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Adenosine triphosphate (ATP) is a highly energetic molecule used as a coenzyme in the transfer of chemical energy required for the continuation of cellular metabolism. In addition, extracellular ATP has an important role in cell-to-cell…

Medical Physics · Physics 2018-10-17 Mustafa Sen

Optogenetic modulation of adenosine triphosphatase (ATPase) expression represents a novel approach to maximize bioprocess efficiency by leveraging enforced adenosine triphosphate (ATP) turnover. In this study, we experimentally implement a…

Over the last ten years, Patient-Derived Organoids (PDOs) emerged as the most reliable technology to generate ex-vivo tumor avatars. PDOs retain the main characteristics of their original tumor, making them a system of choice for…

Adenosine 5'-triphosphate (ATP) is the nearly ubiquitous "energy currency" of living organisms, and thus is a crucial participant in the majority of enzymatic reactions. The standard models in enzyme kinetics generally ignore the temporal…

Molecular Networks · Quantitative Biology 2014-06-10 Jasmine Nirody , Padmini Rangamani

Site-specific information on how adenosine triphosphate in the aqueous phase (ATP$_{(aq)}$) interacts with magnesium (Mg$^{2+}_{(aq)}$) is a prerequisite to understanding its complex biochemistry. To gather such information, we apply…

Recent studies reported that adenosine triphosphate (ATP) could inhibit as well as enhance the phase separation in prion-like proteins. The molecular mechanism underlying such a puzzling phenomenon remains elusive. Here, taking the fused in…

Soft Condensed Matter · Physics 2022-09-22 Chun-Lai Ren , Yue Shan , Pengfei Zhang , Hong-Ming Ding , Yu-qiang Ma

Transient absorption (TA) spectroscopy has proved fundamental to our understanding of energy and charge transfer in biological systems, allowing measurements of photoactive proteins on sub-picosecond timescales. Recently, ultrafast TA…

In this paper, we demonstrate that atom probe tomography (APT) can be applied to small-molecule organic materials. We show that APT can provide an unprecedented combination of mass resolution of $\lt 1~\mathrm{Da}$, spatial resolution of…

Bone structure is generally hierarchically organized into organic (collagen, proteins,...), inorganic (hydroxyapatite (HAP)) components. However, many fundamental mechanisms of the biomineralization processes such as HAP formation, the…

Structure and function in nanoscale atomistic assemblies are tightly coupled, and every atom with its specific position and even every electron will have a decisive effect on the electronic structure, and hence, on the molecular properties.…

Chemical Physics · Physics 2024-02-21 Katja-Sophia Csizi , Markus Reiher

Mismatch repair is a critical step in DNA replication that occurs after base selection and proofreading, significantly increasing fidelity. However, the mechanism of mismatch recognition has not been established for any repair enzyme.…

Biological Physics · Physics 2024-04-09 Nianqin Zhang , Yongjun Zhang

Atom probe tomography (APT) is a 3D analysis technique that offers unique chemical accuracy and sensitivity with sub-nanometer spatial resolution. Recently, there is an increasing interest in the application of APT to complex oxides…

Endoscopy is the most widely used medical technique for cancer and polyp detection inside hollow organs. However, images acquired by an endoscope are frequently affected by illumination artefacts due to the enlightenment source orientation.…

Image and Video Processing · Electrical Eng. & Systems 2022-07-07 Axel Garcia-Vega , Ricardo Espinosa , Gilberto Ochoa-Ruiz , Thomas Bazin , Luis Eduardo Falcon-Morales , Dominique Lamarque , Christian Daul

This article presents an algorithm for the evaluation of organelles' movements inside of an unmodified live cell. We used a time-lapse image series obtained using wide-field bright-field photon transmission microscopy as an algorithm input.…

Quantitative Methods · Quantitative Biology 2016-12-14 Renata Rychtarikova , Dalibor Stys

The anisotropic effective mass of electrons is directly measured using time-resolved THz- pump/THz-probe techniques in a n-doped InGaAs semiconductor thin film. A microscopic theory is used to attribute this anisotropy in the THz probe…

Materials Science · Physics 2010-11-16 F. Blanchard , D. Golde , F. H. Su , L. Razzari , G. Sharma , R. Morandotti , T. Ozaki , M. Reid , M. Kira , S. W. Koch , F. A. Hegmann

Expressing the energy content of food as the heat energy released by its combustion is potentially misleading. Food is used to produce adenosine triphosphate (ATP). The free energy of conversion of ATP into adenosine diphosphate is used…

Biological Physics · Physics 2017-01-31 David W. L. Hukins

Adenosine-5'-triphosphate (ATP) plays a crucial role in many biocatalytic reactions and its regeneration can influence the performance of a related enzymatic reaction significantly. Here, we established an electrochemically coupled ATP…

Biomolecules · Quantitative Biology 2023-07-13 Regine Siedentop , Tobias Prenzel , Siegfried R. Waldvogel , Katrin Rosenthal , Stephan Lütz

The motility of cilia and flagella is driven by thousands of dynein motors that hydrolyze adenosine triphosphate (ATP). Despite decades of genetic, biochemical, structural and biophysical studies, some aspects of ciliary motility remain…

Biological Physics · Physics 2015-12-22 Daniel T. N. Chen , Michael Heymann , Seth Fraden , Daniela Nicastro , Zvonimir Dogic

The cytoskeletal component actomyosin is a canonical example of active matter since the powerstroke cycle locally converts chemical energy in the form of adenoside triphosphate (ATP) into mechanical work for remodelling. Observing myosin II…

Biological Physics · Physics 2024-12-05 Sami C. Al-Izzi , Sedigheh Ghanbarzadeh Nodehi , Darius V. Köster , Richard G. Morris

We present a novel approach for benchmarking and validating quantitative phase tomography (QPT) systems using three-dimensional microphantoms. These microphantoms, crafted from biological and imaging data, replicate the optical and…

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