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Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object tracking methods. In recent years, several existing and new applications have…

Image and Video Processing · Electrical Eng. & Systems 2025-02-03 Gergely Szabó , Paolo Bonaiuti , Andrea Ciliberto , András Horváth

Studying cell morphology changes in time is critical to understanding cell migration mechanisms. In this work, we present a deep learning-based workflow to segment cancer cells embedded in 3D collagen matrices and imaged with phase-contrast…

Computer Vision and Pattern Recognition · Computer Science 2021-12-17 Estibaliz Gómez-de-Mariscal , Hasini Jayatilaka , Özgün Çiçek , Thomas Brox , Denis Wirtz , Arrate Muñoz-Barrutia

Automatic cell tracking in dense environments is plagued by inaccurate correspondences and misidentification of parent-offspring relationships. In this paper, we introduce a novel cell tracking algorithm named DenseTrack, which integrates…

Image and Video Processing · Electrical Eng. & Systems 2024-07-08 Tanjin Taher Toma , Yibo Wang , Andreas Gahlmann , Scott T. Acton

In this work, we describe a method for large-scale 3D cell-tracking through a segmentation selection approach. The proposed method is effective at tracking cells across large microscopy datasets on two fronts: (i) It can solve problems…

Computer Vision and Pattern Recognition · Computer Science 2024-04-15 Jordão Bragantini , Merlin Lange , Loïc Royer

Cell detection is the task of detecting the approximate positions of cell centroids from microscopy images. Recently, convolutional neural network-based approaches have achieved promising performance. However, these methods require a…

Computer Vision and Pattern Recognition · Computer Science 2021-07-20 Kazuya Nishimura , Hyeonwoo Cho , Ryoma Bise

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

Modeling the 3D structures of cells and tissues is crucial in biology. Sequential cross-sectional images from electron microscopy provide high-resolution intracellular structure information. The segmentation of complex cell structures…

Quantitative Methods · Quantitative Biology 2025-02-14 Jin Kousaka , Atsuko H. Iwane , Yuichi Togashi

The automated analysis of microscopy images is a challenge in the context of single-cell tracking and quantification. This work has as goals the study of the performance of deep learning for segmenting microscopy images and the improvement…

Quantitative Methods · Quantitative Biology 2022-10-05 André O. Françani

Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…

Computer Vision and Pattern Recognition · Computer Science 2017-05-10 Saad Ullah Akram , Juho Kannala , Lauri Eklund , Janne Heikkilä

Cell nuclei segmentation is one of the most important tasks in the analysis of biomedical images. With ever-growing sizes and amounts of three-dimensional images to be processed, there is a need for better and faster segmentation methods.…

Computer Vision and Pattern Recognition · Computer Science 2017-02-20 Julian Arz , Peter Sanders , Johannes Stegmaier , Ralf Mikut

Cell detection and tracking are paramount for bio-analysis. Recent approaches rely on the tracking-by-model evolution paradigm, which usually consists of training end-to-end deep learning models to detect and track the cells on the frames…

Computer Vision and Pattern Recognition · Computer Science 2023-10-12 Lucas N. Kirsten , Cláudio R. Jung

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai

We present a weakly supervised deep learning method to perform instance segmentation of cells present in microscopy images. Annotation of biomedical images in the lab can be scarce, incomplete, and inaccurate. This is of concern when…

Computer Vision and Pattern Recognition · Computer Science 2019-08-28 Fidel A. Guerrero-Peña , Pedro D. Marrero Fernandez , Tsang Ing Ren , Alexandre Cunha

A systematic analysis of the cell behavior requires automated approaches for cell segmentation and tracking. While deep learning has been successfully applied for the task of cell segmentation, there are few approaches for simultaneous cell…

Computer Vision and Pattern Recognition · Computer Science 2024-10-28 Katharina Löffler , Ralf Mikut

We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

Cell counting is a ubiquitous, yet tedious task that would greatly benefit from automation. From basic biological questions to clinical trials, cell counts provide key quantitative feedback that drive research. Unfortunately, cell counting…

Computer Vision and Pattern Recognition · Computer Science 2018-03-02 Carlos X. Hernández , Mohammad M. Sultan , Vijay S. Pande

3D microscopy is key in the investigation of diverse biological systems, and the ever increasing availability of large datasets demands automatic cell identification methods that not only are accurate, but also can imply the uncertainty in…

Computer Vision and Pattern Recognition · Computer Science 2021-02-24 Alvaro Gomariz , Tiziano Portenier , César Nombela-Arrieta , Orcun Goksel

Determining the trajectories of cells and their lineages or ancestries in live-cell experiments are fundamental to the understanding of how cells behave and divide. This paper proposes novel online algorithms for jointly tracking and…

Signal Processing · Electrical Eng. & Systems 2021-10-29 Tran Thien Dat Nguyen , Ba-Ngu Vo , Ba-Tuong Vo , Du Yong Kim , Yu Suk Choi
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