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Related papers: Loops are Geometric Catalysts for DNA Integration

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In understanding and redesigning the function of proteins in modern biochemistry, protein engineers are increasingly focusing on exploring regions in proteins called loops. Analyzing various characteristics of these regions helps the…

We use a recently developed coarse-grained model for DNA to study kissing complexes formed by hybridization of complementary hairpin loops. The binding of the loops is topologically constrained because their linking number must remain…

Soft Condensed Matter · Physics 2012-12-24 Flavio Romano , Alex Hudson , Jonathan P. K. Doye , Thomas E. Ouldridge , Ard A. Louis

It is well known that the structural deformations (stressed states) of DNA molecule play a crucial role in its biological functions including gene expression. For instance, looping in DNA (often mediated by protein binding) is a crucial…

Biological Physics · Physics 2007-05-23 Sachin Goyal , Noel C. Perkins

Motivated by the wide ranging experimental results on the conductivity of DNA, we have investigated extraordinary configurations and chemical environments in which DNA might become a true molecular wire, perticularly from enhanced…

Soft Condensed Matter · Physics 2009-09-29 R. G. Endres , D. L. Cox , R. R. P. Singh

It is generally difficult to predict the positions of mutations in genomic DNA at the nucleotide level. Retroviral DNA insertion is one mode of mutation, resulting in host infections that are difficult to treat. This mutation process…

Biological Physics · Physics 2017-07-04 Tatsuaki Tsuruyama

DNA is subject to large deformations in a wide range of biological processes. Two key examples illustrate how such deformations influence the readout of the genetic information: the sequestering of eukaryotic genes by nucleosomes, and DNA…

Biomolecules · Quantitative Biology 2015-11-10 Stephanie Johnson , Martin Lindén , Rob Phillips

We show the existence of a high interrelation between the different loops that may appear in a DNA segment. Conformational changes in a chain segment caused by the formation of a particular loop may either promote or prevent the appearance…

Biomolecules · Quantitative Biology 2009-03-24 Artur Garcia-Saez , J. Miguel Rubi

The mechanical properties of DNA play a critical role in many biological functions. For example, DNA packing in viruses involves confining the viral genome in a volume (the viral capsid) with dimensions that are comparable to the DNA…

Living cells provide a fluctuating, out-of-equilibrium environment in which genes must coordinate cellular function. DNA looping, which is a common means of regulating transcription, is very much a stochastic process; the loops arise from…

Biomolecules · Quantitative Biology 2015-05-18 Yih-Fan Chen , J. N. Milstein , Jens-Christian Meiners

We use a statistical mechanical model to study nonthermal denaturation of DNA in the presence of protein-mediated loops. We find that looping proteins which randomly link DNA bases located at a distance along the chain could cause a…

Soft Condensed Matter · Physics 2009-12-22 K. G. Petrosyan , Chin-Kun Hu

The classic model of eukaryotic gene expression requires direct spatial contact between a distal enhancer and a proximal promoter. Recent Chromosome Conformation Capture (3C) studies show that enhancers and promoters are embedded in a…

Genomics · Quantitative Biology 2014-11-25 Boryana Doyle , Geoffrey Fudenberg , Maxim Imakaev , Leonid A. Mirny

Microscopic mechanisms of positive charge transfer in DNA remain unclear. A quantum state of electron hole in DNA is determined by the competition of a pi-stacking interaction $b$ smearing a charge between different base pairs and…

Soft Condensed Matter · Physics 2008-03-15 D. B. Uskov , A. L. Burin

All known life forms are based upon a hierarchy of interwoven feedback loops, operating over a cascade of space, time and energy scales. Among the most basic loops are those connecting DNA and proteins. For example, in genetic networks, DNA…

Other Quantitative Biology · Quantitative Biology 2018-04-13 Tsvi Tlusty

The mechanism behind mutual recognition of homologous DNA sequences prior to genetic recombination is one of the remaining puzzles in molecular biology. Leading models of homology recognition, based on classical electrostatics, neglect the…

Biological Physics · Physics 2024-08-26 Ehud Haimov , Jonathan G. Hedley , Alexei A. Kornyshev

Loop extrusion convincingly describes how certain Structural Maintenance of Chromosome (SMC) proteins mediate the formation of large DNA loops. Yet, most of the existing computational models cannot reconcile recent in vitro observations…

Soft Condensed Matter · Physics 2022-01-12 Andrea Bonato , Davide Michieletto

Positioning of nucleosomes along eukaryotic genomes plays an important role in their organization and regulation. There are many different factors affecting the location of nucleosomes. Some can be viewed as preferential binding of a single…

Biological Physics · Physics 2015-08-13 Michael Sheinman , Ho-Ryun Chung

Chromatin is a complex of DNA, RNA and proteins whose primary function is to package genomic DNA into the tight confines of a cell nucleus. A fundamental repeating unit of chromatin is the nucleosome, an octamer of histone proteins around…

Genomics · Quantitative Biology 2012-06-21 Denis Tolkunov , Alexandre V. Morozov

The effect of sequence heterogeneity on polynucleotide translocation across a pore and on simple models of molecular motors such as helicases, DNA polymerase/exonuclease and RNA polymerase is studied in detail. Pore translocation of RNA or…

Statistical Mechanics · Physics 2009-11-10 Yariv Kafri , David K. Lubensky , David R. Nelson

In many cases, transcriptional regulation involves the binding of transcription factors at sites on the DNA that are not immediately adjacent to the promoter of interest. This action at a distance is often mediated by the formation of DNA…

Biomolecules · Quantitative Biology 2015-05-13 Lin Han , Hernan G. Garcia , Seth Blumberg , Kevin B. Towles , John F. Beausang , Philip C. Nelson , Rob Phillips

Chromosomes are crumpled polymer chains further folded into a sequence of stochastic loops via loop extrusion. While extrusion has been verified experimentally, the particular means by which the extruding complexes bind DNA polymer remains…

Soft Condensed Matter · Physics 2023-06-14 Kirill Polovnikov , Bogdan Slavov