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We present a novel approach to implement compressive sensing in laser scanning microscopes (LSM), specifically in image scanning microscopy (ISM), using a single-photon avalanche diode (SPAD) array detector. Our method addresses two…

Image and Video Processing · Electrical Eng. & Systems 2023-07-20 Ajay Gunalan , Marco Castello , Simonluca Piazza , Shunlei Li , Alberto Diaspro , Leonardo S. Mattos , Paolo Bianchini

We aim to detect all instances of a category in an image and, for each instance, mark the pixels that belong to it. We call this task Simultaneous Detection and Segmentation (SDS). Unlike classical bounding box detection, SDS requires a…

Computer Vision and Pattern Recognition · Computer Science 2014-07-08 Bharath Hariharan , Pablo Arbeláez , Ross Girshick , Jitendra Malik

In single-molecule microscopy it is necessary to locate with high precision point sources from noisy observations of the spectrum of the signal at frequencies capped by $f_c$, which is just about the frequency of natural light. This paper…

Information Theory · Computer Science 2015-04-06 Veniamin I. Morgenshtern , Emmanuel J. Candes

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

Sickle cell disease (SCD) is a major public health priority throughout much of the world, affecting millions of people. In many regions, particularly those in resource-limited settings, SCD is not consistently diagnosed. In Africa, where…

We propose a novel stimulated emission depletion (STED) microscopy based on array detection and photon reassignment. By replacing the single-point detector in traditional STED with a detector array and utilizing the photon reassignment…

Segmentation of objects in microscopy images is required for many biomedical applications. We introduce object-centric embeddings (OCEs), which embed image patches such that the spatial offsets between patches cropped from the same object…

Machine Learning · Computer Science 2023-10-13 Steffen Wolf , Manan Lalit , Henry Westmacott , Katie McDole , Jan Funke

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

Optics · Physics 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

We investigate fully self-consistent multiscale quantum-classical algorithms on current generation superconducting quantum computers, in a unified approach to tackle the correlated electronic structure of large systems in both quantum…

Super-resolution microscopes (such as STED) illuminate samples with a tiny spot, and achieve very high resolution. But structures smaller than the spot cannot be resolved in this way. Therefore, we propose a technique to solve this problem.…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

We present a new certification method for image and point cloud segmentation based on randomized smoothing. The method leverages a novel scalable algorithm for prediction and certification that correctly accounts for multiple testing,…

Machine Learning · Computer Science 2022-07-28 Marc Fischer , Maximilian Baader , Martin Vechev

Computer vision and machine learning tools offer an exciting new way for automatically analyzing and categorizing information from complex computer simulations. Here we design an ensemble machine learning framework that can independently…

Computer Vision and Pattern Recognition · Computer Science 2021-09-06 Maarja Bussov , Joonas Nättilä

A novel algorithm is proposed for segmenting an image into multiple levels using its mean and variance. Starting from the extreme pixel values at both ends of the histogram plot, the algorithm is applied recursively on sub-ranges computed…

Computer Vision and Pattern Recognition · Computer Science 2007-05-23 Siddharth Arora , Jayadev Acharya , Amit Verma , Prasanta K. Panigrahi

Understanding the structure of supramolecular complexes provides insight into their functional capabilities and how they can be modulated in the context of disease. Super-resolution microscopy (SRM) excels in performing this task by…

Quantitative Methods · Quantitative Biology 2023-08-09 Afonso Mendes , Hannah S. Heil , Simao Coelho , Christophe Leterrier , Ricardo Henriques

Computational microscopy, in which hardware and algorithms of an imaging system are jointly designed, shows promise for making imaging systems that cost less, perform more robustly, and collect new types of information. Often, the…

Computer Vision and Pattern Recognition · Computer Science 2024-02-12 Henry Pinkard , Cherry Liu , Fanice Nyatigo , Daniel A. Fletcher , Laura Waller

Automatic segmentation of microscopy images is an important task in medical image processing and analysis. Nucleus detection is an important example of this task. Mask-RCNN is a recently proposed state-of-the-art algorithm for object…

Computer Vision and Pattern Recognition · Computer Science 2021-04-01 Jeremiah W. Johnson

This paper focuses on scalability and robustness of spectral clustering for extremely large-scale datasets with limited resources. Two novel algorithms are proposed, namely, ultra-scalable spectral clustering (U-SPEC) and ultra-scalable…

Machine Learning · Computer Science 2019-03-06 Dong Huang , Chang-Dong Wang , Jian-Sheng Wu , Jian-Huang Lai , Chee-Keong Kwoh

Targeted color-dots with varying shapes and sizes in images are first exhaustively identified, and then their multiscale 2D geometric patterns are extracted for testing spatial uniformness in a progressive fashion. Based on color theory in…

Computer Vision and Pattern Recognition · Computer Science 2021-06-09 Shuting Liao , Li-Yu Liu , Ting-An Chen , Kuang-Yu Chen , Fushing Hsieh

This paper addresses the task of nuclei segmentation in high-resolution histopathological images. We propose an auto- matic end-to-end deep neural network algorithm for segmenta- tion of individual nuclei. A nucleus-boundary model is…

Computer Vision and Pattern Recognition · Computer Science 2020-03-18 Yuxin Cui , Guiying Zhang , Zhonghao Liu , Zheng Xiong , Jianjun Hu
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