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The volume occupied by unconstrained bacterial DNA in physiological solutions exceeds 1000 times the volume of the cell. Still, it is confined to a well defined region of the cell called the nucleoid, which occupies only a fraction of the…

Biological Physics · Physics 2016-09-27 Marc Joyeux

The behavior of long DNA molecules in a cell-sized confined space was investigated. We prepared water-in-oil droplets covered by phospholipids, which mimic the inner space of a cell, following the encapsulation of DNA molecules with…

Biological Physics · Physics 2015-04-14 T. Hamada , R. Fujimoto , S. F. Shimobayashi , M. Ichikawa , M. Takagi

Liquid-liquid phase separation is key to understanding aqueous two-phase systems (ATPS) arising throughout cell biology, medical science, and the pharmaceutical industry. Controlling the detailed morphology of phase-separating compound…

Soft Condensed Matter · Physics 2023-10-27 Eric W. Hester , Sean P. Carney , Vishwesh Shah , Alyssa Arnheim , Bena Patel , Dino Di Carlo , Andrea L. Bertozzi

We use a coarse-grained molecular model to study the self-assembly process of complexes of cationic and neutral lipids with DNA molecules ("lipoplexes") - a promising nonviral carrier of DNA for gene therapy. We identify the resulting…

Soft Condensed Matter · Physics 2011-05-31 Oded Farago , Niels Grønbech-Jensen

Brownian motion provides access to hydrodynamic properties of nanoscale objects independent of their optical resolvability. Here, we present a diffusion-based approach to infer effective particle size distributions of DNA-functionalized…

We show that a long DNA molecule can form a novel condensed phase of matter, the pseudoscalar liquid crystal, that consists of aperiodically ordered DNA fragments in right-handed B and left-handed Z forms. We discuss the possibility of…

Soft Condensed Matter · Physics 2009-05-14 K. G. Petrosyan , Chin-Kun Hu

We study the problem of the self-assembly of nanoparticles (NPs) into finite mesoscopic structures with a programmed local morphology and complex overall shape. Our proposed building blocks are NPs directionally-functionalized with DNA. The…

Soft Condensed Matter · Physics 2013-11-04 Jonathan D. Halverson , Alexei V. Tkachenko

Morphological transitions of phase separation associated with the asymmetry of lipid composition were investigated using micrometer-sized vesicles of lipid bilayers made from a lipid mixture. The complete macro-phase-separated morphology…

Biological Physics · Physics 2015-04-14 Shunsuke F. Shimobayashi , Masatoshi Ichikawa , Takashi Taniguchi

Disordered hyperuniformity (DHU) is a recently discovered novel state of many-body systems that is characterized by vanishing normalized infinite-wavelength density fluctuations similar to a perfect crystal, yet possesses an amorphous…

Soft Condensed Matter · Physics 2021-10-27 Yang Jiao

Phase separation under non-equilibrium conditions is exploited by biological cells to organize their cytoplasm but remains poorly understood as a physical phenomenon. Here, we study a ternary fluid model in which phase-separating molecules…

Soft Condensed Matter · Physics 2018-02-21 Jean David Wurtz , Chiu Fan Lee

Recent experiments have been able to visualise chromosome organization in fast-growing E.coli cells. However, the mechanism underlying the spatio-temporal organization remains poorly understood. We propose that the DNA adopts a specific…

Soft Condensed Matter · Physics 2026-04-06 Shreerang Pande , Debarshi Mitra , Apratim Chatterji

Disordered hyperuniform many-body systems are distinguishable states of matter that lie between a crystal and liquid: they are like perfect crystals in the way they suppress large-scale density fluctuations and yet are like liquids or…

Soft Condensed Matter · Physics 2016-09-21 Salvatore Torquato

We investigate the dynamics of nanoscale droplets in the vicinity of chemical steps which separate parts of a substrate with different wettabilities. Due to long-ranged dispersion forces, nanodroplets positioned on one side of the step…

Soft Condensed Matter · Physics 2009-09-29 A. Moosavi , M. Rauscher , S. Dietrich

Concentrated solutions of short blunt-ended DNA duplexes, down to 6 base pairs, are known to order into the nematic liquid crystal phase. This self-assembly is due to the stacking interactions between the duplex terminals that promotes…

Soft Condensed Matter · Physics 2015-05-30 Cristiano De Michele , Tommaso Bellini , Francesco Sciortino

In the demanding biosensing environment, improving selection efficiency strategies has become an issue of great significance. DNA minicircles containing between 200 and 400 base-pairs, also named microDNA, are representative of the…

Biological Physics · Physics 2018-05-14 François Sicard , Nicolas Destainville , Philippe Rousseau , Catherine Tardin , Manoel Manghi

Disordered hyperuniform structures are an exotic state of matter having suppressed density fluctuations at large length-scale similar to perfect crystals and quasicrystals but without any long range orientational order. In the past decade,…

Soft Condensed Matter · Physics 2024-11-18 Yusheng Lei , Ran Ni

DNA-functionalized particles have great potential for the design of complex self-assembled materials. The major hurdle in realizing crystal structures from DNA-functionalized particles is expected to be kinetic barriers that trap the system…

Soft Condensed Matter · Physics 2015-03-06 Yajun Ding , Jeetain Mittal

Living cells use phase separation and concentration gradients to organize chemical compartments in space. Here, we present a theoretical study of droplet dynamics in gradient systems. We derive the corresponding growth law of droplets and…

Biological Physics · Physics 2017-05-19 Christoph A. Weber , Chiu Fan Lee , Frank Jülicher

Under appropriate conditions, mixtures of cationic and neutral lipids and DNA in water condense into complexes in which DNA strands form local 2D smectic lattices intercalated between lipid bilayer membranes in a lamellar stack. These…

Soft Condensed Matter · Physics 2009-10-31 L. Golubovic , T. C. Lubensky , C. S. O'Hern

The folding of RNA and DNA strands plays crucial roles in biological systems and bionanotechnology. However, studying these processes with high-resolution numerical models is beyond current computational capabilities due to the timescales…

Soft Condensed Matter · Physics 2024-02-07 F. Tosti Guerra , E. Poppleton , P. Šulc , L. Rovigatti