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Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…

Biological Physics · Physics 2018-06-12 Mathias Lechelon , Marco Pettini

This study consolidates Multi-Level Starlet Segmentation (MLSS) and Multi-Level Starlet Optimal Segmentation (MLSOS), techniques for photomicrograph segmentation that use starlet wavelet detail levels to separate areas of interest in an…

Computer Vision and Pattern Recognition · Computer Science 2017-06-14 Alexandre Fioravante de Siqueira , Flávio Camargo Cabrera , Aylton Pagamisse , Aldo Eloizo Job

We have used a gold nanohole array to trap single polystyrene nanoparticles, with a mean diameter of 30 nm, into separated hot spots located at connecting nanoslot regions. A high trap stiffness of approximately 0.85 fN/(nmmW) at a low…

Optics · Physics 2019-08-16 Xue Han , Viet Giang Truong , Sile Nic Chormaic

UV-Vis absorption spectroscopy is frequently used to characterize the size and shape of gold nanoparticles. We present a full-spectrum model that yields reliable results for the commonly encountered case of mixtures of spheres and rods in…

Soft Condensed Matter · Physics 2015-04-07 Kostyantyn Slyusarenko , Benjamin Abécassis , Patrick Davidson , Doru Constantin

We report an all-electric integrable electron focusing lens in n-type GaAs. It is shown that a pronounced focusing peak takes place when the focal point aligns with an on-chip detector. The intensity and full width half maximum (FWHM) of…

Mesoscale and Nanoscale Physics · Physics 2020-02-14 Chengyu Yan , Michael Pepper , Patrick See , Ian Farrer , David Ritchie , Jonathan Griffiths

Observing the individual building blocks of matter is one of the primary goals of microscopy. The invention of the scanning tunneling microscope [1] revolutionized experimental surface science in that atomic-scale features on a solid-state…

Materials Science · Physics 2008-08-04 Jannik C. Meyer , C. O. Girit , M. F. Crommie , A. Zettl

Understanding the interplay between the structure, composition and opto-electronic properties of semiconductor nano-objects requires combining transmission electron microscopy (TEM) based techniques with electrical and optical measurements…

Despite advances in low-light level detection, single-photon methods such as photon correlation have rarely been used in the context of imaging. The few demonstrations, for example of sub-diffraction limited imaging utilizing quantum…

Optics · Physics 2017-03-14 Yonatan Israel , Ron Tenne , Dan Oron , Yaron Silberberg

Electron microscopy is a powerful tool for visualizing the shapes of sub-nanometer objects. However, contrast is not in proportional to density distribution, and therefore achieving a quantitative understanding of specimens is not…

Instrumentation and Detectors · Physics 2020-05-14 Masato Tomita , Yukinori Nagatani , Kazuyoshi Murata , Atsushi Momose

The photoluminescence (PL) quantum yield of single-walled carbon nanotubes (SWNTs) is relatively low, with various quenching effects by metallic species reported in the literature. Here, we report the first case of metal enhanced…

Materials Science · Physics 2010-10-29 Guosong Hong , Scott M. Tabakman , Kevin Welsher , Hailiang Wang , Xinran Wang , Hongjie Dai

In electron microscopy, charging of non-conductive biological samples by focused electron beams hinders their high-resolution imaging. Gold or platinum coatings have been commonly used to prevent such sample charging, but it disables…

The orientations of the red galaxies in a filament are aligned with the orientation of the filament. We thus develop a location-alignment-method (LAM) of detecting filaments around clusters of galaxies, which uses both the alignments of red…

Astrophysics of Galaxies · Physics 2015-10-29 Yu Rong , Yuan Liu , Shuang-Nan Zhang

In this study, we present a computational framework tailored for the precise detection and comprehensive analysis of nanoparticles within scanning electron microscopy (SEM) images. The primary objective of this framework revolves around the…

Image and Video Processing · Electrical Eng. & Systems 2023-08-21 Aidan S. Wright , Nathaniel P. Youmans , Enrique F. Valderrama Araya

Purpose: To develop and apply multiscale Monte Carlo (MC) simulations to assess variations in nucleus and cytoplasm dose enhancement factors (n,cDEFs) over tumor-scale volumes. Methods: The intrinsic variation of n,cDEFs (due to variations…

Medical Physics · Physics 2023-05-04 Martin P. Martinov , Elizabeth M. Fletcher , Rowan M. Thomson

In this study, a radiomics approach was extended to optical fluorescence molecular imaging data for tissue classification, termed 'optomics'. Fluorescence molecular imaging is emerging for precise surgical guidance during head and neck…

Ultrasensitive optical detection of nanometer-scaled particles is highly desirable for applications in early-stage diagnosis of human diseases, environmental monitoring, and homeland security, but remains extremely difficult due to ultralow…

DNA and protein microarrays are a high-throughput technology that allow the simultaneous quantification of tens of thousands of different biomolecular species. The mediocre sensitivity and dynamic range of traditional fluorescence…

Biomolecules · Quantitative Biology 2018-01-24 Derin Sevenler , George Daaboul , Fulya Ekiz-Kanik , M. Selim Unlu

Raman Spectroscopy offers an in-situ, rapid, and non-destructive characterization tool for chemical analysis of diverse samples with no or minimal preparation. However, due to the inherent weak signal of conventional Raman spectroscopy,…

Electron beam imaging is a common technique used for characterizing the morphology of plasmonic nanostructures. During the imaging process, the electron beam interacts with traces of organic material in the chamber and produces a well-know…

Mesoscale and Nanoscale Physics · Physics 2013-11-13 Mingxia Song , Gérard Colas-des-Francs , Alexandre Bouhelier

We demonstrate a hard x-ray probe of laser-aligned small molecules. To align small molecules with optical lasers, high intensities at nonresonant wavelengths are necessary. We use 95 ps pulses focused to 40 mum from an 800 nm Ti:sapphire…