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The nematode Caenorhabditis elegans (C. elegans) is used as a model organism to better understand developmental biology and neurobiology. C. elegans features an invariant cell lineage, which has been catalogued and observed using…

Computer Vision and Pattern Recognition · Computer Science 2022-08-03 Andrew Lauziere , Ryan Christensen , Hari Shroff

We propose a novel weakly supervised method to improve the boundary of the 3D segmented nuclei utilizing an over-segmented image. This is motivated by the observation that current state-of-the-art deep learning methods do not result in…

Computer Vision and Pattern Recognition · Computer Science 2021-08-31 S. Shailja , Jiaxiang Jiang , B. S. Manjunath

3D cell tracking in a living organism has a crucial role in live cell image analysis. Cell tracking in C. elegans has two difficulties. First, cell migration in a consecutive frame is large since they move their head during scanning.…

Computer Vision and Pattern Recognition · Computer Science 2024-03-21 Kaito Shiku , Hiromitsu Shirai , Takeshi Ishihara , Ryoma Bise

We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha

Determining cell identities in imaging sequences is an important yet challenging task. The conventional method for cell identification is via cell tracking, which is complex and can be time-consuming. In this study, we propose an innovative…

Quantitative Methods · Quantitative Biology 2024-03-05 Baiyang Dai , Jiamin Yang , Hari Shroff , Patrick La Riviere

Tracking many cells in time-lapse 3D image sequences is an important challenging task of bioimage informatics. Motivated by a study of brain-wide 4D imaging of neural activity in C. elegans, we present a new method of multi-cell tracking.…

Computer Vision and Pattern Recognition · Computer Science 2018-01-18 Osamu Hirose , Shotaro Kawaguchi , Terumasa Tokunaga , Yu Toyoshima , Takayuki Teramoto , Sayuri Kuge , Takeshi Ishihara , Yuichi Iino , Ryo Yoshida

Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…

Computer Vision and Pattern Recognition · Computer Science 2017-05-10 Saad Ullah Akram , Juho Kannala , Lauri Eklund , Janne Heikkilä

Recently, deep learning-based methods achieved promising performance in nuclei detection and classification applications. However, training deep learning-based methods requires a large amount of pixel-wise annotated data, which is…

Image and Video Processing · Electrical Eng. & Systems 2024-01-17 Nazanin Moradinasab , Rebecca A. Deaton , Laura S. Shankman , Gary K. Owens , Donald E. Brown

Nuclei instance segmentation on histopathology images is of great clinical value for disease analysis. Generally, fully-supervised algorithms for this task require pixel-wise manual annotations, which is especially time-consuming and…

Computer Vision and Pattern Recognition · Computer Science 2023-06-06 Yang Zhou , Yongjian Wu , Zihua Wang , Bingzheng Wei , Maode Lai , Jianzhong Shou , Yubo Fan , Yan Xu

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

Quantitative analysis of cell nuclei in microscopic images is an essential yet challenging source of biological and pathological information. The major challenge is accurate detection and segmentation of densely packed nuclei in images…

Quantitative Methods · Quantitative Biology 2019-11-14 Linqing Feng , Jun Ho Song , Jiwon Kim , Soomin Jeong , Jin Sung Park , Jinhyun Kim

Microscopy structure segmentation, such as detecting cells or nuclei, generally requires a human to draw a ground truth contour around each instance. Weakly supervised approaches (e.g. consisting of only single point labels) have the…

Computer Vision and Pattern Recognition · Computer Science 2024-11-25 James Willoughby , Irina Voiculescu

Nuclei segmentation is a fundamental task in histopathology image analysis. Typically, such segmentation tasks require significant effort to manually generate accurate pixel-wise annotations for fully supervised training. To alleviate such…

Computer Vision and Pattern Recognition · Computer Science 2020-07-13 Hui Qu , Pengxiang Wu , Qiaoying Huang , Jingru Yi , Zhennan Yan , Kang Li , Gregory M. Riedlinger , Subhajyoti De , Shaoting Zhang , Dimitris N. Metaxas

The field of computational pathology has witnessed great advancements since deep neural networks have been widely applied. These networks usually require large numbers of annotated data to train vast parameters. However, it takes…

Computer Vision and Pattern Recognition · Computer Science 2022-08-11 Yixiao Zhang , Adam Kortylewski , Qing Liu , Seyoun Park , Benjamin Green , Elizabeth Engle , Guillermo Almodovar , Ryan Walk , Sigfredo Soto-Diaz , Janis Taube , Alex Szalay , Alan Yuille

The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object tracking methods. In recent years, several existing and new applications have…

Image and Video Processing · Electrical Eng. & Systems 2025-02-03 Gergely Szabó , Paolo Bonaiuti , Andrea Ciliberto , András Horváth

The tracking method based on the extreme learning machine (ELM) is efficient and effective. ELM randomly generates input weights and biases in the hidden layer, and then calculates and computes the output weights by reducing the iterative…

Machine Learning · Computer Science 2018-07-27 Jing Zhang , Huibing Wang , Yonggong Ren

Cell detection and segmentation is fundamental for all downstream analysis of digital pathology images. However, obtaining the pixel-level ground truth for single cell segmentation is extremely labor intensive. To overcome this challenge,…

Computer Vision and Pattern Recognition · Computer Science 2019-10-29 Alireza Chamanzar , Yao Nie

Cell segmentation and tracking in microscopy images are of great significance to new discoveries in biology and medicine. In this study, we propose a novel approach to combine cell segmentation and cell tracking into a unified end-to-end…

Computer Vision and Pattern Recognition · Computer Science 2021-02-23 Yuqian Chen , Yang Song , Chaoyi Zhang , Fan Zhang , Lauren O'Donnell , Wojciech Chrzanowski , Weidong Cai

Finding an optimal correspondence between point sets is a common task in computer vision. Existing techniques assume relatively simple relationships among points and do not guarantee an optimal match. We introduce an algorithm capable of…

Computer Vision and Pattern Recognition · Computer Science 2023-01-11 Andrew Lauziere , Ryan Christensen , Hari Shroff , Radu Balan

Detection, segmentation and tracking of fruits and vegetables are three fundamental tasks for precision agriculture, enabling robotic harvesting and yield estimation applications. However, modern algorithms are data hungry and it is not…

Computer Vision and Pattern Recognition · Computer Science 2023-01-30 Thomas A. Ciarfuglia , Ionut M. Motoi , Leonardo Saraceni , Mulham Fawakherji , Alberto Sanfeliu , Daniele Nardi
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