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Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit…

We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode…

Optics · Physics 2016-02-17 Juan Miguel Pérez , Marc Jofre , Pedro Martínez , Valerio Pruneri

We propose a portable cartridge based lab-on-a-chip platform. The main sensing mechanism uses high frequency acoustic waves. Feasibility of two different transducers has been investigated for biological measurements. We used zincoxide (ZnO)…

Medical Physics · Physics 2017-10-17 Seyedfakhreddin Nabavi

We present a wide-field multi-pass implementation of label-free imaging flow cytometry. Our technique is shown for high-speed flow imaging of ensembles of human red blood cells with up to four passes, demonstrating x4 enhancement in…

Laser-driven X-ray sources are an emerging alternative to conventional X-ray tubes and synchrotron sources. We present results on microtomographic X-ray imaging of a cancellous human bone sample using synchrotron-like betatron radiation.…

Poor access to eye care is a major global challenge that could be ameliorated by low-cost, portable, and easy-to-use diagnostic technologies. Diffuser-based imaging has the potential to enable inexpensive, compact optical systems that can…

Medical Physics · Physics 2020-06-23 Yunzhe Li , Gregory N. McKay , Nicholas J. Durr , Lei Tian

Interferometric scattering microscopy has been a very promising technology for highly sensitive label-free imaging of a broad spectrum of biological nanoparticles from proteins to viruses in a high-throughput manner. Although it can reveal…

Optics · Physics 2021-08-11 Celalettin Yurdakul , Haonan Zong , Yeran Bai , Ji-Xin Cheng , M. Selim Unlu

Bone marrow cytology is required to make a hematological diagnosis, influencing critical clinical decision points in hematology. However, bone marrow cytology is tedious, limited to experienced reference centers and associated with high…

Image and Video Processing · Electrical Eng. & Systems 2021-07-09 Rohollah Moosavi Tayebi , Youqing Mu , Taher Dehkharghanian , Catherine Ross , Monalisa Sur , Ronan Foley , Hamid R. Tizhoosh , Clinton JV Campbell

In computational imaging, hardware for signal sampling and software for object reconstruction are designed in tandem for improved capability. Examples of such systems include computed tomography (CT), magnetic resonance imaging (MRI), and…

Image and Video Processing · Electrical Eng. & Systems 2022-11-01 Andrew Olsen , Yolanda Hu , Vidya Ganapati

One of the challenges facing the adoption of digital pathology workflows for clinical use is the need for automated quality control. As the scanners sometimes determine focus inaccurately, the resultant image blur deteriorates the scanned…

Image and Video Processing · Electrical Eng. & Systems 2018-11-16 Mahdi S. Hosseini , Yueyang Zhang , Lyndon Chan , Konstantinos N. Plataniotis , Jasper A. Z. Brawley-Hayes , Savvas Damaskinos

Deep learning has achieved spectacular performance in image and speech recognition and synthesis. It outperforms other machine learning algorithms in problems where large amounts of data are available. In the area of measurement technology,…

Quantitative Methods · Quantitative Biology 2019-08-20 Yueqin Li , Ata Mahjoubfar , Claire Lifan Chen , Kayvan Reza Niazi , Li Pei , Bahram Jalali

Coherent diffractive imaging (CDI) enables lensless imaging with experimental simplicity and a flexible field of view, yet its resolution is fundamentally constrained by the Abbe diffraction limit. To overcome this limitation, we introduce…

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…

Instrumentation and Detectors · Physics 2023-12-21 Michela Esposito , Nicole Schieber , Alessandro Olivo , Yannick Schwab , Marco Endrizzi

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Advances in the development of free-electron lasers offer the realistic prospect of high-resolution imaging to study the nanoworld on the time-scale of atomic motions. We identify X-ray Fourier Transform holography, (FTH) as a promising…

While analysing rare blood cell aggregates remains challenging in automated haematology, they could markedly advance label-free functional diagnostics. Conventional flow cytometers efficiently perform cell counting with leukocyte…

High throughput real-time instruments are needed to acquire large data sets for detection and classification of rare events. Enabled by the photonic time stretch digitizer, a new class of instruments with record throughputs have led to the…

Optics · Physics 2015-03-20 Bahram Jalali , Ata Mahjoubfar , Claire L. Chen

Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases. Due to limitations…

In this work, we present and investigate the novel blind inverse problem of position-blind ptychography, i.e., ptychographic phase retrieval without any knowledge of scan positions, which then must be recovered jointly with the image. The…

Image and Video Processing · Electrical Eng. & Systems 2026-03-18 Simon Welker , Lorenz Kuger , Tim Roith , Berthy Feng , Martin Burger , Timo Gerkmann , Henry Chapman

The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Ganna Platonova , Dalibor Stys , Pavel Soucek , Kirill Lonhus , Jan Valenta , Renata Rychtarikova