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Precise segmentation and classification of cell instances are vital for analyzing the tissue microenvironment in histology images, supporting medical diagnosis, prognosis, treatment planning, and studies of brain cytoarchitecture. However,…

Computer Vision and Pattern Recognition · Computer Science 2025-02-14 Valentina Vadori , Jean-Marie Graïc , Antonella Peruffo , Livio Finos , Ujwala Kiran Chaudhari , Enrico Grisan

Quantitative phase imaging (QPI) is an emerging label-free technique that produces images containing morphological and dynamical information without contrast agents. Unfortunately, the phase is wrapped in most imaging system. Phase…

Image and Video Processing · Electrical Eng. & Systems 2021-09-01 Fangshu Yang , Thanh-an Pham , Nathalie Brandenberg , Matthias P. Lutolf , Jianwei Ma , Michael Unser

The integrity of the reconstructed human epidermis generated in vitro could be assessed using histological analyses combined with immunohistochemical staining of keratinocyte differentiation markers. Computer-based analysis of scanned…

Image and Video Processing · Electrical Eng. & Systems 2025-12-01 Dawid Zamojski , Agnieszka Gogler , Dorota Scieglinska , Michal Marczyk

Stains are essential in histopathology to visualize specific tissue characteristics, with Haematoxylin and Eosin (H&E) serving as the clinical standard. However, pathologists frequently utilize a variety of special stains for the diagnosis…

Computer Vision and Pattern Recognition · Computer Science 2026-02-11 Oskar Thaeter , Christian Grashei , Anette Haas , Elisa Schmoeckel , Han Li , Peter J. Schüffler

Hematoxylin and Eosin (H&E) stained Whole Slide Images (WSIs) are utilized for biopsy visualization-based diagnostic and prognostic assessment of diseases. Variation in the H&E staining process across different lab sites can lead to…

Image and Video Processing · Electrical Eng. & Systems 2020-11-24 Aman Shrivastava , Will Adorno , Yash Sharma , Lubaina Ehsan , S. Asad Ali , Sean R. Moore , Beatrice C. Amadi , Paul Kelly , Sana Syed , Donald E. Brown

For diagnosing melanoma, hematoxylin and eosin (H&E) stained tissue slides remains the gold standard. These images contain quantitative information in different magnifications. In the present study, we investigated whether deep…

Tissues and Organs · Quantitative Biology 2019-04-15 Peizhen Xie , Ke Zuo , Yu Zhang , Fangfang Li , Mingzhu Yin , Kai Lu

Histopathological image classification is an important task in medical image analysis. Recent approaches generally rely on weakly supervised learning due to the ease of acquiring case-level labels from pathology reports. However,…

Computer Vision and Pattern Recognition · Computer Science 2024-11-14 Bodong Zhang , Hamid Manoochehri , Man Minh Ho , Fahimeh Fooladgar , Yosep Chong , Beatrice S. Knudsen , Deepika Sirohi , Tolga Tasdizen

In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…

Optics · Physics 2020-12-17 Xi Chen , Mikhail E. Kandel , Gabriel Popescu

Histological visualizations are critical to clinical disease management and are fundamental to biological understanding. However, current approaches that rely on bright-field microscopy require extensive tissue preparation prior to imaging.…

Histopathological evaluation of tissue samples is a key practice in patient diagnosis and drug development, especially in oncology. Historically, Hematoxylin and Eosin (H&E) has been used by pathologists as a gold standard staining.…

Computer Vision and Pattern Recognition · Computer Science 2018-10-16 Amal Lahiani , Jacob Gildenblat , Irina Klaman , Shadi Albarqouni , Nassir Navab , Eldad Klaiman

Background and purpose: Colorectal cancer has become the third most common cancer worldwide, accounting for approximately 10% of cancer patients. Early detection of the disease is important for the treatment of colorectal cancer patients.…

Image and Video Processing · Electrical Eng. & Systems 2022-02-18 Weiming Hu , Chen Li , Xiaoyan Li , Md Mamunur Rahaman , Yong Zhang , Haoyuan Chen , Wanli Liu , Yudong Yao , Hongzan Sun , Ning Xu , Xinyu Huang , Marcin Grzegorze

Expression of human epidermal growth factor receptor 2 (HER2) is an important biomarker in breast cancer patients who can benefit from cost-effective automatic Hematoxylin and Eosin (H\&E) HER2 scoring. However, developing such scoring…

Computer Vision and Pattern Recognition · Computer Science 2024-11-11 Rawan S. Abdulsadig , Bryan M. Williams , Nikolay Burlutskiy

The human body is mysterious, consisting of innumerable fine and complex structures and a vast amount of information. The field of modern pathology has been systematized around the findings and observations obtained from light microscopy.…

Medical Physics · Physics 2021-11-29 Satoshi Kume

Optical absorption is a primary, label-defining contrast across biology, pathology, and materials science, yet three-dimensional quantitative absorption imaging has remained largely inaccessible to the brightfield microscopes used in…

Recovering both amplitude and phase information from a system is a fundamental goal of optical imaging. At the same time, it is crucial to operate at low photon doses to avoid altering the sample, particularly in biological applications.…

Quantum Physics · Physics 2026-01-15 Alberto Paniate , Giuseppe Ortolano , Sarika Soman , Marco Genovese , Ivano Ruo-Berchera

Large field-of-view (FOV) microscopic imaging with high lateral resolution (1-2 microns for high space-bandwidth product) plays a pivotal role in biomedicine and biophotonics, especially within the label-free regime, e.g., for whole slide…

Histopathology images; microscopy images of stained tissue biopsies contain fundamental prognostic information that forms the foundation of pathological analysis and diagnostic medicine. However, diagnostics from histopathology images…

Computer Vision and Pattern Recognition · Computer Science 2019-10-30 Aïcha BenTaieb , Ghassan Hamarneh

Variations in hematoxylin and eosin (H&E) stained images (due to clinical lab protocols, scanners, etc) directly impact the quality and accuracy of clinical diagnosis, and hence it is important to control for these variations for a reliable…

Image and Video Processing · Electrical Eng. & Systems 2020-06-26 Saad Nadeem , Travis Hollmann , Allen Tannenbaum

Standard of care diagnostic procedure for suspected skin cancer is microscopic examination of hematoxylin \& eosin stained tissue by a pathologist. Areas of high inter-pathologist discordance and rising biopsy rates necessitate higher…

The current standard for detecting human epidermal growth factor receptor 2 (HER2) status in breast cancer patients relies on HER2 amplification, identified through fluorescence in situ hybridization (FISH) or immunohistochemistry (IHC).…

Image and Video Processing · Electrical Eng. & Systems 2024-09-27 Ardhendu Sekhar , Vrinda Goel , Garima Jain , Abhijeet Patil , Ravi Kant Gupta , Tripti Bameta , Swapnil Rane , Amit Sethi