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Histopathological imaging is vital for cancer research and clinical practice, with multiplexed Immunofluorescence (MxIF) and Hematoxylin and Eosin (H&E) providing complementary insights. However, aligning different stains at the cell level…

Image and Video Processing · Electrical Eng. & Systems 2024-10-02 Jun Jiang , Raymond Moore , Brenna Novotny , Leo Liu , Zachary Fogarty , Ray Guo , Markovic Svetomir , Chen Wang

In contemporary pathology, multiplexed immunofluorescence (mIF) and multiplex immunohistochemistry (mIHC) present both significant opportunities and challenges. These methodologies shed light on intricate tumor microenvironment…

Medical practitioners use a number of diagnostic tests to make a reliable diagnosis. Traditionally, Haematoxylin and Eosin (H&E) stained glass slides have been used for cancer diagnosis and tumor detection. However, recently a variety of…

Image and Video Processing · Electrical Eng. & Systems 2023-12-27 Abubakr Shafique , Morteza Babaie , Ricardo Gonzalez , H. R. Tizhoosh

Due to its superior efficiency in utilizing annotations and addressing gigapixel-sized images, multiple instance learning (MIL) has shown great promise as a framework for whole slide image (WSI) classification in digital pathology…

Quantitative Methods · Quantitative Biology 2023-07-14 Qiehe Sun , Jiawen Li , Jin Xu , Junru Cheng , Tian Guan , Yonghong He

Advancements in digital imaging technologies have sparked increased interest in using multiplexed immunofluorescence (mIF) images to visualise and identify the interactions between specific immunophenotypes with the tumour microenvironment…

Image and Video Processing · Electrical Eng. & Systems 2024-07-01 Piumi Sandarenu , Julia Chen , Iveta Slapetova , Lois Browne , Peter H. Graham , Alexander Swarbrick , Ewan K. A. Millar , Yang Song , Erik Meijering

While multiplex immunofluorescence (mIF) imaging provides deep, spatially-resolved molecular data, integrating this information with the morphological standard of Hematoxylin & Eosin (H&E) can be very important for obtaining complementary…

Quantitative Methods · Quantitative Biology 2025-08-08 Jillur Rahman Saurav , Mohammad Sadegh Nasr , Jacob M. Luber

Histopathological analysis is a cornerstone of cancer diagnosis, with Hematoxylin and Eosin (H&E) staining routinely acquired for every patient to visualize cell morphology and tissue architecture. On the other hand, multiplex…

Computer Vision and Pattern Recognition · Computer Science 2026-03-20 Guillaume Balezo , Roger Trullo , Albert Pla Planas , Etienne Decenciere , Thomas Walter

In the present study, we propose a novel case-based similar image retrieval (SIR) method for hematoxylin and eosin (H&E)-stained histopathological images of malignant lymphoma. When a whole slide image (WSI) is used as an input query, it is…

Multiplex immunofluorescence (mIF) imaging technology facilitates the study of the tumour microenvironment in cancer patients. Due to the capabilities of this emerging bioimaging technique, it is possible to statistically analyse, for…

Applications · Statistics 2023-07-07 Jonatan A. González , Julia Wrobel , Simon Vandekar , Paula Moraga

The rapid digitization of histopathology slides has opened up new possibilities for computational tools in clinical and research workflows. Among these, content-based slide retrieval stands out, enabling pathologists to identify…

Computer Vision and Pattern Recognition · Computer Science 2025-10-28 Hongyi Wang , Zhengjie Zhu , Jiabo Ma , Fang Wang , Yue Shi , Bo Luo , Jili Wang , Qiuyu Cai , Xiuming Zhang , Yen-Wei Chen , Lanfen Lin , Hao Chen

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

Immunohistochemistry (IHC) has transformed clinical pathology by enabling the visualization of specific proteins within tissue sections. However, traditional IHC requires one tissue section per stain, exhibits section-to-section…

Digital pathology (DP) is a new research area which falls under the broad umbrella of health informatics. Owing to its potential for major public health impact, in recent years DP has been attracting much research attention. Nevertheless, a…

Image and Video Processing · Electrical Eng. & Systems 2019-03-12 Xingzhi Yue , Neofytos Dimitriou , Ognjen Arandjelovic

Pathological features are the gold standard for tumor diagnosis, guiding treatment and prognosis. However, standard histopathological process is labor-intensive and time-consuming, while frozen sections have lower accuracy. Dynamic…

The popular use of histopathology images, such as hematoxylin and eosin (H&E), has proven to be useful in detecting tumors. However, moving such cancer cases forward for treatment requires accurate on the amount of the human epidermal…

Computer Vision and Pattern Recognition · Computer Science 2025-12-30 Olaide N. Oyelade , Oliver Hoxey , Yulia Humrye

Accurate diagnosis and treatment of complex diseases require integrating histological, molecular, and clinical data, yet in practice these modalities are often incomplete owing to tissue scarcity, assay cost, and workflow constraints.…

Computer Vision and Pattern Recognition · Computer Science 2026-04-07 Jinxi Xiang , Mingjie Li , Siyu Hou , Yijiang Chen , Xiangde Luo , Yuanfeng Ji , Xiang Zhou , Ehsan Adeli , Akshay Chaudhari , Curtis P. Langlotz , Kilian M. Pohl , Ruijiang Li

Multi-modal learning adeptly integrates visual and textual data, but its application to histopathology image and text analysis remains challenging, particularly with large, high-resolution images like gigapixel Whole Slide Images (WSIs).…

Computer Vision and Pattern Recognition · Computer Science 2024-05-29 Quan Liu , Ruining Deng , Can Cui , Tianyuan Yao , Vishwesh Nath , Yucheng Tang , Yuankai Huo

Digital pathology has fundamentally altered diagnostic workflows by enabling the computational analysis of gigapixel Whole Slide Images (WSIs), yet effectively deciphering their complex tumor microenvironments remains a formidable…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Xuemei Qiu , Dawei Fan , Yebin Huang , Yanping Chen , Lifang Wei

In this paper, we introduce a Variational Autoencoder (VAE) based training approach that can compress and decompress cancer pathology slides at a compression ratio of 1:512, which is better than the previously reported state of the art…

Conventional histopathology has long been essential for disease diagnosis, relying on visual inspection of tissue sections. Immunohistochemistry aids in detecting specific biomarkers but is limited by its single-marker approach, restricting…

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