electron microscopy (TEM) to check the influence of annealing on their…

Materials Science · Physics 2009-11-13 C. Frigeri , L. Nasi , M. Serenyi , A. Csik , Z. Erdelyi , D. L. Beke

Our current understanding of the structure and dynamics of aqueous interfaces at the molecular level has grown substantially in the last few decades due to the continuous development of surface-specific spectroscopies, such as vibrational…

Chemical Physics · Physics 2023-09-27 Yair Litman , Jinggang Lan , Yuki Nagata , David M. Wilkins

Computational modelling offers a cost-effective and time-efficient alternative to experimental studies in biomedical engineering. In cardiac electro-mechanics, finite element method (FEM)-based simulations provide valuable insights into…

Computational Engineering, Finance, and Science · Computer Science 2025-08-20 Tan Tran , Denisa Martonova , Sigrid Leyendecker

Small-angle X-ray scattering (SAXS) technique enables convenient nanoscopic characterization for various systems and conditions. Nonetheless, lab-based SAXS systems intrinsically suffer from insufficient x-ray flux and limited angular…

Instrumentation and Detectors · Physics 2020-09-30 Benjamin Gutman , Michael Mrejen , Gil Shabat , Ram Avinery , Yoel Shkolnisky , Roy Beck

The interaction of a protein with its environment can be understood and controlled via its 3D structure. Experimental methods for protein structure determination, such as X-ray crystallography or cryogenic electron microscopy, shed light on…

Machine Learning · Computer Science 2025-04-24 Axel Levy , Eric R. Chan , Sara Fridovich-Keil , Frédéric Poitevin , Ellen D. Zhong , Gordon Wetzstein

Multi-phase vapor-deposited glasses are an important class of materials for organic electronics, particularly organic photovoltaics and thermoelectrics. These blends are frequently regarded as molecular alloys and there have been few…

Identifying pathogens in complex samples such as blood, urine, and wastewater is critical to detect infection and inform optimal treatment. Surface-enhanced Raman spectroscopy (SERS) and machine learning (ML) can distinguish among multiple…

Craniofacial Superimposition is a forensic technique for identifying skeletal remains by comparing a post-mortem skull with ante-mortem facial photographs. A critical step in this process is Skull-Face Overlay (SFO). This stage involves…

Computer Vision and Pattern Recognition · Computer Science 2026-03-18 Práxedes Martínez-Moreno , Andrea Valsecchi , Pablo Mesejo , Pilar Navarro-Ramírez , Valentino Lugli , Sergio Damas

Label-free biosensors, including conventional quartz-crystal-microbalance (QCM) biosensor, are seriously affected by nonspecific adsorption of contaminants involved in analyte solution, and it is exceptionally difficult to extract the…

Biological Physics · Physics 2021-12-15 Kentaro Noi , Arihiro Iwata , Fumihito Kato , Hirotsugu Ogi

A novel method for measuring the surface coverage of randomly distributed cylindrical nanoparticles such as nanorods and nanowires, using atomic force microscopy (AFM), is presented. The method offers several advantages over existing…

Materials Science · Physics 2018-07-12 Francesca Bottacchi , Stefano Bottacchi , Thomas D. Anthopoulos