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Background: Trace quantities of contaminating DNA are widespread in the laboratory environment, but their presence has received little attention in the context of high throughput sequencing. This issue is highlighted by recent works that…

Genomics · Quantitative Biology 2015-06-18 Richard W Lusk

Previous theoretical studies have shown that measuring the transverse current across DNA strands while they translocate through a nanopore or channel may provide a statistically distinguishable signature of the DNA bases, and may thus allow…

Soft Condensed Matter · Physics 2009-11-21 Matt Krems , Michael Zwolak , Yuriy V. Pershin , Massimiliano Di Ventra

Due to the stochastic nature of biochemical processes, the copy number of any given type of molecule inside a living cell often exhibits large temporal fluctuations. Here, we develop analytic methods to investigate how the noise arising…

Subcellular Processes · Quantitative Biology 2015-05-13 Li-ping Xiong , Yu-qiang Ma , Lei-Han Tang

Noise in the expression of a gene produces fluctuations in the concentration of the gene product. These fluctuations can interfere with optimal function or can be exploited to generate beneficial diversity between cells; gene expression…

Molecular Networks · Quantitative Biology 2015-05-13 Namiko Mitarai , Ian B. Dodd , Michael T. Crooks , Kim Sneppen

Noise in gene expression, either due to inherent stochasticity or to varying inter- and intracellular environment, can generate significant cell-to-cell variability of protein levels in clonal populations. We present a theoretical…

Other Quantitative Biology · Quantitative Biology 2010-04-08 Julia Rausenberger , Christian Fleck , Jens Timmer , Markus Kollmann

Individual cells exhibit substantial heterogeneity in protein abundance and activity, which is frequently reflected in broad distributions of fluorescently labeled reporters. Since all cellular components are intrinsically fluorescent to…

Quantitative Methods · Quantitative Biology 2023-06-13 Gabriel Torregrosa , David Oriola , Vikas Trivedi , Jordi Garcia-Ojalvo

Changes in a cell's external or internal conditions are usually reflected in the concentrations of the relevant transcription factors. These proteins in turn modulate the expression levels of the genes under their control and sometimes need…

Molecular Networks · Quantitative Biology 2013-08-01 Gasper Tkacik , Curtis G Callan , William Bialek

When cells measure concentrations of chemical signals, they may average multiple measurements over time in order to reduce noise in their measurements. However, when cells are in a environment that changes over time, past measurements may…

Cell Behavior · Quantitative Biology 2025-03-05 Aparajita Kashyap , Wei Wang , Brian A. Camley

Living cells provide a fluctuating, out-of-equilibrium environment in which genes must coordinate cellular function. DNA looping, which is a common means of regulating transcription, is very much a stochastic process; the loops arise from…

Biomolecules · Quantitative Biology 2015-05-18 Yih-Fan Chen , J. N. Milstein , Jens-Christian Meiners

Cellular decision making is based on regulatory circuits that associate signal thresholds to specific physiological actions. This transmission of information is subjected to molecular noise what can decrease its fidelity. Here, we show…

Molecular Networks · Quantitative Biology 2017-02-08 Guillermo Rodrigo , Juan F. Poyatos

We show how one may analytically compute the stationary density of the distribution of molecular constituents in populations of cells in the presence of noise arising from either bursting transcription or translation, or noise in…

Molecular Networks · Quantitative Biology 2015-10-15 Michael C. Mackey , Marta Tyran-Kamińska , Romain Yvinec

Inside individual cells, expression of genes is inherently stochastic and manifests as cell-to-cell variability or noise in protein copy numbers. Since proteins half-lives can be comparable to the cell-cycle length, randomness in…

Molecular Networks · Quantitative Biology 2015-10-06 Mohammad Soltani , Cesar Augusto Vargas-Garcia , Duarte Antunes , Abhyudai Singh

Biomolecular condensates have been shown to play a fundamental role in localizing biochemistry in a cell. RNA is a common constituent of condensates, and can determine their biophysical properties. Functions of biomolecular condensates are…

Subcellular Processes · Quantitative Biology 2023-02-22 Alex Mayer , Grace McLaughlin , Sierra Cole , Amy Gladfelter , Marcus Roper

Cellular biology exists embedded in a world dominated by random dynamics and chance. Many vital molecules and pieces of cellular machinery diffuse within cells, moving along random trajectories as they collide with the other biomolecular…

Cell Behavior · Quantitative Biology 2012-08-13 Iain G. Johnston

Cells are constantly exposed to fluctuating environmental conditions. External signals are sensed, processed and integrated by cellular signal transduction networks, which translate input signals into specific cellular responses by means of…

Molecular Networks · Quantitative Biology 2012-05-29 Pau Rué , Núria Domedel-Puig , Jordi Garcia-Ojalvo , Antonio J. Pons

Exploiting the information provided by the molecular noise of a biological process has proven to be valuable in extracting knowledge about the underlying kinetic parameters and sources of variability from single cell measurements. However,…

Quantitative Methods · Quantitative Biology 2013-08-30 Jakob Ruess , Andreas Milias-Argeitis , John Lygeros

We study by simulation the effect of the diffusive motion of repressor molecules on the noise in mRNA and protein levels in the case of a repressed gene. We find that spatial fluctuations due to diffusion can drastically enhance the noise…

Molecular Networks · Quantitative Biology 2007-05-23 Jeroen S. van Zon , Marco J. Morelli , Sorin Tanase-Nicola , Pieter Rein ten Wolde

A general theory is presented for the photodetection statistics of coherent radiation that has been amplified by a disordered medium. The beating of the coherent radiation with the spontaneous emission increases the noise above the…

Quantum Physics · Physics 2007-05-23 M. Patra , C. W. J. Beenakker

Regulation of intrinsic noise in gene expression is essential for many cellular functions. Correspondingly, there is considerable interest in understanding how different molecular mechanisms of gene expression impact variations in protein…

Molecular Networks · Quantitative Biology 2011-03-02 Tao Jia , Rahul V. Kulkarni

A key goal of systems biology is the predictive mathematical description of gene regulatory circuits. Different approaches are used such as deterministic and stochastic models, models that describe cell growth and division explicitly or…

Molecular Networks · Quantitative Biology 2012-10-12 Rahul Marathe , Veronika Bierbaum , David Gomez , Stefan Klumpp
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