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Fluorescence Molecular Tomography (FMT) is a promising technique for non-invasive 3D visualization of fluorescent probes, but its reconstruction remains challenging due to the inherent ill-posedness and reliance on inaccurate or…

Image and Video Processing · Electrical Eng. & Systems 2025-11-07 Shihan Zhao , Jianru Zhang , Yanan Wu , Linlin Li , Siyuan Shen , Xingjun Zhu , Guoyan Zheng , Jiahua Jiang , Wuwei Ren

Fluorescence molecular tomography (FMT) is a sensitive optical imaging technology widely used in biomedical research. However, the ill-posedness of the inverse problem poses a huge challenge to FMT reconstruction. Although end-to-end deep…

Image and Video Processing · Electrical Eng. & Systems 2025-03-18 Peng Zhang , Qianqian Xue , Xingyu Liu , Guanglei Zhang , Wenjian Wang , Jiye Liang

Fluorescence molecular tomography (FMT) is an emerging powerful tool for biomedical research. There are two factors that influence FMT reconstruction most effectively. The first one is the regularization techniques. Traditional methods such…

Image and Video Processing · Electrical Eng. & Systems 2021-09-09 Yan Liu , Wuwei Ren , Habib Ammari

This paper concerns an inverse problem for fluorescence diffuse optical tomography (FDOT) reconstructing locations of multiple point targets from the measured temporal response functions. The targets are multiple fluorescent point objects…

Numerical Analysis · Mathematics 2024-11-26 Shuli Chen , Junyong Eom , Gen Nakamura , Goro Nishimura

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique in biomedical research that uses the fluorophore decay rate to provide additional contrast in fluorescence microscopy. However, at present, the calculation, analysis,…

Image and Video Processing · Electrical Eng. & Systems 2021-06-09 Varun Mannam , Yide Zhang , Xiaotong Yuan , Cara Ravasio , Scott S. Howard

Fluorescence molecular tomography (FMT) has potential of providing high contrast images for breast tumor detection. Computational phantom provides a convenient way to a wide variety of fluorophore distribution configurations in patients and…

Medical Physics · Physics 2018-04-02 Yansong Zhu , Abhinav K. Jha , Arman Rahmim

We present a reconstruction method involving maximum-likelihood expectation maximization (MLEM) to model Poisson noise as applied to fluorescence molecular tomography (FMT). MLEM is initialized with the output from a sparse…

Medical Physics · Physics 2018-04-03 Yansong Zhu , Abhinav K. Jha , Dean F. Wong , Arman Rahmim

Compressive Macroscopic Fluorescence Lifetime Imaging (MFLI) is a novel technical implementation that enables monitoring multiple molecular interactions in macroscopic scale. Especially, we reported recently on the development of a…

Medical Physics · Physics 2018-02-23 Ruoyang Yao , Marien Ochoa , Xavier Intes , Pingkun Yan

We present a fast and accurate analytical method for fluorescence lifetime imaging microscopy (FLIM) using the extreme learning machine (ELM). We used extensive metrics to evaluate ELM and existing algorithms. First, we compared these…

Biological Physics · Physics 2022-03-28 Zhenya Zang , Dong Xiao , Quan Wang , Zinuo Li , Wujun Xie , Yu Chen , David Day Uei Li

Fluorescence photoacoustic tomography (fPAT) is a molecular imaging modality that combines photoacoustic tomography (PAT) with fluorescence imaging to obtain high-resolution imaging of fluorescence distributions inside heterogeneous media.…

Analysis of PDEs · Mathematics 2019-09-27 Kui Ren , Rongting Zhang , Yimin Zhong

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Fluorescence Molecular Tomography (FMT) is a widely used non-invasive optical imaging technology in biomedical research. It usually faces significant accuracy challenges in depth reconstruction, and conventional iterative methods struggle…

Numerical Analysis · Mathematics 2025-10-08 Ruchi Guo , Jiahua Jiang , Bangti Jin , Wuwei Ren , Jianru Zhang

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

FRET measurements can provide dynamic spatial information on length scales smaller than the diffraction limit of light. Several methods exist to measure FRET between fluorophores, including Fluorescence Lifetime Imaging Microscopy (FLIM),…

Quantitative Methods · Quantitative Biology 2017-01-09 Bryan Kaye , Peter J. Foster , Tae Yeon Yoo , Daniel J. Needleman

We investigate a hybrid inverse problem in fluorescence ultrasound modulated optical tomography (fUMOT) in the diffusive regime. We prove that the absorption coefficient of the fluorophores at the excitation frequency and the quantum…

Analysis of PDEs · Mathematics 2018-04-05 Wei Li , Yang Yang , Yimin Zhong

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

Recent advances in cancer research largely rely on new developments in microscopic or molecular profiling techniques offering high level of detail with respect to either spatial or molecular features, but usually not both. Here, we present…

Fluorescence spectroscopy and modeling provide powerful means to characterize biomacromolecular structures, dynamics, and interactions. F\"orster resonance energy transfer serves as a key technique for this due to its nanometer-scale…

Biological Physics · Physics 2026-02-26 Thomas-Otavio Peulen , Daria Maksutova , Thorben Cordes
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