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The development of single-cell and spatial transcriptomics has revolutionized our capacity to investigate cellular properties, functions, and interactions in both cellular and spatial contexts. However, the analysis of single-cell and…

Genomics · Quantitative Biology 2024-12-09 Shuang Ge , Shuqing Sun , Huan Xu , Qiang Cheng , Zhixiang Ren

Spatially resolved transcriptomics represents a significant advancement in single-cell analysis by offering both gene expression data and their corresponding physical locations. However, this high degree of spatial resolution entails a…

Genomics · Quantitative Biology 2024-03-19 Xiaoyu Li , Wenwen Min , Shunfang Wang , Changmiao Wang , Taosheng Xu

Single-cell RNA-sequencing technologies may provide valuable insights to the understanding of the composition of different cell types and their functions within a tissue. Recent technologies such as spatial transcriptomics, enable the…

Applications · Statistics 2023-05-16 Arhit Chakrabarti , Yang Ni , Bani K. Mallick

Human and animal tissues consist of heterogeneous cell types that organize and interact in highly structured manners. Bulk and single-cell sequencing technologies remove cells from their original microenvironments, resulting in a loss of…

Quantitative Methods · Quantitative Biology 2022-02-08 Boxiang Liu , Yanjun Li , Liang Zhang

In order to understand the complexities of cellular biology, researchers are interested in two important metrics: the genetic expression information of cells and their spatial coordinates within a tissue sample. However, state-of-the art…

Machine Learning · Computer Science 2023-11-02 J. Ding , S. N. Zaman , P. Y. Chen , D. Wang

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

Single-cell-resolution spatial transcriptomics profiles gene expression at cellular locations in native tissues, yet accurate cell-type annotation remains challenging: imaging-based platforms are constrained by targeted gene panels, whereas…

Cell Behavior · Quantitative Biology 2026-05-27 Yiyang Zhang , Bokai Zhao , Xiaoru Zhang , Zongchang Du , Xiaojuan Sun , Tianzi Jiang

Spatial Transcriptomics enables mapping of gene expression within its native tissue context, but current platforms measure only a limited set of genes due to experimental constraints and excessive costs. To overcome this, computational…

Genomics · Quantitative Biology 2025-11-20 Amit Kumar , Maninder Kaur , Raghvendra Mall , Sukrit Gupta

The integration of single-cell proteomic data is often hindered by the fragmented nature of targeted antibody panels. To address this limitation, we introduce scpFormer, a transformer-based foundation model designed for single-cell…

Quantitative Methods · Quantitative Biology 2026-04-23 Qifeng Zhou , Lei Yu , Yuzhi Guo , Yuwei Miao , Hehuan Ma , Wenliang Zhong , Lin Xu , Junzhou Huang

Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

Missing data is a pervasive issue in both scientific and engineering tasks, especially for the modeling of spatiotemporal data. This problem attracts many studies to contribute to data-driven solutions. Existing imputation solutions mainly…

Machine Learning · Computer Science 2024-07-26 Tong Nie , Guoyang Qin , Wei Ma , Yuewen Mei , Jian Sun

Spatial studies of transcriptome provide biologists with gene expression maps of heterogeneous and complex tissues. However, most experimental protocols for spatial transcriptomics suffer from the need to select beforehand a small fraction…

Machine Learning · Computer Science 2019-05-08 Romain Lopez , Achille Nazaret , Maxime Langevin , Jules Samaran , Jeffrey Regier , Michael I. Jordan , Nir Yosef

Spatial transcriptomics data analysis integrates cellular transcriptional activity with spatial coordinates to identify spatial domains, infer cell-type dynamics, and characterize gene expression patterns within tissues. Despite recent…

Quantitative Methods · Quantitative Biology 2026-03-25 Sean Cottrell , Guo-Wei Wei , Longxiu Huang

Spatial Transcriptomics (ST) technologies provide biologists with rich insights into single-cell biology by preserving spatial context of cells. Building foundational models for ST can significantly enhance the analysis of vast and complex…

Genomics · Quantitative Biology 2025-07-24 Suyuan Zhao , Yizhen Luo , Ganbo Yang , Yan Zhong , Hao Zhou , Zaiqing Nie

Spatial transcriptomics (ST) has revolutionized biomedical research by enabling high resolution gene expression profiling within tissues. However, the high cost and scarcity of high resolution ST data remain significant challenges. We…

Computer Vision and Pattern Recognition · Computer Science 2025-07-24 Yaoyu Fang , Jiahe Qian , Xinkun Wang , Lee A. Cooper , Bo Zhou

Single-cell RNA sequencing (scRNA-seq) data exhibit strong and reproducible statistical structure. This has motivated the development of large-scale foundation models, such as TranscriptFormer, that use transformer-based architectures to…

Genomics · Quantitative Biology 2026-02-19 Huan Souza , Pankaj Mehta

Spatial Transcriptomics is a novel technology that aligns histology images with spatially resolved gene expression profiles. Although groundbreaking, it struggles with gene capture yielding high corruption in acquired data. Given potential…

Computer Vision and Pattern Recognition · Computer Science 2024-09-30 Gabriel Mejia , Daniela Ruiz , Paula Cárdenas , Leonardo Manrique , Daniela Vega , Pablo Arbeláez

Celcomen leverages a mathematical causality framework to disentangle intra- and inter- cellular gene regulation programs in spatial transcriptomics and single-cell data through a generative graph neural network. It can learn gene-gene…

Deep learning-based nuclei segmentation and classification in pathology images typically rely on large-scale pixel-level manual annotations, which are costly and difficult to obtain across diverse tissues and staining conditions. To address…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Kazuya Nishimura , Ryoma Bise , Haruka Hirose , Yasuhiro Kojima

Spatial transcriptomics methods capture cellular measurements such as gene expression and cell types at specific locations in a cell, helping provide a localized picture of tissue health. Traditional visualization techniques superimpose the…

Quantitative Methods · Quantitative Biology 2024-10-16 Siyuan Zhao , G. Elisabeta Marai
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