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Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

In Super-resolution, a varying-illumination image stack is required. This enriched the dataset typically necessitates precise mechanical control and micron scale optical alignment and repeatability. Here, we introduce a novel methodology…

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of…

Optics · Physics 2022-10-26 Geng Wang , Stephen A. Boppart , Haohua Tu

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

Microscopy images are powerful tools and widely used in the majority of research areas, such as biology, chemistry, physics and materials fields by various microscopies (scanning electron microscope (SEM), atomic force microscope (AFM) and…

Computer Vision and Pattern Recognition · Computer Science 2020-02-25 Israel Goytom , Qin Wang , Tianxiang Yu , Kunjie Dai , Kris Sankaran , Xinfei Zhou , Dongdong Lin

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast images of 2-D sections within bulk tissue. However, scattering limits their application to depths only up to ~1 millimeter. Multimode fibers make excellent…

Optics · Physics 2022-02-23 Sakshi Singh , Simon Labouesse , Rafael Piestun

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…

Fluorescence microscopy is essential to study biological structures and dynamics. However, existing systems suffer from a tradeoff between field-of-view (FOV), resolution, and complexity, and thus cannot fulfill the emerging need of…

Optics · Physics 2022-09-09 Yujia Xue , Qianwan Yang , Guorong Hu , Kehan Guo , Lei Tian

Imaging beyond the diffraction limit barrier has attracted wide attention due to the ability to resolve image features that were previously hidden. Of the various super-resolution microscopy techniques available, a particularly simple…

Scanning transmission X-ray microscopy (STXM) is a nanoscale imaging technique that can utilize several powerful contrast mechanisms for the quantitative mapping of chemical and physical materials properties. Spatial resolutions down to…

Instrumentation and Detectors · Physics 2026-05-27 Simone Finizio , Benjamin Watts , Benedikt Rösner , Tim A. Butcher , Sebastian Wintz , Markus Weigand , Jörg Raabe

Fourier light-field microscopy (FLFM) uses a micro-lens array (MLA) to segment the Fourier Plane of the microscopic objective lens to generate multiple two-dimensional perspective views, thereby reconstructing the three-dimensional(3D)…

Optics · Physics 2022-08-29 Haixin Huang , Haoyuan Qiu , Hanzhe Wu , Yihong Ji , Heng Li , Bin Yu , Danni Chen , Junle Qu

Wide-field high-resolution microscopy requires fast scanning and accurate image mosaicking to cover large fields of view without compromising image quality. However, conventional galvanometric scanning, particularly under sinusoidal…

Image and Video Processing · Electrical Eng. & Systems 2026-02-04 Ziyi Huang , Rosalinda Xiong , Yaning Wang , Jinglun Yu , Jin U. Kang

Visual inspection of solar modules is an important monitoring facility in photovoltaic power plants. Since a single measurement of fast CMOS sensors is limited in spatial resolution and often not sufficient to reliably detect small defects,…

Computer Vision and Pattern Recognition · Computer Science 2021-05-06 Mathis Hoffmann , Thomas Köhler , Bernd Doll , Frank Schebesch , Florian Talkenberg , Ian Marius Peters , Christoph J. Brabec , Andreas Maier , Vincent Christlein

Super-resolved far-field microscopy has emerged as a powerful tool for investigating the structure of objects with resolution well below the diffraction limit of light. Nearly all super-resolution imaging techniques reported to date rely on…

Spatial light modulators (SLMs) are the most relevant technology for dynamic wavefront manipulation. They find diverse applications ranging from novel displays to optical and quantum communications. Among commercial SLMs for phase…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

Optical manipulation techniques offer exceptional contactless control but are fundamentally limited in their ability to perform parallel multitasking. To achieve high-density, versatile manipulation with subwavelength photonic devices, it…

This article experimentally examines different configurations of a novel multi-camera array microscope (MCAM) imaging technology. The MCAM is based upon a densely packed array of "micro-cameras" to jointly image across a large field-of-view…