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Fluorescence microscopy has become a widely used tool for studying various biological structures of in vivo tissue or cells. However, quantitative analysis of these biological structures remains a challenge due to their complexity which is…

Computer Vision and Pattern Recognition · Computer Science 2019-04-16 Soonam Lee , Chichen Fu , Paul Salama , Kenneth W. Dunn , Edward J. Delp

Generally, microscopy image analysis in biology relies on the segmentation of individual nuclei, using a dedicated stained image, to identify individual cells. However stained nuclei have drawbacks like the need for sample preparation, and…

Image and Video Processing · Electrical Eng. & Systems 2022-11-08 Gendarme Mathieu , Lambert Annika M. , El Debs Bachir

We have developed an automatic method for segmenting fluorescence lifetime (FLT) imaging microscopy (FLIM) images of cells inspired by a multi-resolution community detection (MCD) based network segmentation method. The image processing…

Medical Physics · Physics 2014-01-06 Dandan Hu , Pinaki Sarder , Peter Ronhovde , Sandra Orthaus , Samuel Achilefu , Zohar Nussinov

Deep learning provides us with powerful methods to perform nucleus or cell segmentation with unprecedented quality. However, these methods usually require large training sets of manually annotated images, which are tedious and expensive to…

Image and Video Processing · Electrical Eng. & Systems 2023-01-11 Thomas Bonte , Maxence Philbert , Emeline Coleno , Edouard Bertrand , Arthur Imbert , Thomas Walter

Separating and labeling each instance of a nucleus (instance-aware segmentation) is the key challenge in segmenting single cell nuclei on fluorescence microscopy images. Deep Neural Networks can learn the implicit transformation of a…

Computer Vision and Pattern Recognition · Computer Science 2021-08-11 Florian Kromp , Lukas Fischer , Eva Bozsaky , Inge Ambros , Wolfgang Doerr , Sabine Taschner-Mandl , Peter Ambros , Allan Hanbury

Determination of fundamental mechanisms of disease often hinges on histopathology visualization and quantitative image analysis. Currently, the analysis of multi-channel fluorescence tissue images is primarily achieved by manual…

Purpose Automated segmentation of anatomical structures in medical image analysis is a prerequisite for autonomous diagnosis as well as various computer and robot aided interventions. Recent methods based on deep convolutional neural…

Computer Vision and Pattern Recognition · Computer Science 2020-09-22 Max-Heinrich Laves , Jens Bicker , Lüder A. Kahrs , Tobias Ortmaier

Deep learning techniques have successfully been employed in numerous computer vision tasks including image segmentation. The techniques have also been applied to medical image segmentation, one of the most critical tasks in computer-aided…

Computer Vision and Pattern Recognition · Computer Science 2020-06-30 Titinunt Kitrungrotsakul , Iwamoto Yutaro , Lanfen Lin , Ruofeng Tong , Jingsong Li , Yen-Wei Chen

Cell segmentation is essential in biomedical research for analyzing cellular morphology and behavior. Deep learning methods, particularly convolutional neural networks (CNNs), have revolutionized cell segmentation by extracting intricate…

Image and Video Processing · Electrical Eng. & Systems 2024-08-27 Boyuan Peng , Jiaju Chen , P. Bilha Githinji , Ijaz Gul , Qihui Ye , Minjiang Chen , Peiwu Qin , Xingru Huang , Chenggang Yan , Dongmei Yu , Jiansong Ji , Zhenglin Chen

AI-assisted nuclei segmentation in histopathological images is a crucial task in the diagnosis and treatment of cancer diseases. It decreases the time required to manually screen microscopic tissue images and can resolve the conflict…

Image and Video Processing · Electrical Eng. & Systems 2023-11-21 Hesham Ali , Idriss Tondji , Mennatullah Siam

Accurate segmentation and precise morphological analysis of neuronal cells in fluorescence microscopy images are crucial steps in neuroscience and biomedical imaging applications. However, this process is labor-intensive and time-consuming,…

Image and Video Processing · Electrical Eng. & Systems 2025-10-23 Banan Alnemri , Arwa Basbrain

Medical image segmentation is vital for modern healthcare and is a key element of computer-aided diagnosis. While recent advancements in computer vision have explored unsupervised segmentation using pre-trained models, these methods have…

Computer Vision and Pattern Recognition · Computer Science 2025-08-07 Mosong Ma , Tania Stathaki , Michalis Lazarou

Cell instance segmentation in fluorescence microscopy images is becoming essential for cancer dynamics and prognosis. Data extracted from cancer dynamics allows to understand and accurately model different metabolic processes such as…

Current methods for developing foundation models in medical image segmentation rely on two primary assumptions: a fixed set of classes and the immediate availability of a substantial and diverse training dataset. However, this can be…

Computer Vision and Pattern Recognition · Computer Science 2024-05-28 Xiaoyang Chen , Hao Zheng , Yifang Xie , Yuncong Ma , Tengfei Li

For complex segmentation tasks, fully automatic systems are inherently limited in their achievable accuracy for extracting relevant objects. Especially in cases where only few data sets need to be processed for a highly accurate result,…

Computer Vision and Pattern Recognition · Computer Science 2017-09-12 Mario Amrehn , Sven Gaube , Mathias Unberath , Frank Schebesch , Tim Horz , Maddalena Strumia , Stefan Steidl , Markus Kowarschik , Andreas Maier

Skin lesion segmentation is one of the crucial steps for an efficient non-invasive computer-aided early diagnosis of melanoma. This paper investigates how color information, besides saliency, can be used to determine the pigmented lesion…

Image and Video Processing · Electrical Eng. & Systems 2021-11-08 Giuliana Ramella

Fluorescence microscopy images contain several channels, each indicating a marker staining the sample. Since many different marker combinations are utilized in practice, it has been challenging to apply deep learning based segmentation…

Computer Vision and Pattern Recognition · Computer Science 2021-01-28 Alvaro Gomariz , Raphael Egli , Tiziano Portenier , César Nombela-Arrieta , Orcun Goksel

Nuclei segmentation is a fundamental but challenging task in the quantitative analysis of histopathology images. Although fully-supervised deep learning-based methods have made significant progress, a large number of labeled images are…

Image and Video Processing · Electrical Eng. & Systems 2024-01-22 Xinyi Yu , Guanbin Li , Wei Lou , Siqi Liu , Xiang Wan , Yan Chen , Haofeng Li

We introduce CellSegmenter, a structured deep generative model and an amortized inference framework for unsupervised representation learning and instance segmentation tasks. The proposed inference algorithm is convolutional and…

Computer Vision and Pattern Recognition · Computer Science 2020-11-26 Luca D'Alessio , Mehrtash Babadi

Primary neuronal cultures have been widely used to study neuronal morphology, neurophysiology, neurodegenerative processes, and molecular mechanism of synaptic plasticity underlying learning and memory. Yet, the unique behavioral properties…

Image and Video Processing · Electrical Eng. & Systems 2020-08-04 Mikhail E. Kandel , Eunjae Kim , Young Jae Lee , Gregory Tracy , Hee Jung Chung , Gabriel Popescu
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