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The point spread function (PSF) is fundamental to any type of microscopy, most importantly so for single-molecule localization techniques, where the exact PSF shape is crucial for precise molecule localization at the nanoscale. However,…
In single molecule orientation localization microscopy, valuable information about the orientation and longitudinal position of each molecule is often encoded in the shape of the point spread function (PSF). This shape, though, can be…
We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…
In Real-Time, Feedback-Driven Single Particle Tracking methods, measurements of the emission intensity from a labeled, nanometer-scale particle are used in a feedback loop to track the motion of the particle as it moves inside its native…
Point Spread Function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline…
Surface plasmon resonances (SPRs) are crucial for confining light beyond the diffraction limit, yet heavy metal losses often limit their spectral localization. Here, we propose a practical strategy for enabling the spectral localization of…
Nanoscale localization of point emitters is critical to several methods in optical fluorescence microscopy, including single-molecule super-resolution imaging and tracking. While the precision of the localization procedure has been the…
Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…
The single sideband (SSB) framework of analytical electron ptychography can account for the presence of residual geometrical aberrations induced by the probe-forming lens. However, the accuracy of this aberration correction method is highly…
Single Particle Tracking (SPT) is a powerful class of tools for analyzing the dynamics of individual biological macromolecules moving inside living cells. The acquired data is typically in the form of a sequence of camera images that are…
Point-spread-function (PSF) engineering is a well-established computational imaging technique that uses phase masks and other optical elements to embed extra information (e.g., depth) into the images captured by conventional CMOS image…
Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the point spread function in iSCAT by a Gaussian intensity…
Super-resolutive 3D tracking, such as PSF engineering or evanescent field imaging has long been used to track microparticles and to enhance the throughput of single molecules force spectroscopic measurements. However, current methods…
Point-spread-function (PSF) engineering is a powerful computational imaging techniques wherein a custom phase mask is integrated into an optical system to encode additional information into captured images. Used in combination with deep…
Scattering-based single-particle tracking (S-SPT) has opened new avenues for highly sensitive label-free detection and characterization of nanoscopic objects, making it particularly attractive for various analytical applications. However, a…
Particle tracking, which is an essential tool in many fields of scientific research, uses algorithms that retrieve the centroid of tracked particles with sub-pixel accuracy. However, images in which the particles occupy a small number of…
A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…
Interferometric scattering (iSCAT) microscopy is currently among the most powerful techniques available for achieving high-sensitivity single-particle localization. This capability is realized through homodyne detection, where interference…
In astronomy or biological imaging, refractive index inhomogeneities of e.g. atmosphere or tissues induce optical aberrations which degrade the desired information hidden behind the medium. A standard approach consists in measuring these…
The Point Spread Function (PSF) is a key figure of merit for specifying the angular resolution of optical systems and, as the demand for higher and higher angular resolution increases, the problem of surface finishing must be taken…