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We present a computational live bacteria detection system that periodically captures coherent microscopy images of bacterial growth inside a 60 mm diameter agar-plate and analyzes these time-lapsed holograms using deep neural networks for…

During the development of vaccines, bacterial colony forming units (CFUs) are counted in order to quantify the yield in the fermentation process. This manual task is time-consuming and error-prone. In this work we test multiple segmentation…

Computer Vision and Pattern Recognition · Computer Science 2020-09-03 Thomas Beznik , Paul Smyth , Gaël de Lannoy , John A. Lee

A Rapid and cost-effective method for detecting bacterial cells on surfaces is critical to protect public health from various aspects, including food safety, clinical hygiene, and pharmacy quality. Herein, we first established an optical…

Quantitative Methods · Quantitative Biology 2024-03-13 Yuzhen Zhang , Zili Gao , Lili He

We propose for the first time an E. coli bacteria sensor based on the evanescent field of the fundamental mode of a suspended-core terahertz fiber. The sensor is capable of E. coli detection at concentrations in the range of 104-109 cfu/ml.…

Biological Physics · Physics 2015-06-03 Anna Mazhorova , Andrey Markov , Andy Ng , Raja Chinnappan , Mohammed Zourob , Maksim Skorobogatiy

A hallmark of bacterial populations cultured in vitro is their homogeneity of growth, where the majority of cells display identical growth rate, cell size and content. Recent insights, however, have revealed that even cells growing in…

Quantitative Methods · Quantitative Biology 2022-06-07 Santosh Pandey , Yunsoo Park , Ankita Ankita , Gregory J. Phillips

Bacterial infectious diseases are a major threat to human health. Timely and sensitive pathogenic bacteria detection is crucial in identifying the bacterial contaminations and preventing the spread of infectious diseases. Due to limitations…

Diagnosis of fungal infections can rely on microscopic examination, however, in many cases, it does not allow unambiguous identification of the species due to their visual similarity. Therefore, it is usually necessary to use additional…

Computer Vision and Pattern Recognition · Computer Science 2020-10-14 Bartosz Zieliński , Agnieszka Sroka-Oleksiak , Dawid Rymarczyk , Adam Piekarczyk , Monika Brzychczy-Włoch

Raman spectroscopy is a promising tool for microbial identification, yet its implementation in microbiology and clinical workflow is still restricted due to the accompanying additional preparation required to focus on microbial signals.…

Quantitative Methods · Quantitative Biology 2026-03-19 Jeong Hee Kim , Jia Dong , Marissa Morales , Loza Tadesse

Preliminary diagnosis of fungal infections can rely on microscopic examination. However, in many cases, it does not allow unambiguous identification of the species by microbiologist due to their visual similarity. Therefore, it is usually…

Computer Vision and Pattern Recognition · Computer Science 2020-09-09 Bartosz Zieliński , Agnieszka Sroka-Oleksiak , Dawid Rymarczyk , Adam Piekarczyk , Monika Brzychczy-Włoch

We introduce a new image cytometer design for detection of very small particulate and demonstrate its capability in water analysis. The device is a compact microscope composed of off the shelf components, such as a light emitting diode…

Optics · Physics 2016-02-17 Juan Miguel Pérez , Marc Jofre , Pedro Martínez , Valerio Pruneri

Bacterial colony enumeration is an essential stage in microbiological research, allowing susceptibility to antibiotics assessment, monitoring of the environment, and clinical diagnostics. Traditional manual counting methods are costly and…

Quantitative Methods · Quantitative Biology 2025-05-28 Areesha Rehman , Zikria Saleem , Jarrar Amjad , Syed Rehan Shah , Kamran Siddique

Colony-forming unit (CFU) detection is critical in pharmaceutical manufacturing, serving as a key component of Environmental Monitoring programs and ensuring compliance with stringent quality standards. Manual counting is labor-intensive…

Computer Vision and Pattern Recognition · Computer Science 2026-02-25 Subhra Jyoti Mandal , Lara Rachidi , Puneet Jain , Matthieu Duvinage , Sander W. Timmer

Developing rapid methods for pathogen detection and growth monitoring at low cell and analyte concentrations is an important goal, which numerous technologies are working towards solving. Rapid biosensors have already made a dramatic impact…

Our work targets automated analysis to quantify the growth dynamics of a population of bacilliform bacteria. We propose an innovative approach to frame-sequence tracking of deformable-cell motion by the automated minimization of a new,…

Computer Vision and Pattern Recognition · Computer Science 2022-07-12 Sorena Sarmadi , James J. Winkle , Razan N. Alnahhas , Matthew R. Bennett , Krešimir Josić , Andreas Mang , Robert Azencott

Electrical capacitance tomography (ECT) is a nonoptical imaging technique in which a map of the interior permittivity of a volume is estimated by making capacitance measurements at its boundary and solving an inverse problem. While previous…

Computer Vision and Pattern Recognition · Computer Science 2023-12-05 Manar Abdelatty , Joseph Incandela , Kangping Hu , Joseph W. Larkin , Sherief Reda , Jacob K. Rosenstein

Recognition and analysis of Diabetic Foot Ulcers (DFU) using computerized methods is an emerging research area with the evolution of image-based machine learning algorithms. Existing research using visual computerized methods mainly focuses…

Image and Video Processing · Electrical Eng. & Systems 2020-02-11 Manu Goyal , Neil Reeves , Satyan Rajbhandari , Naseer Ahmad , Chuan Wang , Moi Hoon Yap

Automated recognition and classification of bacteria species from microscopic images have significant importance in clinical microbiology. Bacteria classification is usually carried out manually by biologists using different shapes and…

Quantitative Methods · Quantitative Biology 2019-12-19 Muhammed Talo

The spread of microbial infections is governed by the self-organization of bacteria on surfaces. Limitations of live imaging techniques make collective behaviors in clinically relevant systems challenging to quantify. Here, novel…

Biological Physics · Physics 2024-07-19 Vincent Hickl , Abid Khan , René M. Rossi , Bruno F. B. Silva , Katharina Maniura-Weber

Sepsis is a life-threatening condition which requires rapid diagnosis and treatment. Traditional microbiological methods are time-consuming and expensive. In response to these challenges, deep learning algorithms were developed to identify…

Strategies for in-liquid micro-organism detection are crucial for the clinical and pharmaceutical industries. While Raman spectroscopy is a promising label-free technique for micro-organism detection, it remains challenging due to the weak…

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