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In the analysis of spatially resolved transcriptomics data, detecting spatially variable genes (SVGs) is crucial. Numerous computational methods exist, but varying SVG definitions and methodologies lead to incomparable results. We review 33…

Quantitative Methods · Quantitative Biology 2024-10-04 Guanao Yan , Shuo Harper Hua , Jingyi Jessica Li

Spatial variable genes (SVGs) reveal critical information about tissue architecture, cellular interactions, and disease microenvironments. As spatial transcriptomics (ST) technologies proliferate, accurately identifying SVGs across diverse…

Applications · Statistics 2025-10-21 Jiawen Chen , Jinwei Zhang , Dongshen Peng , Yutong Song , Aitong Ruan , Yun Li , Didong Li

Gene expression profiling technologies have been used in various applications such as cancer biology. The development of gene expression profiling has expanded the scope of target discovery in transcriptomic studies, and each technology…

Genomics · Quantitative Biology 2023-01-10 Hyeongseon Jeon , Juan Xie , Yeseul Jeon , Kyeong Joo Jung , Arkobrato Gupta , Won Chang , Dongjun Chung

Network models provide a powerful framework for analysing single-cell count data, facilitating the characterisation of cellular identities, disease mechanisms, and developmental trajectories. However, uncertainty modeling in unsupervised…

Genomics · Quantitative Biology 2026-04-27 Shanshan Ren , Thomas E. Bartlett , Lina Gerontogianni , Swati Chandna

Understanding how cellular morphology, gene expression, and spatial context jointly shape tissue function is a central challenge in biology. Image-based spatial transcriptomics technologies now provide high-resolution measurements of cell…

Quantitative Methods · Quantitative Biology 2026-02-17 Zhenglun Kong , Mufan Qiu , John Boesen , Xiang Lin , Sukwon Yun , Tianlong Chen , Manolis Kellis , Marinka Zitnik

Understanding the dynamic nature of biological systems is fundamental to deciphering cellular behavior, developmental processes, and disease progression. Single-cell RNA sequencing (scRNA-seq) has provided static snapshots of gene…

Quantitative Methods · Quantitative Biology 2025-05-02 Zhenyi Zhang , Yuhao Sun , Qiangwei Peng , Tiejun Li , Peijie Zhou

Biological systems commonly exhibit complex spatiotemporal patterns whose underlying generative mechanisms pose a significant analytical challenge. Traditional approaches to spatiodynamic inference rely on dimensionality reduction through…

Quantitative Methods · Quantitative Biology 2025-08-01 Jun Won Park , Kangyu Zhao , Sanket Rane

In this paper, we propose a Bayesian matrix-variate spatiotemporal modeling framework for jointly analyzing multiple response variables observed at spatial locations over time. The approach relaxes the standard assumption of spatial…

Methodology · Statistics 2026-04-23 Rodrigo de Souza Bulhões , Marina Silva Paez , Dani Gamerman

Spatial studies of transcriptome provide biologists with gene expression maps of heterogeneous and complex tissues. However, most experimental protocols for spatial transcriptomics suffer from the need to select beforehand a small fraction…

Machine Learning · Computer Science 2019-05-08 Romain Lopez , Achille Nazaret , Maxime Langevin , Jules Samaran , Jeffrey Regier , Michael I. Jordan , Nir Yosef

Advances in spatial transcriptomics (ST) technologies enable systematic molecular characterization of tumor microenvironment, tumor gradients and gene regulatory networks. Cancer progression is known to vary along pathological gradients,…

Single-cell-resolution spatial transcriptomics profiles gene expression at cellular locations in native tissues, yet accurate cell-type annotation remains challenging: imaging-based platforms are constrained by targeted gene panels, whereas…

Cell Behavior · Quantitative Biology 2026-05-27 Yiyang Zhang , Bokai Zhao , Xiaoru Zhang , Zongchang Du , Xiaojuan Sun , Tianzi Jiang

The advent of next-generation sequencing-based spatially resolved transcriptomics (SRT) techniques has reshaped genomic studies by enabling high-throughput gene expression profiling while preserving spatial and morphological context.…

Applications · Statistics 2023-12-14 Bencong Zhu , Guanyu Hu , Yang Xie , Lin Xu , Xiaodan Fan , Qiwei Li

Spatial Transcriptomics is a novel technology that aligns histology images with spatially resolved gene expression profiles. Although groundbreaking, it struggles with gene capture yielding high corruption in acquired data. Given potential…

Computer Vision and Pattern Recognition · Computer Science 2024-09-30 Gabriel Mejia , Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Pablo Arbeláez

Complex spatial and temporal patterns of gene expression underlie embryo differentiation, yet methods do not yet exist for the efficient genome-wide determination of spatial expression patterns during development. In situ imaging of…

Genomics · Quantitative Biology 2013-08-15 Peter A. Combs , Michael B. Eisen

Spatial transcriptomics methods capture cellular measurements such as gene expression and cell types at specific locations in a cell, helping provide a localized picture of tissue health. Traditional visualization techniques superimpose the…

Quantitative Methods · Quantitative Biology 2024-10-16 Siyuan Zhao , G. Elisabeta Marai

Spatiotemporal gene expression data of the human brain offer insights on the spa- tial and temporal patterns of gene regulation during brain development. Most existing methods for analyzing these data consider spatial and temporal profiles…

Methodology · Statistics 2017-02-27 Tianqi Liu , Ming Yuan , Hongyu Zhao

Many cellular responses to surrounding cues require temporally concerted transcriptional regulation of multiple genes. In prokaryotic cells, a single-input-module motif with one transcription factor regulating multiple target genes can…

Subcellular Processes · Quantitative Biology 2019-06-19 Jingyu Zhang , Hengyu Chen , Ruoyan Li , David A. Taft , Guang Yao , Fan Bai , Jianhua Xing

The technology to generate Spatially Resolved Transcriptomics (SRT) data is rapidly being improved and applied to investigate a variety of biological tissues. The ability to interrogate how spatially localised gene expression can lend new…

Quantitative Methods · Quantitative Biology 2021-08-04 Natalie Charitakis , Mirana Ramialison , Hieu T. Nim

Single-cell gene expression measurements encode variability spanning molecular noise, cell-to-cell heterogeneity, and technical artifacts. Mechanistic stochastic models provide powerful approaches to disentangle these sources, yet inferring…

Quantitative Methods · Quantitative Biology 2025-09-19 Christopher E. Miles

The tumor microenvironment (TME) is a spatially heterogeneous ecosystem where cellular interactions shape tumor progression and response to therapy. Multiplexed imaging technologies enable high-resolution spatial characterization of the…

Applications · Statistics 2025-04-04 Joel Eliason , Arvind Rao , Timothy L Frankel , Michele Peruzzi