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Single particle cryo-electron microscopy is a vital tool for 3D characterization of protein structures. A typical workflow involves acquiring projection images of a collection of randomly oriented particles, picking and classifying…

Image and Video Processing · Electrical Eng. & Systems 2021-03-22 S. V. Venkatakrishnan , Puneet Juneja , Hugh O'Neill

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

We present a unified framework tackling two problems: class-specific 3D reconstruction from a single image, and generation of new 3D shape samples. These tasks have received considerable attention recently; however, most existing approaches…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Paul Henderson , Vittorio Ferrari

The development of X-ray Free Electron Lasers (XFELs) has opened numerous opportunities to probe atomic structure and ultrafast dynamics of various materials. Single Particle Imaging (SPI) with XFELs enables the investigation of biological…

Computer Vision and Pattern Recognition · Computer Science 2023-11-29 Zhantao Chen , Cong Wang , Mingye Gao , Chun Hong Yoon , Jana B. Thayer , Joshua J. Turner

Single particle cryo-electron microscopy has become a critical tool in structural biology over the last decade, able to achieve atomic scale resolution in three dimensional models from hundreds of thousands of (noisy) two-dimensional…

Numerical Analysis · Mathematics 2023-07-19 Aaditya V. Rangan , Leslie Greengard

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

Single image 3D reconstruction is an important but challenging task that requires extensive knowledge of our natural world. Many existing methods solve this problem by optimizing a neural radiance field under the guidance of 2D diffusion…

Computer Vision and Pattern Recognition · Computer Science 2023-06-30 Minghua Liu , Chao Xu , Haian Jin , Linghao Chen , Mukund Varma T , Zexiang Xu , Hao Su

A robust single-shot 3D shape reconstruction technique integrating the fringe projection profilometry (FPP) technique with the deep convolutional neural networks (CNNs) is proposed in this letter. The input of the proposed technique is a…

Image and Video Processing · Electrical Eng. & Systems 2019-09-18 Hieu Nguyen , Hui Li , Qiang Qiu , Yuzeng Wang , Zhaoyang Wang

Three-dimensional particle reconstruction with limited two-dimensional projections is an under-determined inverse problem that the exact solution is often difficult to be obtained. In general, approximate solutions can be obtained by…

Image and Video Processing · Electrical Eng. & Systems 2021-09-14 Qi Gao , Shaowu Pan , Hongping Wang , Runjie Wei , Jinjun Wang

We propose an approach to 3D reconstruction via inverse procedural modeling and investigate two variants of this approach. The first option consists in the fitting set of input parameters using a genetic algorithm. We demonstrate the…

Graphics · Computer Science 2023-10-23 Albert Garifullin , Nikolay Maiorov , Vladimir Frolov

X-ray single particle imaging involves the measurement of a large number of noisy diffraction patterns of isolated objects in random orientations. The missing information about these patterns is then computationally recovered in order to…

Image and Video Processing · Electrical Eng. & Systems 2020-05-25 Kartik Ayyer

We introduce Intrinsic Image Fusion, a method that reconstructs high-quality physically based materials from multi-view images. Material reconstruction is highly underconstrained and typically relies on analysis-by-synthesis, which requires…

Computer Vision and Pattern Recognition · Computer Science 2026-03-24 Peter Kocsis , Lukas Höllein , Matthias Nießner

We introduce the EMC algorithm for reconstructing a particle's 3D diffraction intensity from very many photon shot-noise limited 2D measurements, when the particle orientation in each measurement is unknown. The algorithm combines a…

Data Analysis, Statistics and Probability · Physics 2010-03-04 Duane Ne-Te Loh , Veit Elser

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Magnetic Resonance Imaging (MRI) is a powerful imaging technique widely used for visualizing structures within the human body and in other fields such as plant sciences. However, there is a demand to develop fast 3D-MRI reconstruction…

Image and Video Processing · Electrical Eng. & Systems 2025-12-09 Arya Bangun , Zhuo Cao , Alessio Quercia , Hanno Scharr , Elisabeth Pfaehler

With the rapid development of computer graphics and vision, several three-dimensional (3D) reconstruction techniques have been proposed and used to obtain the 3D representation of objects in the form of point cloud models, mesh models, and…

Computer Vision and Pattern Recognition · Computer Science 2021-12-02 Rui Wang

Available super-resolution techniques for 3D images are either computationally inefficient prior-knowledge-based iterative techniques or deep learning methods which require a large database of known low- and high-resolution image pairs. A…

Computer Vision and Pattern Recognition · Computer Science 2024-10-30 Janka Hatvani , Adrian Basarab , Jean-Yves Tourneret , Miklós Gyöngy , Denis Kouamé

Single-particle cryo-electron microscopy (cryo-EM) reconstructs the three-dimensional (3D) structure of bio-molecules from a large set of 2D projection images with random and unknown orientations. A crucial step in the single-particle…

Image and Video Processing · Electrical Eng. & Systems 2020-06-24 Mona Zehni , Laurène Donati , Emmanuel Soubies , Zhizhen J. Zhao , Michael Unser

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the task of image restoration can be decomposed into two interlinked inverse problems: firstly, the characterization of the Point Spread Function (PSF) and…

Image and Video Processing · Electrical Eng. & Systems 2024-11-08 Julien Ajdenbaum , Emilie Chouzenoux , Claire Lefort , Ségolène Martin , Jean-Christophe Pesquet
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