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Cryo-electron microscopy is a technique in structural biology for discovering/determining the 3D structure of small molecules. A key step in this process is detecting common lines of intersection between unknown embedded image planes. We…

Algebraic Geometry · Mathematics 2014-02-25 David Dynerman

The single-particle cryo-EM field faces the persistent challenge of preferred orientation, lacking general computational solutions. We introduce cryoPROS, an AI-based approach designed to address the above issue. By generating the auxiliary…

Biomolecules · Quantitative Biology 2023-09-27 Hui Zhang , Dihan Zheng , Qiurong Wu , Nieng Yan , Zuoqiang Shi , Mingxu Hu , Chenglong Bao

Imaging of liquids and cryogenic biological materials by electron microscopy has been recently enabled by innovative approaches for specimen preparation and the fast development of optimised instruments for cryo-enabled electron microscopy…

Cryo-electron tomography (cryoET) is a technique that captures images of biological samples at different tilts, preserving their native state as much as possible. Along with the partial tilt series and noise, one of the major challenges in…

Signal Processing · Electrical Eng. & Systems 2023-07-26 Vinith Kishore , Valentin Debarnot , Ivan Dokmanić

Online reconstruction is key for monitoring purposes and real time analysis in High Energy and Nuclear Physics experiments. A necessary component of reconstruction algorithms is particle identification that combines information left by a…

Instrumentation and Detectors · Physics 2026-01-13 Richard Tyson , Gagik Gavalian

Knowledge of a protein's atomic conformational ensemble is critical to determining its function, yet state-of-the-art ensemble prediction models are limited by lack of high-quality conformational data from simulation or experiment. Recent…

Machine Learning · Computer Science 2026-05-12 Jay Shenoy , Miro Astore , Axel Levy , Frédéric Poitevin , Sonya M. Hanson , Gordon Wetzstein

Principal component analysis (PCA) is arguably the most widely used dimension-reduction method for vector-type data. When applied to a sample of images, PCA requires vectorization of the image data, which in turn entails solving an…

Image and Video Processing · Electrical Eng. & Systems 2021-03-02 Szu-Chi Chung , Shao-Hsuan Wang , Po-Yao Niu , Su-Yun Huang , Wei-Hau Chang , I-Ping Tu

Structural dynamics of macromolecules is critical to their structural-function relationship. Cryogenic electron microscopy (CryoEM) provides snapshots of vitrified protein at different compositional and conformational states, and the…

Quantitative Methods · Quantitative Biology 2026-01-27 Muyuan Chen , Muchen Li , Renjie Liao

Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding of ribosomal biology. Here, we provide a historical perspective…

Biomolecules · Quantitative Biology 2023-08-31 Samantha M. Webster , Mira B. May , Barrett M. Powell , Joseph H. Davis

While electron microscopy offers crucial atomic-resolution insights into structure-property relationships, radiation damage severely limits its use on beam-sensitive materials like proteins and 2D materials. To overcome this challenge, we…

Computer Vision and Pattern Recognition · Computer Science 2025-07-18 Zifei Wang , Zian Mao , Xiaoya He , Xi Huang , Haoran Zhang , Chun Cheng , Shufen Chu , Tingzheng Hou , Xiaoqin Zeng , Yujun Xie

We present an end-to-end reconstruction algorithm to build particle candidates from detector hits in next-generation granular calorimeters similar to that foreseen for the high-luminosity upgrade of the CMS detector. The algorithm exploits…

Instrumentation and Detectors · Physics 2022-10-03 Shah Rukh Qasim , Nadezda Chernyavskaya , Jan Kieseler , Kenneth Long , Oleksandr Viazlo , Maurizio Pierini , Raheel Nawaz

Accurate computational ptychographic phase reconstructions are enabled by fast direct-electron cameras with high dynamic ranges used for four-dimensional scanning transmission electron microscopy (4D-STEM). The availability of open software…

Materials Science · Physics 2025-05-01 Toma Susi

A major limitation of two-dimensional scanning electron microscopy (SEM) in imaging porous membranes is its inability to resolve three-dimensional pore architecture and interconnectivity, which are critical factors governing membrane…

Much of the exotic functionality of prime interest in quantum materials emerges from structural and electronic ground states that can only be accessed at cryogenic temperatures. Understanding device operation therefore requires structural…

We study a class of orbit recovery problems in which we observe independent copies of an unknown element of $\mathbb{R}^p$, each linearly acted upon by a random element of some group (such as $\mathbb{Z}/p$ or $\mathrm{SO}(3)$) and then…

Statistics Theory · Mathematics 2023-06-26 Afonso S. Bandeira , Ben Blum-Smith , Joe Kileel , Amelia Perry , Jonathan Niles-Weed , Alexander S. Wein

Modern technology for producing extremely bright and coherent X-ray laser pulses provides the possibility to acquire a large number of diffraction patterns from individual biological nanoparticles, including proteins, viruses, and DNA.…

Methodology · Statistics 2018-07-11 Stefan Engblom , Carl Nettelblad , Jing Liu

Transmission electron microscopes use electrons with wavelengths of a few picometers, potentially capable of imaging individual atoms in solids at a resolution ultimately set by the intrinsic size of an atom. Unfortunately, due to…

In this paper, we demonstrate a computationally efficient new approach based on deep learning (DL) techniques for analysis, design, and optimization of electromagnetic (EM) nanostructures. We use the strong correlation among features of a…

Machine Learning · Computer Science 2020-02-13 Yashar Kiarashinejad , Sajjad Abdollahramezani , Ali Adibi

Understanding protein flexibility and its dynamic interactions with other molecules is essential for studying protein function. Although cryogenic electron microscopy(cryo-EM) provides an opportunity to observe macromolecular dynamics…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Bintao He , Yiran Cheng , Hongjia Li , Xiang Gao , Xin Gao , Fa Zhang , Renmin Han

It has been a general trend to develop low-voltage electron microscopes due to their high imaging contrast of the sample and low radiation damage. Atom-resolved transmission electron microscopes with voltages as low as 15-40 kV have been…

Instrumentation and Detectors · Physics 2015-12-29 Wei-Tse Chang , Chun-Yueh Lin , Wei-Hao Hsu , Mu-Tung Chang , Yi-Sheng Chen , En-Te Hwu , Ing-Shouh Hwang
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