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We use Topological Data Analysis tools for studying the inner organization of cells in segmented images of epithelial tissues. More specifically, for each segmented image, we compute different persistence barcodes, which codify lifetime of…

Computer Vision and Pattern Recognition · Computer Science 2022-04-11 N. Atienza , M. J. Jimenez , M. Soriano-Trigueros

Spatial transcriptomics (ST) enables transcriptome-wide profiling while preserving the spatial context of tissues, offering unprecedented opportunities to study tissue organization and cell-cell interactions in situ. Despite recent…

Computer Vision and Pattern Recognition · Computer Science 2026-01-21 Wei Wang , Quoc-Toan Ly , Chong Yu , Jun Bai

Spatial variable genes (SVGs) reveal critical information about tissue architecture, cellular interactions, and disease microenvironments. As spatial transcriptomics (ST) technologies proliferate, accurately identifying SVGs across diverse…

Applications · Statistics 2025-10-21 Jiawen Chen , Jinwei Zhang , Dongshen Peng , Yutong Song , Aitong Ruan , Yun Li , Didong Li

The advent of next-generation sequencing-based spatially resolved transcriptomics (SRT) techniques has reshaped genomic studies by enabling high-throughput gene expression profiling while preserving spatial and morphological context.…

Applications · Statistics 2023-12-14 Bencong Zhu , Guanyu Hu , Yang Xie , Lin Xu , Xiaodan Fan , Qiwei Li

Deep learning methods are widely applied in digital pathology to address clinical challenges such as prognosis and diagnosis. As one of the most recent applications, deep models have also been used to extract molecular features from whole…

Image and Video Processing · Electrical Eng. & Systems 2022-03-29 Amir Safarpoor , Jason D. Hipp , H. R. Tizhoosh

Intrathoracic airway segmentation in computed tomography (CT) is a prerequisite for various respiratory disease analyses such as chronic obstructive pulmonary disease (COPD), asthma and lung cancer. Unlike other organs with simpler shapes…

Image and Video Processing · Electrical Eng. & Systems 2023-06-16 Puyang Wang , Dazhou Guo , Dandan Zheng , Minghui Zhang , Haogang Yu , Xin Sun , Jia Ge , Yun Gu , Le Lu , Xianghua Ye , Dakai Jin

Automatic integration of whole slide images (WSIs) and gene expression profiles has demonstrated substantial potential in precision clinical diagnosis and cancer progression studies. However, most existing studies focus on individual gene…

Computer Vision and Pattern Recognition · Computer Science 2026-01-14 Junzhuo Liu , Xuemei Du , Daniel Reisenbuchler , Ye Chen , Markus Eckstein , Christian Matek , Friedrich Feuerhake , Dorit Merhof

While spatial transcriptomics (ST) has advanced our understanding of gene expression in tissue context, its high experimental cost limits its large-scale application. Predicting ST from pathology images is a promising, cost-effective…

Computer Vision and Pattern Recognition · Computer Science 2026-03-25 Zhiceng Shi , Changmiao Wang , Jun Wan , Wenwen Min

This paper introduces a topological framework for interpreting the internal representations of Multilayer Perceptrons (MLPs). We construct a simplicial tower, a sequence of simplicial complexes connected by simplicial maps, that captures…

Machine Learning · Computer Science 2025-06-03 Eduardo Paluzo-Hidalgo

Understanding how the spatial structure of blood vessel networks relates to their function in healthy and abnormal biological tissues could improve diagnosis and treatment for diseases such as cancer. New imaging techniques can generate…

Quantitative Methods · Quantitative Biology 2019-07-23 Helen M Byrne , Heather A Harrington , Ruth Muschel , Gesine Reinert , Bernadette J Stolz , Ulrike Tillmann

Characterization of topology and dimensionality of spectral feature spaces provides insight into information content. The objective of this study is to characterize topology and spectral dimensionality of spectral mixing spaces representing…

Geophysics · Physics 2023-07-11 Christopher Small , Daniel Sousa

Single-cell RNA sequencing (scRNA-seq) and spatially-resolved imaging/sequencing technologies have revolutionized biomedical research. On one hand, scRNA-seq provides information about a large portion of the transcriptome for individual…

Computational Engineering, Finance, and Science · Computer Science 2026-03-24 Arezou Rahimi , Luis A. Vale-Silva , Maria Faelth Savitski , Jovan Tanevski , Julio Saez-Rodriguez

Multiplexed imaging data are revolutionizing our understanding of the composition and organization of tissues and tumors. A critical aspect of such tissue profiling is quantifying the spatial relationship relationships among cells at…

Quantitative Methods · Quantitative Biology 2024-05-06 Ajit J. Nirmal , Peter K. Sorger

The Topological Signal Processing (TSP) framework has been recently developed to analyze signals defined over simplicial complexes, i.e. topological spaces represented by finite sets of elements that are closed under inclusion of subsets…

Signal Processing · Electrical Eng. & Systems 2021-12-14 Stefania Sardellitti , Sergio Barbarossa , Lucia Testa

Microscopic examination of slides prepared from tissue samples is the primary tool for detecting and classifying cancerous lesions, a process that is time-consuming and requires the expertise of experienced pathologists. Recent advances in…

Computer Vision and Pattern Recognition · Computer Science 2024-10-15 Saba Fatema , Brighton Nuwagira , Sayoni Chakraborty , Reyhan Gedik , Baris Coskunuzer

Landmark detection is a critical component of the image processing pipeline for automated aortic size measurements. Given that the thoracic aorta has a relatively conserved topology across the population and that a human annotator with…

Image and Video Processing · Electrical Eng. & Systems 2023-04-18 Zhangxing Bian , Jiayang Zhong , Yanglong Lu , Charles R. Hatt , Nicholas S. Burris

Developing clinically useful cell-level analysis tools in digital pathology remains challenging due to limitations in dataset granularity, inconsistent annotations, high computational demands, and difficulties integrating new technologies…

Computer Vision and Pattern Recognition · Computer Science 2025-12-02 Nikita Shvetsov , Thomas K. Kilvaer , Masoud Tafavvoghi , Anders Sildnes , Kajsa Møllersen , Lill-Tove Rasmussen Busund , Lars Ailo Bongo

Many biological phenomena are inherently multiscale, i.e. they are characterized by interactions involving different spatial and temporal scales simultaneously. Though several approaches have been proposed to provide "multilayer" models,…

Computational Engineering, Finance, and Science · Computer Science 2010-11-03 Diletta Cacciagrano , Flavio Corradini , Emanuela Merelli , Luca Tesei

Accurate automatic organ segmentation is an important yet challenging problem for medical image analysis. The pancreas is an abdominal organ with very high anatomical variability. This inhibits traditional segmentation methods from…

Computer Vision and Pattern Recognition · Computer Science 2016-06-28 Holger R. Roth , Le Lu , Amal Farag , Andrew Sohn , Ronald M. Summers

Unlike other tissue types, like epithelial tissue, which consist of cells with a much more homogeneous structure and function, the nervous tissue spans in a complex multilayer environment whose topographical features display a large…

Biological Physics · Physics 2017-05-12 C. Simitzi , A. Ranella , E. Stratakis