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This paper addresses the task of nuclei segmentation in high-resolution histopathological images. We propose an auto- matic end-to-end deep neural network algorithm for segmenta- tion of individual nuclei. A nucleus-boundary model is…

Computer Vision and Pattern Recognition · Computer Science 2020-03-18 Yuxin Cui , Guiying Zhang , Zhonghao Liu , Zheng Xiong , Jianjun Hu

Nuclei appear small in size, yet, in real clinical practice, the global spatial information and correlation of the color or brightness contrast between nuclei and background, have been considered a crucial component for accurate nuclei…

Image and Video Processing · Electrical Eng. & Systems 2023-07-18 Zhenqi He , Mathias Unberath , Jing Ke , Yiqing Shen

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

We propose a new and, arguably, a very simple reduction of instance segmentation to semantic segmentation. This reduction allows to train feed-forward non-recurrent deep instance segmentation systems in an end-to-end fashion using…

Computer Vision and Pattern Recognition · Computer Science 2018-07-27 Victor Kulikov , Victor Yurchenko , Victor Lempitsky

Segmentation is one of the most significant steps in image processing. Segmenting an image is a technique that makes it possible to separate a digital image into various areas based on the different characteristics of pixels in the image.…

Image and Video Processing · Electrical Eng. & Systems 2024-11-20 Sina Derakhshandeh , Ali Mahloojifar

Fine-grained classification of microscopic image data with limited samples is an open problem in computer vision and biomedical imaging. Deep learning based vision systems mostly deal with high number of low-resolution images, whereas…

Computer Vision and Pattern Recognition · Computer Science 2020-10-07 Mengran Fan , Tapabrata Chakrabort , Eric I-Chao Chang , Yan Xu , Jens Rittscher

By their very nature microscopy images of cells and tissues consist of a limited number of object types or components. In contrast to most natural scenes, the composition is known a priori. Decomposing biological images into semantically…

Computer Vision and Pattern Recognition · Computer Science 2019-09-04 Avelino Javer , Jens Rittscher

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

Nuclei segmentation is a crucial task for whole slide image analysis in digital pathology. Generally, the segmentation performance of fully-supervised learning heavily depends on the amount and quality of the annotated data. However, it is…

Image and Video Processing · Electrical Eng. & Systems 2023-08-21 Yi Lin , Zhiyong Qu , Hao Chen , Zhongke Gao , Yuexiang Li , Lili Xia , Kai Ma , Yefeng Zheng , Kwang-Ting Cheng

Volumetric cell segmentation in fluorescence microscopy images is important to study a wide variety of cellular processes. Applications range from the analysis of cancer cells to behavioral studies of cells in the embryonic stage. Like in…

Image and Video Processing · Electrical Eng. & Systems 2022-11-29 Royden Wagner , Karl Rohr

The locations of different mRNA molecules can be revealed by multiplexed in situ RNA detection. By assigning detected mRNA molecules to individual cells, it is possible to identify many different cell types in parallel. This in turn enables…

Information Theory · Computer Science 2023-12-08 Axel Andersson , Andrea Behanova , Carolina Wählby , Filip Malmberg

We present a new instance segmentation approach tailored to biological images, where instances may correspond to individual cells, organisms or plant parts. Unlike instance segmentation for user photographs or road scenes, in biological…

Computer Vision and Pattern Recognition · Computer Science 2020-04-24 Victor Kulikov , Victor Lempitsky

Multiplex Imaging (MI) enables the simultaneous visualization of multiple biological markers in separate imaging channels at subcellular resolution, providing valuable insights into cell-type heterogeneity and spatial organization. However,…

Image and Video Processing · Electrical Eng. & Systems 2024-11-07 Simon Gutwein , Daria Lazic , Thomas Walter , Sabine Taschner-Mandl , Roxane Licandro

Automatic detection and segmentation of cells and nuclei in microscopy images is important for many biological applications. Recent successful learning-based approaches include per-pixel cell segmentation with subsequent pixel grouping, or…

Computer Vision and Pattern Recognition · Computer Science 2018-11-09 Uwe Schmidt , Martin Weigert , Coleman Broaddus , Gene Myers

Accurate nuclei segmentation in microscopy whole slide images (WSIs) remains challenging due to variability in staining, imaging conditions, and tissue morphology. We propose CellGenNet, a knowledge distillation framework for robust…

Computer Vision and Pattern Recognition · Computer Science 2025-11-20 Srijan Ray , Bikesh K. Nirala , Jason T. Yustein , Sundaresh Ram

Nuclei segmentation is one of the important tasks for whole slide image analysis in digital pathology. With the drastic advance of deep learning, recent deep networks have demonstrated successful performance of the nuclei segmentation task.…

Computer Vision and Pattern Recognition · Computer Science 2019-07-23 Inwan Yoo , Donggeun Yoo , Kyunghyun Paeng

Nuclei detection and segmentation in hematoxylin and eosin-stained (H&E) tissue images are important clinical tasks and crucial for a wide range of applications. However, it is a challenging task due to nuclei variances in staining and…

Image and Video Processing · Electrical Eng. & Systems 2023-10-09 Fabian Hörst , Moritz Rempe , Lukas Heine , Constantin Seibold , Julius Keyl , Giulia Baldini , Selma Ugurel , Jens Siveke , Barbara Grünwald , Jan Egger , Jens Kleesiek

Nuclei segmentation is a fundamental prerequisite in the digital pathology workflow. The development of automated methods for nuclei segmentation enables quantitative analysis of the wide existence and large variances in nuclei morphometry…

Computer Vision and Pattern Recognition · Computer Science 2024-01-17 Yi Lin , Zeyu Wang , Dong Zhang , Kwang-Ting Cheng , Hao Chen

Quantitative analysis of cell nuclei in microscopic images is an essential yet challenging source of biological and pathological information. The major challenge is accurate detection and segmentation of densely packed nuclei in images…

Quantitative Methods · Quantitative Biology 2019-11-14 Linqing Feng , Jun Ho Song , Jiwon Kim , Soomin Jeong , Jin Sung Park , Jinhyun Kim

Semantic and instance segmentation algorithms are two general yet distinct image segmentation solutions powered by Convolution Neural Network. While semantic segmentation benefits extensively from the end-to-end training strategy, instance…

Computer Vision and Pattern Recognition · Computer Science 2021-03-30 Jianfeng Cao , Hong Yan