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Patient-specific therapies require that cells be manufactured in multiple batches of small volumes, making it a challenge for conventional modes of quality control. The added complexity of inherent variability (even within batches)…

Biological Physics · Physics 2018-04-17 Derrick Yong , Ahmad Amirul Abdul Rahim , Jesslyn Ong , May Win Naing

Label-free single-cell imaging offers a scalable, non-invasive alternative to fluorescence-based cytometry, yet inferring molecular phenotypes directly from bright-field morphology remains challenging. We present a unified Deep Learning…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Saqib Nazir , Ardhendu Behera

We present a reliable and robust integrated fluorescence detector capable of detecting single atoms. The detector consists of a tapered lensed single-mode fiber for precise delivery of excitation light and a multimode fiber to collect the…

Single molecule F\"orster resonance energy transfer (smFRET) is widely used to monitor conformations and interactions dynamics at the molecular level. However, conventional smFRET measurements are ineffective at donor-acceptor distances…

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

Single particle diffraction imaging experiments at free-electron lasers (FEL) have a great potential for structure determination of reproducible biological specimens that can not be crystallized. One of the challenges in processing the data…

Biological Physics · Physics 2013-10-16 O. M. Yefanov , I. A. Vartanyants

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…

Novel techniques are currently being developed and established for the accurate chemical analysis and detection of single cancer cells, single embryos and single seeds by Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fourier…

Tissues and Organs · Quantitative Biology 2007-05-23 I. C. Baianu , D. Costescu , N. E. Hofmann , S. S. Korban

This communication reports the effect of DNA conformation on fluorescence resonance energy transfer (FRET) efficiency between two laser dyes in layer by layer (LbL) self assembled film. The dyes Acraflavine and Rhodamine B were attached…

Scalable atom-based quantum platforms for simulation, computing, and metrology require fast high-fidelity, low-loss imaging of individual atoms. Standard fluorescence detection methods rely on continuous cooling, limiting the detection…

The analysis of entangled atomic ensembles and their application for interferometry beyond the standard quantum limit requires an accurate determination of the number of atoms. We present an accurate fluorescence detection technique for…

Quantum Physics · Physics 2019-12-13 A. Hüper , C. Pür , M. Hetzel , J. Geng , J. Peise , I. Kruse , M. Kristensen , W. Ertmer , J. Arlt , C. Klempt

Even with the luxury of having abundant data, multi-label classification is widely known to be a challenging task to address. This work targets the problem of multi-label meta-learning, where a model learns to predict multiple labels within…

Computer Vision and Pattern Recognition · Computer Science 2021-10-27 Christian Simon , Piotr Koniusz , Mehrtash Harandi

Aberrations and multiple scattering in biological tissues critically distort light beams into highly complex speckle patterns. In this regard, digital optical phase conjugation (DOPC) is a promising technique enabling in-depth focusing.…

Resonant energy transfers, i.e. the non-radiative redistribution of an electronic excitation between two particles coupled by the dipole-dipole interaction, lie at the heart of a variety of chemical and biological phenomena, most notably…

The applications of Fluorescence resonance energy transfer (FRET) have expanded tremendously in the last 25 years, and the technique has become a staple technique in many biological and biophysical fields. FRET can be used as spectroscopic…

Semi monolithic detector designs with a series of stacked thin monolithic scintillator plates and side readout are an attractive approach for potentially achieving very high performance in a positron emission tomography (PET) scanner. In…

Instrumentation and Detectors · Physics 2025-02-12 Shiv K. Subedi , Simon R. Cherry , Yi Qiang , Peng Peng

One of the most interesting predicted applications of graphene monolayer based devices is as high quality sensors. In this letter we show, through systematic experiments, a chemical vapor sensor based on the measurement of low frequency…

Mesoscale and Nanoscale Physics · Physics 2015-11-20 Kazi Rafsanjani Amin , Aveek Bid

Ensemble averaging experiments may conceal many fundamental molecular interactions. To overcome that, a high-throughput detection of single molecules or colloidal nanodots is crucial for biomedical, nanoelectronic, and solid-state…

Biological Physics · Physics 2019-07-15 Siddharth Ghosh , Narain Karedla , Ingo Gregor

The rapid advancement of diffusion models, particularly Stable Diffusion 3.5, has enabled the generation of highly photorealistic synthetic images that pose significant challenges to existing detection methods. This paper presents…

Computer Vision and Pattern Recognition · Computer Science 2025-07-09 Guang Yang

Zero-mode waveguide (ZMW) nanoapertures are widely used to monitor single molecules beyond the range accessible to normal microscopes. However, several aspects of the ZMW influence on the photophysics of fluorophores remain inadequately…

Optics · Physics 2022-05-04 Satyajit Patra , Jean-Benoît Claude , Jérôme Wenger