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Analyzing the dynamic changes of cellular morphology is important for understanding the various functions and characteristics of live cells, including stem cells and metastatic cancer cells. To this end, we need to track all points on the…

Computer Vision and Pattern Recognition · Computer Science 2023-03-16 Junbong Jang , Kwonmoo Lee , Tae-Kyun Kim

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

We present an automated method to track and identify neurons in C. elegans, called "fast Deep Learning Correspondence" or fDLC, based on the transformer network architecture. The model is trained once on empirically derived synthetic data…

Quantitative Methods · Quantitative Biology 2021-07-16 Xinwei Yu , Matthew S. Creamer , Francesco Randi , Anuj K. Sharma , Scott W. Linderman , Andrew M. Leifer

Mechanical properties of the cell are important biomarkers for probing its architectural changes caused by cellular processes and/or pathologies. The development of microfluidic technologies have enabled measuring cell mechanics at…

Soft Condensed Matter · Physics 2021-05-19 Moslem Moradi , Ehssan Nazockdast

Cell segmentation and tracking in microscopy images are of great significance to new discoveries in biology and medicine. In this study, we propose a novel approach to combine cell segmentation and cell tracking into a unified end-to-end…

Computer Vision and Pattern Recognition · Computer Science 2021-02-23 Yuqian Chen , Yang Song , Chaoyi Zhang , Fan Zhang , Lauren O'Donnell , Wojciech Chrzanowski , Weidong Cai

Cell motility in higher organisms (eukaryotes) is crucial to biological functions ranging from wound healing to immune response, and also implicated in diseases such as cancer. For cells crawling on hard surfaces, significant insights into…

Soft Condensed Matter · Physics 2015-06-24 E. Tjhung , A. Tiribocchi , D. Marenduzzo , M. E. Cates

To establish the relationship between locomotory behavior and dynamics of neural circuits in the nematode C. elegans we combined molecular and theoretical approaches. In particular, we quantitatively analyzed the motion of C. elegans with…

Neurons and Cognition · Quantitative Biology 2008-06-10 Jan Karbowski , Gary Schindelman , Chris J. Cronin , Adeline Seah , Paul W. Sternberg

Accurate segmentation of 3-D cell nuclei in microscopy images is essential for the study of nuclear organization, gene expression, and cell morphodynamics. Current image segmentation methods are challenged by the complexity and variability…

Image and Video Processing · Electrical Eng. & Systems 2022-03-18 Sundaresh Ram , Jeffrey J. Rodriguez

T cells use sophisticated shape dynamics (morphodynamics) to migrate towards and neutralise infected and cancerous cells. However, there is limited quantitative understanding of the migration process in 3D extracellular matrices (ECMs) and…

Biological Physics · Physics 2022-04-11 Henry Cavanagh , Daryan Kempe , Jessica K. Mazalo , Maté Biro , Robert G. Endres

Brain research has been driven by enquiry for principles of brain structure organization and its control mechanisms. The neuronal wiring map of C. elegans, the only complete connectome available till date, presents an incredible opportunity…

Neurons and Cognition · Quantitative Biology 2016-11-28 Rahul Badhwar , Ganesh Bagler

This paper introduces a novel framework for the automated tracking of cells, with a particular focus on the challenging situation of phase contrast microscopic videos. Our framework is based on a topology preserving variational segmentation…

Quantitative Methods · Quantitative Biology 2012-03-28 Michael Moeller , Martin Burger , Peter Dieterich , Albrecht Schwab

The physical and bio-mechanical principles that govern undulatory movement on wet surfaces have important applications in physiology, physics, and engineering. The nematode {\it C. elegans}, with its highly stereotypical and functionally…

Fluid Dynamics · Physics 2015-06-03 Xiao N. Shen , J. Sznitman , P. Krajacic , T. Lamitina , P. E. Arratia

The small (1 mm) nematode $\textit{Caenorhabditis elegans}$ (Corsi [1], wormbook.org) has become widely used as a model organism; in particular, the $\textit{C. elegans}$ connectome has been completely mapped, and $\textit{C. elegans}$…

Biological Physics · Physics 2021-10-22 Anshul Singhvi , Harold M. Hastings , Jenny Magnes , Susannah G. Zhang

Current methods in multiple object tracking (MOT) rely on independent object trajectories undergoing predictable motion to effectively track large numbers of objects. Adversarial conditions such as volatile object motion and imperfect…

Image and Video Processing · Electrical Eng. & Systems 2022-07-12 Andrew Lauziere , Evan Ardiel , Stephen Xu , Hari Shroff

The swimming behaviour of microorganisms can be strongly influenced by the rheology of their fluid environment. In this manuscript, we experimentally investigate the effects of shear-thinning viscosity on the swimming behaviour of an…

Fluid Dynamics · Physics 2015-06-22 David A. Gagnon , Nathan C. Keim , Paulo E. Arratia

C. elegans locomotion is composed of switches between forward and reversal states punctuated by turns. This locomotory capability is necessary for the nematode to move towards attractive stimuli, escape noxious chemicals, and explore its…

Neurons and Cognition · Quantitative Biology 2025-01-03 Megan Morrison , Lai-Sang Young

The initiation of directional cell motion requires symmetry breaking that can happen both with or without external stimuli. During cell crawling, forces generated by the cytoskeleton and their transmission through mechanosensitive adhesions…

Cell Behavior · Quantitative Biology 2023-06-02 Yuzhu Chen , David Saintillan , Padmini Rangamani

Cell tracking is an essential tool in live-cell imaging to determine single-cell features, such as division patterns or elongation rates. Unlike in common multiple object tracking, in microbial live-cell experiments cells are growing,…

Computer Vision and Pattern Recognition · Computer Science 2023-03-20 Karina Ruzaeva , Jan-Christopher Cohrs , Keitaro Kasahara , Dietrich Kohlheyer , Katharina Nöh , Benjamin Berkels

A quantitative understanding of how sensory signals are transformed into motor outputs places useful constraints on brain function and helps reveal the brain's underlying computations. We investigate how the nematode C. elegans responds to…

Neurons and Cognition · Quantitative Biology 2020-08-18 Mochi Liu , Anuj K Sharma , Joshua W Shaevitz , Andrew M Leifer

U-Net and its variants have been demonstrated to work sufficiently well in biological cell tracking and segmentation. However, these methods still suffer in the presence of complex processes such as collision of cells, mitosis and…

Image and Video Processing · Electrical Eng. & Systems 2019-10-22 Deepak K. Gupta , Nathan de Bruijn , Andreas Panteli , Efstratios Gavves