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We propose a cell segmentation method for analyzing images of densely clustered cells. The method combines the strengths of marker-controlled watershed transformation and a convolutional neural network (CNN). We demonstrate the method…

Image and Video Processing · Electrical Eng. & Systems 2020-04-06 Filip Lux , Petr Matula

Single-Cell RNA sequencing (scRNA-seq) measurements have facilitated genome-scale transcriptomic profiling of individual cells, with the hope of deconvolving cellular dynamic changes in corresponding cell sub-populations to better…

Genomics · Quantitative Biology 2021-04-06 Seyednami Niyakan , Ehsan Hajiramezanali , Shahin Boluki , Siamak Zamani Dadaneh , Xiaoning Qian

Despite their superior performance, deep-learning methods often suffer from the disadvantage of needing large-scale well-annotated training data. In response, recent literature has seen a proliferation of efforts aimed at reducing the…

Computer Vision and Pattern Recognition · Computer Science 2023-07-12 Ji Yu

Characterizing patient somatic mutations through next-generation sequencing technologies opens up possibilities for refining cancer subtypes. However, catalogues of mutations reveal that only a small fraction of the genes are altered…

Computational Engineering, Finance, and Science · Computer Science 2017-01-05 Ali Burak Ünal , Öznur Taştan

Recent advancements in single-cell genomics necessitate precision in gene panel selection to interpret complex biological data effectively. Those methods aim to streamline the analysis of scRNA-seq data by focusing on the most informative…

Artificial Intelligence · Computer Science 2024-06-12 Weiliang Zhang , Zhen Meng , Dongjie Wang , Min Wu , Kunpeng Liu , Yuanchun Zhou , Meng Xiao

The locations of different mRNA molecules can be revealed by multiplexed in situ RNA detection. By assigning detected mRNA molecules to individual cells, it is possible to identify many different cell types in parallel. This in turn enables…

Information Theory · Computer Science 2023-12-08 Axel Andersson , Andrea Behanova , Carolina Wählby , Filip Malmberg

It has been shown that for automated PAP-smear image classification, nucleus features can be very informative. Therefore, the primary step for automated screening can be cell-nuclei detection followed by segmentation of nuclei in the…

Computer Vision and Pattern Recognition · Computer Science 2018-06-26 Srishti Gautam , Harinarayan K. K. , Nirmal Jith , Anil K. Sao , Arnav Bhavsar , Adarsh Natarajan

Single-cell RNA-seq data allow the quantification of cell type differences across a growing set of biological contexts. However, pinpointing a small subset of genomic features explaining this variability can be ill-defined and…

Machine Learning · Statistics 2022-07-29 Nabeel Sarwar , Wilson Gregory , George A Kevrekidis , Soledad Villar , Bianca Dumitrascu

Applications of single-cell RNA sequencing in various biomedical research areas have been blooming. This new technology provides unprecedented opportunities to study disease heterogeneity at the cellular level. However, unique…

Genomics · Quantitative Biology 2021-10-26 Xinlei Mi , William Bekerman , Peter A. Sims , Peter D. Canoll , Jianhua Hu

Sketches are probabilistic data structures that can provide approximate results within mathematically proven error bounds while using orders of magnitude less memory than traditional approaches. They are tailored for streaming data analysis…

Data Structures and Algorithms · Computer Science 2019-03-05 Fatih Taşyaran , Kerem Yıldırır , Kamer Kaya , Mustafa Kemal Taş

Flow cytometry is a technology that rapidly measures antigen-based markers associated to cells in a cell population. Although analysis of flow cytometry data has traditionally considered one or two markers at a time, there has been…

Applications · Statistics 2010-03-30 Gyemin Lee , William Finn , Clayton Scott

In immunological studies, the characterization of small, functionally distinct cell subsets from blood and tissue is crucial to decipher system level biological changes. An increasing number of studies rely on assays that provide…

Sketching is a probabilistic data compression technique that has been largely developed in the computer science community. Numerical operations on big datasets can be intolerably slow; sketching algorithms address this issue by generating a…

Methodology · Statistics 2019-04-04 Daniel Ahfock , William J. Astle , Sylvia Richardson

Most cellular phenotypes are genetically complex. Identifying the set of genes that are most closely associated with a specific cellular state is still an open question in many cases. Here we study the transcriptional profile of cellular…

Quantitative Methods · Quantitative Biology 2024-06-21 Alda Sabalic , Victoria Moiseeva , Andres Cisneros , Oleg Deryagin , Eusebio Perdiguero , Pura Muñoz-Canoves , Jordi Garcia-Ojalvo

Single-cell RNA sequencing (scRNA-seq) technology enables systematic delineation of cellular states and interactions, providing crucial insights into cellular heterogeneity. Building on this potential, numerous computational methods have…

Genomics · Quantitative Biology 2025-11-11 Ping Xu , Zaitian Wang , Zhirui Wang , Pengjiang Li , Ran Zhang , Gaoyang Li , Hanyu Xie , Jiajia Wang , Yuanchun Zhou , Pengfei Wang

Structured high-cardinality data arises in many domains, and poses a major challenge for both modeling and inference. Graphical models are a popular approach to modeling structured data but they are unsuitable for high-cardinality…

Data Structures and Algorithms · Computer Science 2016-07-19 Branislav Kveton , Hung Bui , Mohammad Ghavamzadeh , Georgios Theocharous , S. Muthukrishnan , Siqi Sun

We propose a deep hashing framework for sketch retrieval that, for the first time, works on a multi-million scale human sketch dataset. Leveraging on this large dataset, we explore a few sketch-specific traits that were otherwise…

Computer Vision and Pattern Recognition · Computer Science 2018-04-05 Peng Xu , Yongye Huang , Tongtong Yuan , Kaiyue Pang , Yi-Zhe Song , Tao Xiang , Timothy M. Hospedales , Zhanyu Ma , Jun Guo

Single-cell technologies have revolutionized biomedical research by enabling scalable measurement of the genome, transcriptome, and proteome of multiple systems at single-cell resolution. Now widely applied to cancer models, these assays…

Genomics · Quantitative Biology 2020-05-05 Allen W Zhang , Kieran R Campbell

A critical challenge in single-cell RNA sequencing (scRNA-seq) integration is resolving the tension between eliminating batch effects and maintaining biological fidelity. While recent evidence indicates that batch effects manifest…

Machine Learning · Computer Science 2026-05-19 Xichen Yan , Zelin Zang , Changxi Chi , Jingbo Zhou , Chang Yu , Jinlin Wu , Shenghui Cheng , Fuji Yang , Jiebo Luo , Zhen Lei , Stan Z. Li

Probabilistic ideas and tools have recently begun to permeate into several fields where they had traditionally not played a major role, including fields such as numerical linear algebra and optimization. One of the key ways in which these…

Numerical Analysis · Mathematics 2016-12-20 Robert M. Gower