Related papers: Optimal calibration of optical tweezers with arbit…
Optical tweezers are highly versatile laser traps for neutral microparticles, with fundamental applications in physics and in single molecule cell biology. Force measurements are performed by converting the stiffness response to…
Optical tweezers find applications in various fields, ranging from biology to physics. One of the fundamental steps necessary to perform quantitative measurements using trapped particles is the calibration of the tweezer's spring constant.…
Emulsions are ubiquitous in everyday life and find applications in various industries. Optical tweezers (OTs) have emerged as the preferred method for studying emulsion dynamics. In this review, we first introduce the theory of optical…
We explain and demonstrate a new method of force- and position-calibration for optical tweezers with back-focal-plane photo detection. The method combines power spectral measurements of thermal motion and the response to a sinusoidal motion…
Recently, acoustic tweezers based on an array of ultrasonic transducers have been reported taking inspiration from holographic optical tweezers. In the latter technique, the calibration of the optical trap is an essential procedure to…
Dual trap optical tweezers are often used in high-resolution measurements in single-molecule biophysics. Such measurements can be hindered by the presence of extraneous noise sources, the most prominent of which is the coupling of…
Optically levitated dielectric nanoparticles have become valuable tools for precision sensing and quantum optomechanical experiments. To predict the dynamic properties of a particle trapped in an optical tweezer with high fidelity, a tool…
In recent years, levitated particles of optical traps in vacuum have shown enormous potential in precision sensor development and searching for new physics. The accuracy of the calibration relating the detected signal to absolute…
Fast and accurate 3D position detection in optical tweezers (OT) is essential for quantitatively monitoring subtle variations in the mechanical properties of microscopic systems ranging from biomolecules to cells and colloids. Because…
Since their invention in the 1980s [1], optical tweezers have found a wide range of applications, from biophotonics and mechanobiology to microscopy and optomechanics [2, 3, 4, 5]. Simulations of the motion of microscopic particles held by…
Optical tweezers equipped with position detection allow for application of piconewton-scale forces and high-temporal-resolution measurements of nanometer-scale motion. While typically used for trapping microscopic objects, the optical…
Optical tweezers enable non-contact trapping of micro-scale objects using light. Despite their widespread use, it is currently not known how tightly it is possible to three-dimensionally trap micro-particles with a given photon budget.…
Optical tweezers exploit light--matter interactions to trap particles ranging from single atoms to micrometer-sized eukaryotic cells. For this reason, optical tweezers are a ubiquitous tool in physics, biology, and nanotechnology. Recently,…
Since their invention in 1986 by Arthur Ashkin and colleagues, optical tweezers have become an essential tool in several fields of physics, spectroscopy, biology, nanotechnology, and thermodynamics. In this Tutorial, we provide a primer on…
Calibration of optically trapped particles in-vivo has been complicated given the frequency dependence and spatial inhomogeneity of the cytoplasmic viscosity, and the requirement of accurate knowledge of the medium refractive index.…
Normal thermal fluctuations of the cell membrane have been studied extensively using high resolution microscopy and focused light, particularly at the peripheral regions of a cell. We use a single probe particle attached non-specifically to…
Optical tweezers have found widespread application in many fields, from physics to biology. Here, we explain in detail how optical forces and torques can be described within the geometrical optics approximation and we show that this…
In spite of the widespread use of optical tweezers as a quantitative tool to measure small forces, there exists no unambiguous and simple experimental method for either validating its theoretically predicted form or empirically…
We present a novel approach to determine the optical properties of materials in the nanoscale range using optical tweezers (OT). Fluorescent polymer-based nanostructures (pdots) are optically trapped in a Gaussian beam OT and the trap…
We present a dual-trap optical tweezers setup which directly measures forces using linear momentum conservation. The setup uses a counter-propagating geometry, which allows momentum measurement on each beam separately. The experimental…