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Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a wide range of cell types. Fluorescence polarization spectroscopy tools have also enabled…

Biological Physics · Physics 2023-01-25 Jack W Shepherd , Alex L Payne-Dwyer , Ji-Eun Lee , Aisha Syeda , Mark C Leake

Proteins are commonly used by biochemical industry for numerous processes. Refining these proteins' properties via mutations causes stability effects as well. Accurate computational method to predict how mutations affect protein stability…

Machine Learning · Statistics 2018-03-26 Emmi Jokinen , Markus Heinonen , Harri Lähdesmäki

High-precision measurements require optimal setups and analysis tools to achieve continuous improvements. Systematic corrections need to be modeled with high accuracy and known uncertainty to reconstruct underlying physical phenomena. To…

Data Analysis, Statistics and Probability · Physics 2022-05-31 Max Lamparth , Mattis Bestehorn , Bastian Märkisch

Modern microscopy techniques are developing towards high-resolution imaging, and tremendous progress has been made in past decades; however, the imaging of individual biological macromolecules at atomic resolution using short-wavelength…

Optics · Physics 2022-01-12 Tatiana Latychevskaia

A crucial step in single particle analysis (SPA) of cryogenic electron microscopy (Cryo-EM), 2D classification and alignment takes a collection of noisy particle images to infer orientations and group similar images together. Averaging…

Computer Vision and Pattern Recognition · Computer Science 2022-06-28 Supawit Chockchowwat , Chandrajit L. Bajaj

A main task in cryo-electron microscopy single particle reconstruction is to find a three-dimensional model of a molecule given a set of its randomly oriented and positioned noisy projection-images. In this work, we propose an algorithm for…

Computational Physics · Physics 2022-11-11 Adi Shasha Geva , Yoel Shkolnisky

In this paper we address the problem of building a class of robust factorization algorithms that solve for the shape and motion parameters with both affine (weak perspective) and perspective camera models. We introduce a Gaussian/uniform…

Computer Vision and Pattern Recognition · Computer Science 2020-12-16 Andrei Zaharescu , Radu Horaud

Single-particle cryo-electron microscopy (cryo-EM) has become a cornerstone of structural biology, enabling near-atomic resolution analysis of macromolecules through advanced computational methods. However, the development of cryo-EM…

Computer Vision and Pattern Recognition · Computer Science 2025-10-07 Runmin Jiang , Genpei Zhang , Yuntian Yang , Siqi Wu , Minhao Wu , Wanyue Feng , Yizhou Zhao , Xi Xiao , Xiao Wang , Tianyang Wang , Xingjian Li , Muyuan Chen , Min Xu

A theoretical scheme for the treatment of an open molecular system with electrons and nuclei is proposed. The idea is based on the Grand Canonical description of a quantum region embedded in a classical reservoir of molecules. Electronic…

Statistical Mechanics · Physics 2018-01-17 Luigi Delle Site

Bayesian model updating based on Gaussian Process (GP) models has received attention in recent years, which incorporates kernel-based GPs to provide enhanced fidelity response predictions. Although most kernel functions provide high fitting…

Recent breakthroughs in high-resolution imaging of biomolecules in solution with cryo-electron microscopy (cryo-EM) have unlocked new doors for the reconstruction of molecular volumes, thereby promising further advances in biology,…

Image and Video Processing · Electrical Eng. & Systems 2022-05-27 Claire Donnat , Axel Levy , Frederic Poitevin , Ellen Zhong , Nina Miolane

Gaussian mixtures are a common density representation in nonlinear, non-Gaussian Bayesian state estimation. Selecting an appropriate number of Gaussian components, however, is difficult as one has to trade of computational complexity…

Systems and Control · Computer Science 2012-04-02 Marco F. Huber

We report applications of analytical formalisms and Molecular Dynamics (MD) simulations to the calculation of redox entropy of plastocyanin metalloprotein in aqueous solution. The goal of our analysis is to establish critical components of…

Biological Physics · Physics 2009-11-13 David N. LeBard , Dmitry V. Matyushov

We propose a method to reconstruct the 3-D molecular structure from micrographs collected at just one sample tilt angle in the random conical tilt scheme in cryo-electron microscopy. Our method uses autocorrelation analysis on the…

Data Analysis, Statistics and Probability · Physics 2021-01-12 Ti-Yen Lan , Nicolas Boumal , Amit Singer

The understanding of protein structure, folding, and interaction with other proteins remains one of the grand challenges of modern biology. Tremendous progress has been made thanks to X-ray- or electron-based techniques that have provided…

Particle identification in large high-energy physics experiments typically relies on classifiers obtained by combining many experimental observables. Predicting the probability density function (pdf) of such classifiers in the multivariate…

High Energy Physics - Experiment · Physics 2022-02-11 Giacomo Graziani , Lucio Anderlini , Saverio Mariani , Edoardo Franzoso , Luciano Libero Pappalardo , Pasquale di Nezza

Genomic signal processing has been used successfully in bioinformatics to analyze biomolecular sequences and gain varied insights into DNA structure, gene organization, protein binding, sequence evolution, etc. But challenges remain in…

Genomics · Quantitative Biology 2022-11-04 Saish Jaiswal , Shreya Nema , Hema A Murthy , Manikandan Narayanan

Modern electron tomography has progressed to higher resolution at lower doses by leveraging compressed sensing methods that minimize total variation (TV). However, these sparsity-emphasized reconstruction algorithms introduce tunable…

Medical Physics · Physics 2023-09-12 William Millsaps , Jonathan Schwartz , Zichao Wendy Di , Yi Jiang , Robert Hovden

This paper presents a new Bayesian model and algorithm for nonlinear unmixing of hyperspectral images. The model proposed represents the pixel reflectances as linear combinations of the endmembers, corrupted by nonlinear (with respect to…

Methodology · Statistics 2015-10-06 Yoann Altmann , Marcelo Pereyra , Stephen McLaughlin

The standard technique for sub-pixel estimation of atom positions from atomic resolution scanning transmission electron microscopy images relies on fitting intensity maxima or minima with a two-dimensional Gaussian function. While this is a…

Materials Science · Physics 2020-01-28 Debangshu Mukherjee , Leixin Miao , Greg Stone , Nasim Alem