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In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

Point-spread function (PSF) estimation in spatially undersampled images is challenging because large pixels average fine-scale spatial information. This is problematic when fine-resolution details are necessary, as in optimal photometry…

Instrumentation and Methods for Astrophysics · Physics 2021-02-03 Teresa Symons , Michael Zemcov , James Bock , Yun-Ting Cheng , Brendan Crill , Christopher Hirata , Stephanie Venuto

Point-spread function of the probe forming optics ($PSF_{optics} $) is reported for the first time in an uncorrected (without multipole correctors) scanning electron microscope (SEM). In an SEM, the electron probe information is lost as the…

Materials Science · Physics 2024-07-02 Surya Kamal , Richard K. Hailstone

Modeling the Point Spread Function (PSF) of wide-field surveys is vital for many astrophysical applications and cosmological probes including weak gravitational lensing. The PSF smears the image of any recorded object and therefore needs to…

Instrumentation and Methods for Astrophysics · Physics 2018-07-26 Jörg Herbel , Tomasz Kacprzak , Adam Amara , Alexandre Refregier , Aurelien Lucchi

We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…

Image and Video Processing · Electrical Eng. & Systems 2022-08-05 Bhargav Ghanekar , Vishwanath Saragadam , Dushyant Mehra , Anna-Karin Gustavsson , Aswin Sankaranarayanan , Ashok Veeraraghavan

We present a new algorithm for estimating the Point Spread Function (PSF) in wide-field astronomical images with extreme source crowding. Robust and accurate PSF estimation in crowded astronomical images dramatically improves the fidelity…

Image and Video Processing · Electrical Eng. & Systems 2021-02-09 Brendt Wohlberg , Przemek Wozniak

High quality, repeatable point-spread functions are important for science cases like direct exoplanet imaging, high-precision astrometry, and high-resolution spectroscopy of exoplanets. For such demanding applications, the initial on-sky…

Instrumentation and Methods for Astrophysics · Physics 2021-07-19 Steven P. Bos , Michael Bottom , Sam Ragland , Jacques-Robert Delorme , Sylvain Cetre , Laurent Pueyo

The past decade has brought many innovations in optical design for 3D super-resolution imaging of point-like emitters, but these methods often focus on single-emitter localization precision as a performance metric. Here, we propose a simple…

Optics · Physics 2022-09-28 James M. Jusuf , Matthew D. Lew

Among the approaches in three-dimensional (3D) single molecule localization microscopy, there are several point spread function (PSF) engineering approaches, in which depth information of molecules is encoded in 2D images. Usually,the…

Optics · Physics 2024-07-11 Hanzhe Wu , Danni Chen , YiHong Jiand Gan Xiang , Heng Li , Bin Yu , JunLe Qu

We present the implementation and use of algorithms for matching point-spread functions (PSFs) within the Pan-STARRS Image Processing Pipeline (IPP). PSF-matching is an essential part of the IPP for the detection of supernovae and…

Instrumentation and Methods for Astrophysics · Physics 2019-01-30 Paul A. Price , Eugene A. Magnier

A point spread function (PSF) describes the distribution of light for a pure point source in an astronomical image due to the optics of the instrument. An accurate PSF is key for deconvolution, point source photometry and source removal.…

Instrumentation and Methods for Astrophysics · Physics 2025-07-28 Ava Polzin

Point spread function (PSF) engineering of the emitter can code higher spatial frequency information of an image to break diffraction limit but suffer from the complexed optical systems. Here we present a robust strategy to simultaneously…

Optics · Physics 2021-02-23 Chaohao Chen , Baolei Liu , Yongtao Liu , Jiayan Liao , Xuchen Shan , Fan Wang , Dayong Jin

Instrumental aberrations strongly limit high-contrast imaging of exoplanets, especially when they produce quasistatic speckles in the science images. With the help of recent advances in deep learning, we have developed in previous works an…

Instrumentation and Methods for Astrophysics · Physics 2022-11-14 Maxime Quesnel , Gilles Orban de Xivry , Olivier Absil , Gilles Louppe

Accurate astrometry and photometry of saturated and coronagraphic point spread functions (PSFs) are fundamental to both ground- and space-based high contrast imaging projects. For ground-based adaptive optics imaging, differential…

Astrophysics · Physics 2008-11-26 C. Marois , D. Lafreniere , B. Macintosh , R. Doyon

Nanoparticles (NPs) are promising therapeutic delivery agents, yet it is increasingly apparent that the number and manner of presentation of cell binding ligands on the NP can affect the eventual fate of the therapeutic. Whenever NPs are…

Point spread function (PSF) reconstruction (PSF-R) is a well established technique to determine reliably and accurately the PSF from Adaptive Optics (AO) control loop data. We have successfully applied this technique to improve the…

Instrumentation and Methods for Astrophysics · Physics 2020-04-15 Olivier Beltramo-Martin , Antonino Marasco , Thierry Fusco , Davide Massari , Julien Milli , Giuliana Fiorentino , B. Neichel

Simulated images are essential in algorithm development and instrument testing for optical telescopes. During real observations, images obtained by optical telescopes are affected by spatially variable point spread functions (PSFs), a…

Instrumentation and Methods for Astrophysics · Physics 2025-02-17 Zeyu Bai , Peng Jia , Jiameng Lv , Xiang Zhang , Wennan Xiang , Lin Nie

Precise centroid estimation plays a critical role in accurate astrometry using telescope images. Conventional centroid estimation fits a template point spread function (PSF) to the image data. Because the PSF is typically not known to high…

Instrumentation and Methods for Astrophysics · Physics 2015-05-27 Chengxing Zhai , Mike Shao , Renaud Goullioud , Bijan Nemati

Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual…

Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…