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We investigate the application of the common circle method for estimating sample motion in optical diffraction tomography (ODT) of sub-millimeter sized biological tissue. When samples are confined via contact-free acoustical force fields,…

Numerical Analysis · Mathematics 2026-02-20 Michael Quellmalz , Mia Kvåle Løvmo , Simon Moser , Franziska Strasser , Monika Ritsch-Marte

One of the challenges in single particle reconstruction in cryo-electron microscopy is to find a three-dimensional model of a molecule using its two-dimensional noisy projection-images. In this paper, we propose a robust "angular…

Computational Geometry · Computer Science 2020-01-08 Gabi Pragier , Yoel Shkolnisky

Tomography is the three-dimensional reconstruction of an object from images taken at different angles. The term classical tomography is used, when the imaging beam travels in straight lines through the object. This assumption is valid for…

Quantitative Methods · Quantitative Biology 2016-10-10 Paul Müller , Mirjam Schürmann , Jochen Guck

Very short pulses of x-ray free-electron lasers opened the way to obtain diffraction signal from single particles beyond the radiation dose limit. For 3D structure reconstruction many patterns are recorded in the object's unknown…

Data Analysis, Statistics and Probability · Physics 2011-05-11 Gábor Bortel , Miklós Tegze

Diffraction tomography is a widely used inverse scattering technique for quantitative imaging of weakly scattering media. In its conventional formulation, diffraction tomography assumes monochromatic plane wave illumination. This…

Numerical Analysis · Mathematics 2026-03-11 Peter Elbau , Noemi Naujoks , Otmar Scherzer

We revisit the topic of common lines between projection images in single particle cryo-electron microscopy (cryo-EM). We derive a novel low-rank constraint on a certain $2n \times n$ matrix storing properly-scaled basis vectors for the…

Optimization and Control · Mathematics 2024-06-10 Tommi Muller , Adriana L. Duncan , Eric J. Verbeke , Joe Kileel

Diffraction tomography is an inverse scattering technique used to reconstruct the spatial distribution of the material properties of a weakly scattering object. The object is exposed to radiation, typically light or ultrasound, and the…

Numerical Analysis · Mathematics 2024-03-26 Clemens Kirisits , Noemi Naujoks , Otmar Scherzer

Cryo-electron microscopy is a state-of-the-art method for determining high-resolution three-dimensional models of molecules, from their two-dimensional projection images taken by an electron microscope. A crucial step in this method is to…

Image and Video Processing · Electrical Eng. & Systems 2022-12-16 Eitan Rosen , Yoel Shkolnisky

A major challenge in single particle reconstruction from cryo-electron microscopy is to establish a reliable ab-initio three-dimensional model using two-dimensional projection images with unknown orientations. Common-lines based methods…

Machine Learning · Computer Science 2013-04-11 Lanhui Wang , Amit Singer , Zaiwen Wen

An approach to diffraction tomography is investigated for two-dimensional image reconstruction of objects surrounded by an arbitrarily-shaped curve of sources and receivers. Based on the integral theorem of Helmholtz and Kirchhoff, the…

Mathematical Physics · Physics 2014-11-20 G. T. Clement

In this paper, we study the mathematical imaging problem of diffraction tomography (DT), which is an inverse scattering technique used to find material properties of an object by illuminating it with probing waves and recording the…

Numerical Analysis · Mathematics 2023-05-16 Florian Faucher , Clemens Kirisits , Michael Quellmalz , Otmar Scherzer , Eric Setterqvist

A major challenge in single particle reconstruction methods using cryo-electron microscopy is to attain a resolution sufficient to interpret fine details in three-dimensional (3D) macromolecular structures. Obtaining high resolution 3D…

Numerical Analysis · Mathematics 2013-07-23 Lanhui Wang , Yoel Shkolnisky , Amit Singer

In this paper, we study the mathematical imaging problem of optical diffraction tomography (ODT) for the scenario of a microscopic rigid particle rotating in a trap created, for instance, by acoustic or optical forces. Under the influence…

Cryo-Electron Microscopy (Cryo-EM) is a Nobel prize-winning technology for determining the 3D structure of particles at near-atomic resolution. A fundamental step in the recovering of the 3D single-particle structure is to align its 2D…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 Koby Bibas , Gili Weiss-Dicker , Dana Cohen , Noa Cahan , Hayit Greenspan

Coherent diffraction imaging methods enable imaging beyond lens-imposed resolution limits. In these methods, the object can be recovered by minimizing an error metric that quantifies the difference between diffraction patterns as observed,…

Image and Video Processing · Electrical Eng. & Systems 2019-07-24 Saugat Kandel , S. Maddali , Marc Allain , Stephan O. Hruszkewycz , Chris Jacobsen , Youssef S G Nashed

This work addresses the problem of uniquely determining a rotational motion from continuous time-dependent measurements within the frameworks of parallel-beam and diffraction tomography. The motivation stems from the challenge of imaging…

Functional Analysis · Mathematics 2025-10-22 Peter Elbau , Denise Schmutz

We consider the imaging problem of the reconstruction of a three-dimensional object via optical diffraction tomography under the assumptions of the Born approximation. Our focus lies in the situation that a rigid object performs an…

Numerical Analysis · Mathematics 2024-07-11 Robert Beinert , Michael Quellmalz

Decomposition of tomographic reconstructions has many different practical application. We propose two new reconstruction methods that combines the task of tomographic reconstruction with object decomposition. We demonstrate these…

Computational Engineering, Finance, and Science · Computer Science 2017-08-25 Rasmus Dalgas Kongskov , Yiqiu Dong

In many computed tomography (CT) imaging applications, it is important to rapidly collect data from an object that is moving or changing with time. Tomographic acquisition is generally assumed to be step-and-shoot, where the object is…

Image and Video Processing · Electrical Eng. & Systems 2022-08-02 Soumendu Majee , Selin Aslan , Doga Gursoy , Charles A. Bouman

This paper concerns diffraction-tomographic reconstruction of an object characterized by its scattering potential. We establish a rigorous generalization of the Fourier diffraction theorem in arbitrary dimension, giving a precise relation…

Numerical Analysis · Mathematics 2026-03-30 Clemens Kirisits , Michael Quellmalz , Eric Setterqvist
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