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Gene expression and regulation rely on an apparently finely tuned set of reactions between some proteins and DNA. Such DNA-binding proteins have to find specific sequences on very long DNA molecules and they mostly do so in absence of any…

Biological Physics · Physics 2013-12-02 Maria Barbi , Fabien Paillusson

There is increasing evidence that protein binding to specific sites along DNA can activate the reading out of genetic information without coming into direct physical contact with the gene. There also is evidence that these distant but…

Subcellular Processes · Quantitative Biology 2019-12-19 William Bialek , Thomas Gregor , Gašper Tkačik

Genomic data sets are growing dramatically as the cost of sequencing continues to decline and small sequencing devices become available. Enormous community databases store and share this data with the research community, but some of these…

Recent whole genome polymerase binding assays have shown that a large proportion of unexpressed genes have pre-assembled RNA pol II transcription initiation complex stably bound to their promoters. Some such promoter proximally paused genes…

Molecular Networks · Quantitative Biology 2015-03-19 Alistair N. Boettiger , Peter L. Ralph , Steven N. Evans

The structure of DNA Binding Proteins enables a strong interaction with their specific target site on DNA. However, recent single molecule experiment reported that proteins can diffuse on DNA. This suggests that the interactions between…

Biomolecules · Quantitative Biology 2015-05-13 Vincent Dahirel , Fabien Paillusson , Marie Jardat , Maria Barbi , Jean-Marc Victor

Large-scale DNA deformation is ubiquitous in transcriptional regulation in prokaryotes and eukaryotes alike. Though much is known about how transcription factors and constellations of binding sites dictate where and how gene regulation will…

Biomolecules · Quantitative Biology 2013-10-15 Stephanie Johnson , Yi-Ju Chen , Rob Phillips

Transcription factors (TFs) are proteins that bind to specific sites on the DNA and regulate gene activity. Identifying where TF molecules bind and how much time they spend on their target sites is key for understanding transcriptional…

Molecular Networks · Quantitative Biology 2013-10-01 Nicolae Radu Zabet , Robert Foy , Boris Adryan

Genome length varies widely among organisms, from compact genomes of prokaryotes to vast and complex genomes of eukaryotes. In this study, we theoretically identify the evolutionary pressures that may have driven this divergence in genome…

Genomics · Quantitative Biology 2025-03-03 Parthasarathi Sahu , Sashikanta Barik , Koushik Ghosh , Hemachander Subramanian

We introduce a novel method to screen the promoters of a set of genes with shared biological function, against a precompiled library of motifs, and find those motifs which are statistically over-represented in the gene set. The gene sets…

Molecular Networks · Quantitative Biology 2008-11-11 Yuval Tabach , Ran Brosh , Yossi Buganim , Anat Reiner , Or Zuk , Assif Yitzhaky , Mark Koudritsky , Varda Rotter , Eytan Domany

Despite the widespread application of latent factor analysis, existing methods suffer from the following weaknesses: requiring the number of factors to be known, lack of theoretical guarantees for learning the model structure, and…

Methodology · Statistics 2023-06-06 Dale S. Kim , Qing Zhou

Gene regulatory networks are collections of genes that interact with one other and with other substances in the cell. By measuring gene expression over time using high-throughput technologies, it may be possible to reverse engineer, or…

Applications · Statistics 2011-09-08 Andrea Rau , Florence Jaffrézic , Jean-Louis Foulley , R. W. Doerge

For short DNA molecules in crowded environments, we evaluate macroscopic parameters such as the average end-to-end distance and the twist conformation by tuning the strength of the site specific confinement driven by the crowders. The…

Soft Condensed Matter · Physics 2019-06-14 Marco Zoli

Most DNA sequencing technologies are based on the shotgun paradigm: many short reads are obtained from random unknown locations in the DNA sequence. A fundamental question, studied in arXiv:1203.6233, is what read length and coverage depth…

Information Theory · Computer Science 2022-02-09 Aditya Narayan Ravi , Alireza Vahid , Ilan Shomorony

Genetic algorithms are a well-known method for tackling the problem of variable selection. As they are non-parametric and can use a large variety of fitness functions, they are well-suited as a variable selection wrapper that can be applied…

Machine Learning · Statistics 2016-04-25 Chee Chun Gan , Gerard Learmonth

Transcription factor binding to the surface of DNA regulatory regions is one of the primary causes of regulating gene expression levels. A probabilistic approach to model protein-DNA interactions at the sequence level is through Position…

Biological Physics · Physics 2015-11-18 Jacob Clifford , Christoph Adami

Our understanding of how chromosomes structurally organize and dynamically interact has been revolutionized through the lens of long-chain polymer physics. Major protein contributors to chromosome structure and dynamics are condensin and…

The problem of fast items retrieval from a fixed collection is often encountered in most computer science areas, from operating system components to databases and user interfaces. We present an approach based on hash tables that focuses on…

Neural and Evolutionary Computing · Computer Science 2020-07-17 Dan Domnita , Ciprian Oprisa

Background: In Escherichia coli, overlapping rounds of DNA replication allow the bacteria to double in faster times than the time required to copy the genome. The precise timing of initiation of DNA replication is determined by a regulatory…

Central to the functioning of a living cell is its ability to control the readout or expression of information encoded in the genome. In many cases, a single transcription factor protein activates or represses the expression of many genes.…

Molecular Networks · Quantitative Biology 2013-05-29 Aleksandra M. Walczak , Gapser Tkacik , William Bialek

Transcriptional interactions in a cell are modulated by a variety of mechanisms that prevent their representation as pure pairwise interactions between a transcription factor and its target(s). These include, among others, transcription…

Molecular Networks · Quantitative Biology 2007-05-23 Kai Wang , Ilya Nemenman , Nilanjana Banerjee , Adam Margolin , Andrea Califano