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In recent years, image-scanning microscopy (ISM, also termed pixel-reassignment microscopy) has emerged as a technique that improves the resolution and signal-to-noise compared to confocal and widefield microscopy by employing a detector…

Optics · Physics 2023-04-19 Tal I. Sommer , Gil Weinberg , Ori Katz

Endoscopy is the most widely used medical technique for cancer and polyp detection inside hollow organs. However, images acquired by an endoscope are frequently affected by illumination artefacts due to the enlightenment source orientation.…

Image and Video Processing · Electrical Eng. & Systems 2022-07-07 Axel Garcia-Vega , Ricardo Espinosa , Gilberto Ochoa-Ruiz , Thomas Bazin , Luis Eduardo Falcon-Morales , Dominique Lamarque , Christian Daul

The use of individual multimode optical fibers in endoscopy applications has the potential to provide highly miniaturized and noninvasive probes for microscopy and optical micromanipulation. A few different strategies have been proposed…

Confocal laser endomicroscopy (CLE) is a novel imaging modality that provides in vivo histological cross-sections of examined tissue. Recently, attempts have been made to develop miniaturized in vivo imaging devices, specifically confocal…

Computer Vision and Pattern Recognition · Computer Science 2018-06-22 Saeed Izadi , Kathleen P. Moriarty , Ghassan Hamarneh

We propose to use high numerical aperture single mode optical fibers like photonic crystal fiber for lensless in-line holographic microscopy. Highly divergent beam helps to overcome the spatial sampling limitation of the image sensor. In…

Optics · Physics 2020-07-01 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab K. Dutta

Lensless microscopy with coherent or partially coherent light sources is a well known imaging technique, commonly referred as digital in-line holographic microscopy. In the established methods, both the spatial and temporal coherence of…

Optics · Physics 2020-11-04 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

We report on a method to obtain confocal imaging through multimode fibers using optical correlation. First, we measure the fiber's transmission matrix in a calibration step. This allows us to create focused spots at one end of the fiber by…

Hyperspectral imaging is useful for applications ranging from medical diagnostics to agricultural crop monitoring; however, traditional scanning hyperspectral imagers are prohibitively slow and expensive for widespread adoption. Snapshot…

Image and Video Processing · Electrical Eng. & Systems 2020-09-30 Kristina Monakhova , Kyrollos Yanny , Neerja Aggarwal , Laura Waller

A lensless digital holography enables wide-field microscopic imaging without the limitations imposed by optical lens performance. However, conventional holographic imaging often relies on magnifying optical systems to compensate for the low…

Optics · Physics 2025-06-24 Byung Gyu Chae

Confocal imaging and phase imaging are powerful tools in life science research and industrial inspection. To coherently link the two techniques with different depth resolutions, we introduce an optical frequency comb (OFC) to microscopy.…

We introduce a conceptual framework for a lensless imaging system which employs an active metasurface as a high-frequency, continuously tunable amplitude and phase modulation aperture, coupled to a discrete single-pixel detector. Using an…

Optics · Physics 2024-11-14 Julie Belleville , Prachi Thureja , Harry A. Atwater

Imaging through a single optical fiber offers attractive possibilities in many applications such as microendoscopy or remote sensing. However, the direct transmission of an image through an optical fiber is difficult because spatial…

Optics · Physics 2015-05-29 Roman Barankov , Jerome Mertz

Mask-based lensless imaging uses an optical encoder (e.g. a phase or amplitude mask) to capture measurements, then a computational decoding algorithm to reconstruct images. In this work, we evaluate and design lensless encoders based on the…

Lensless imaging seeks to replace/remove the lens in a conventional imaging system. The earliest cameras were in fact lensless, relying on long exposure times to form images on the other end of a small aperture in a darkened room/container…

Image and Video Processing · Electrical Eng. & Systems 2022-06-06 Eric Bezzam , Sepand Kashani , Martin Vetterli , Matthieu Simeoni

Wide-field fluorescence microscopy, while much faster than confocal microscopy, suffers from a lack of optical sectioning and poor axial resolution. 3D structured illumination microscopy (SIM) has been demonstrated to provide optical…

Fast, volumetric imaging over large scales has been a long-standing goal in biological microscopy. Scanning techniques such as fluorescence confocal microscopy can acquire 2D images at high resolution and high speed, but extending the…

Tissue stiffness is related to soft tissue pathologies and can be assessed through palpation or via clinical imaging systems, e.g., ultrasound or magnetic resonance imaging. Typically, the image based approaches are not suitable during…

Signal Processing · Electrical Eng. & Systems 2025-09-04 Maximilian Neidhardt , Sarah Latus , Tim Eixmann , Gereon Hüttmann , Alexander Schlaefer

X-ray imaging is a fundamental tool of routine clinical diagnosis. Fluoroscopic imaging can further acquire X-ray images at video frame rates, thus enabling non-invasive in-vivo motion studies of joints, gastrointestinal tract, etc. For…

Image and Video Processing · Electrical Eng. & Systems 2018-10-22 Jacky C. K. Chow , Derek Lichti , Kathleen Ang , Gregor Kuntze , Gulshan Sharma , Janet Ronsky

We present a novel fiber-optic component, a "tapered multi-core fiber (MCF)", designed for integration into ultra-miniaturized endoscopes for minimally invasive two-photon point-scanning imaging and to address the power delivery issue that…

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin
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