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Evanescent light excitation is widely used in super-resolution fluorescence microscopy to confine light and reduce background noise. Herein we propose a method of exploiting evanescent light in the context of emission. When a fluorophore is…

Biological Physics · Physics 2015-09-30 N. Bourg , C. Mayet , G. Dupuis , T. Barroca , P. Bon , S. Lécart , E. Fort , S. Lévêque-Fort

Near-diffraction limited imaging and spectroscopy in the visible on large (8-10 meter) class telescopes has proved to be beyond the capabilities of current adaptive optics technologies, even when using laser guide stars. The need for high…

Instrumentation and Methods for Astrophysics · Physics 2015-05-19 Craig Mackay , Tim D. Staley , David King , Frank Suess , Keith Weller

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

Imaging below the diffraction limit is always a public interest because of the restricted resolution of conventional imaging systems. To beat the limit, evanescent harmonics decaying in space must participate in the imaging process. Here,…

Optics · Physics 2019-10-02 Tie-Jun Huang , Li-Zheng Yin , Ya Shuang , Jiang-Yu Liu , Yunhua Tan , Pu-Kun Liu

Despite all the success with developing super-resolution imaging techniques, the Abbe limit poses a severe fundamental restriction on the resolution of far-field imaging systems based on diffraction of light. Imaging with contact…

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

Data Structures and Algorithms · Computer Science 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song

Full-field x-ray microscopy using x-ray objectives has become a mainstay of the biological and materials sciences. However, the inefficiency of existing objectives at x-ray energies above 15 keV has limited the technique to weakly absorbing…

High-resolution fMRI provides a window into the brain's mesoscale organization. Yet, higher spatial resolution increases scan times, to compensate for the low signal and contrast-to-noise ratio. This work introduces a deep learning-based 3D…

Image and Video Processing · Electrical Eng. & Systems 2024-03-20 Hongwei Bran Li , Matthew S. Rosen , Shahin Nasr , Juan Eugenio Iglesias

Fluorescence microscopy has enabled a dramatic development in modern biology by visualizing biological organisms with micrometer scale resolution. However, due to the diffraction limit, sub-micron/nanometer features are difficult to…

Image and Video Processing · Electrical Eng. & Systems 2021-03-10 Varun Mannam , Yide Zhang , Xiaotong Yuan , Scott Howard

Femtosecond interferometry combined with acousto-optical phase modulation is an effective approach to implement various types of coherent nonlinear and multidimensional spectroscopy schemes. The high sensitivity of this method has recently…

Atomic Physics · Physics 2025-02-24 Friedemann Landmesser , Nicolai Gölz , Ulrich Bangert , Tobias Sixt , Lukas Bruder

Fourier ptychography (FP) is a promising computational imaging technique that overcomes the physical space-bandwidth product (SBP) limit of a conventional microscope by applying angular diversity illuminations. However, to date, the…

Optics · Physics 2018-10-17 An Pan , Yan Zhang , Kai Wen , Maosen Li , Meiling Zhou , Junwei Min , Ming Lei , Baoli Yao

Remote focusing of light in a graded-index medium via mode interference is demonstrated using exact analytical solutions of the wave equation. Strong focusing of light occurs at extremely long distances and it revivals periodically with…

Optics · Physics 2015-12-09 Nikolai I. Petrov

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

Single-beam super-resolution microscopy, also known as superlinear microscopy, exploits the nonlinear response of fluorescent probes in confocal microscopy. The technique requires no complex purpose-built system, light field modulation, or…

Coherent diffractive imaging is unique as the only route for achieving diffraction-limited spatial resolution in the extreme ultraviolet and X-ray regions, limited only by the wavelength of the light. Recently, advances in coherent short…

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

Fluorescence imaging is an essential diagnostic tool in many fields, but diffraction-limited optical imaging at depth is limited by scattering. Here, we present a method based on multiple random illuminations, combined with a computational…

Optics · Physics 2026-03-30 Lei Zhu , Tengfei Wu , Bernhard Rauer , Hilton B. de Aguiar , Sylvain Gigan

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

Far-field chemical microscopy providing molecular electronic or vibrational fingerprint information opens a new window for the study of three-dimensional biological, material, and chemical systems. Chemical microscopy provides a…

Optics · Physics 2023-06-06 Mingwei Tang , Yubing Han , Danchen Jia , Qing Yang , Ji-Xin Cheng