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Differentiating signals from the background in micrographs is a critical initial step for cryogenic electron microscopy (cryo-EM), yet it remains laborious due to low signal-to-noise ratio (SNR), the presence of contaminants and densely…

Image and Video Processing · Electrical Eng. & Systems 2025-09-30 Szu-Chi Chung , Po-Cheng Chou

Background and Objective: Wilson statistics describe well the power spectrum of proteins at high frequencies. Therefore, it has found several applications in structural biology, e.g., it is the basis for sharpening steps used in cryogenic…

Quantitative Methods · Quantitative Biology 2022-05-03 Marc Aurèle Gilles , Amit Singer

Recently, convolutional auto-encoders (CAE) were introduced for image coding. They achieved performance improvements over the state-of-the-art JPEG2000 method. However, these performances were obtained using massive CAEs featuring a large…

Image and Video Processing · Electrical Eng. & Systems 2022-09-13 Cyprien Gille , Frédéric Guyard , Marc Antonini , Michel Barlaud

We consider the multi-target detection problem of estimating a two-dimensional target image from a large noisy measurement image that contains many randomly rotated and translated copies of the target image. Motivated by single-particle…

Image and Video Processing · Electrical Eng. & Systems 2022-09-05 Tamir Bendory , Ti-Yen Lan , Nicholas F. Marshall , Iris Rukshin , Amit Singer

Cryo-Electron Microscopy (cryo-EM) has become an extremely powerful method for resolving structural details of large biomolecular complexes. However, challenging problems in single-particle methods remain open because of (1) the low…

Biomolecules · Quantitative Biology 2019-08-12 Shengnan Lyu , Christian Wülker , Yuqing Pan , Amitesh S. Jayaraman , Jianhao Zheng , Yilin Cai , Gregory S. Chirikjian

Cryo-electron microscopy (cryo-EM) has revolutionized experimental protein structure determination. Despite advances in high resolution reconstruction, a majority of cryo-EM experiments provide either a single state of the studied…

We study a class of orbit recovery problems in which we observe independent copies of an unknown element of $\mathbb{R}^p$, each linearly acted upon by a random element of some group (such as $\mathbb{Z}/p$ or $\mathrm{SO}(3)$) and then…

Statistics Theory · Mathematics 2023-06-26 Afonso S. Bandeira , Ben Blum-Smith , Joe Kileel , Amelia Perry , Jonathan Niles-Weed , Alexander S. Wein

Background and Objective: The contrast of cryo-EM images varies from one to another, primarily due to the uneven thickness of the ice layer. This contrast variation can affect the quality of 2-D class averaging, 3-D ab-initio modeling, and…

Computer Vision and Pattern Recognition · Computer Science 2022-07-01 Yunpeng Shi , Amit Singer

Over the past decade, cryogenic electron microscopy (cryo-EM) has emerged as a primary method for determining near-native, near-atomic resolution 3D structures of biological macromolecules. In order to meet increasing demand for cryo-EM,…

Image and Video Processing · Electrical Eng. & Systems 2022-01-19 Paul T. Kim , Alex J. Noble , Anchi Cheng , Tristan Bepler

In the past decade, deep conditional generative models have revolutionized the generation of realistic images, extending their application from entertainment to scientific domains. Single-particle cryo-electron microscopy (cryo-EM) is…

Computer Vision and Pattern Recognition · Computer Science 2024-10-01 Jiakai Zhang , Qihe Chen , Yan Zeng , Wenyuan Gao , Xuming He , Zhijie Liu , Jingyi Yu

We study the multi-target detection problem of recovering a target signal from a noisy measurement that contains multiple copies of the signal at unknown locations. Motivated by the structure reconstruction problem in cryo-electron…

Signal Processing · Electrical Eng. & Systems 2022-05-17 Ye'Ela Shalit , Ran Weber , Asaf Abas , Shay Kreymer , Tamir Bendory

A common task in cryo-electron microscopy (cryo-EM) data processing is to compare three-dimensional density maps of macromolecules. In this paper, we propose an algorithm for aligning three-dimensional density maps that exploits common…

Image and Video Processing · Electrical Eng. & Systems 2023-02-27 Yael Harpaz , Yoel Shkolnisky

Cryo-electron microscopy (cryo-EM) has revolutionized structural biology by enabling near-atomic-level visualization of biomolecular assemblies. However, the exponential growth in cryo-EM data throughput and complexity, coupled with diverse…

Quantitative Methods · Quantitative Biology 2026-02-25 Weining Fu , Kai Shu , Kui Xu , Qiangfeng Cliff Zhang

Cryo-electron microscopy (cryo-EM) studies using single particle reconstruction are extensively used to reveal structural information on macromolecular complexes. Aiming at the highest achievable resolution, state of the art electron…

A general framework for solving image inverse problems is introduced in this paper. The approach is based on Gaussian mixture models, estimated via a computationally efficient MAP-EM algorithm. A dual mathematical interpretation of the…

Computer Vision and Pattern Recognition · Computer Science 2010-06-16 Guoshen Yu , Guillermo Sapiro , Stéphane Mallat

Although EDM aims to unify the design space of diffusion models, its reliance on fixed Gaussian noise prevents it from explaining emerging flow-based methods that diffuse arbitrary noise. Moreover, our study reveals that EDM's forcible…

Computer Vision and Pattern Recognition · Computer Science 2026-03-06 Xingyu Qiu , Mengying Yang , Xinghua Ma , Dong Liang , Fanding Li , Gongning Luo , Wei Wang , Kuanquan Wang , Shuo Li

Cryo-EM is a vital technique for determining 3D structure of biological molecules such as proteins and viruses. The cryo-EM reconstruction problem is challenging due to the high noise levels, the missing poses of particles, and the…

Quantitative Methods · Quantitative Biology 2024-06-05 Larissa de Ruijter , Gabriele Cesa

Single particle cryo-electron microscopy is a vital tool for 3D characterization of protein structures. A typical workflow involves acquiring projection images of a collection of randomly oriented particles, picking and classifying…

Image and Video Processing · Electrical Eng. & Systems 2021-03-22 S. V. Venkatakrishnan , Puneet Juneja , Hugh O'Neill

The application that motivates this paper is molecular imaging at the atomic level. When discretized at sub-atomic distances, the volume is inherently sparse. Noiseless measurements from an imaging technology can be modeled by convolution…

Data Analysis, Statistics and Probability · Physics 2015-05-13 Michael Ting , Raviv Raich , Alfred O. Hero

Cryo-electron microscopy (cryo-EM) is a widely used technique for recovering the 3-D structure of biological molecules from a large number of experimentally generated noisy 2-D tomographic projection images of the 3-D structure, taken from…

Numerical Analysis · Mathematics 2025-11-17 Jeremy Hoskins , Yuehaw Khoo , Oscar Mickelin , Amit Singer , Yuguan Wang
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