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We propose a single-shot quantitative differential phase contrast (DPC) method with polarization multiplexing illumination. In the illumination module of our system, the programmable LED array is divided into four quadrants and covered with…

Optics · Physics 2023-07-19 Siying Liu , Chuanjian Zheng , Qun Hao , Xin Li , Shaohui Zhang

Fourier ptychographic microscopy (FPM) is a recently developed imaging modality that uses angularly varying illumination to extend a system performance beyond the limit defined by its optical elements. The FPM technique applies a novel…

Optics · Physics 2014-05-02 Xiaoze Ou , Roarke Horstmeyer , Changhuei Yang , Guoan Zheng

Phase-contrast microscopy converts the optical phase introduced by transparent, unlabeled specimens into modulation in the intensity image. Modern phase imaging techniques are capable of quantifying phase shift at each point in the field of…

Analyser-Based Phase-Contrast imaging (ABPCI) is a phase-contrast imaging method that produces high contrast images of weakly absorbing materials. However, it is only sensitive to phase variations lying in the diffraction plane of the…

Medical Physics · Physics 2021-02-11 M. C. Chalmers , M. J. Kitchen , K. Uesugi , G. Falzon , P. Quin , K. M. Pavlov

In this paper we present a method to robustly evaluate the quantitative accuracy of various tomographic phase microscopy (TPM) methods with a multiple scattering 3D-printed microphantom with known geometry and refractive index distribution.…

We present differential phase-contrast optical coherence tomography (DPC-OCT) with two transversally separated probing beams to sense phase gradients in various directions by employing a rotatable Wollaston prism. In combination with a…

Optics · Physics 2011-11-11 Bettina Heise , David Stifter

This paper presents a microscopic imaging technique that uses variable-angle illumination to recover the complex polarimetric properties of a specimen at high resolution and over a large field-of-view. The approach extends Fourier…

In situ electron microscopy is a key tool for understanding the mechanisms driving novel phenomena in 2D structures. Unfortunately, due to various practical challenges, technologically relevant 2D heterostructures prove challenging to…

We deal with the photoacoustic imaging modality using dielectric nanoparticles as contrast agents. Exciting the heterogeneous tissue, localized in a bounded domain $\Omega$, with an electromagnetic wave, at a given incident frequency,…

Analysis of PDEs · Mathematics 2020-03-09 Ahcene Ghandriche , Mourad Sini

Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging.…

Fluorescence microscopy (FM) imaging is a fundamental technique for observing live cell division, one of the most essential processes in the cycle of life and death. Observing 3D live cells requires scanning through the cell volume while…

Image and Video Processing · Electrical Eng. & Systems 2026-01-28 Chenwei Wang , Zhaoke Huang , Zelin Li , Wenqi Zhu

Conventional fiber-bundle-based endoscopes allow minimally invasive imaging through flexible multi-core fiber (MCF) bundles by placing a miniature lens at the distal tip and using each core as an imaging pixel. In recent years, lensless…

Optics · Physics 2024-02-02 Gil Weinberg , Munkyu Kang , Wonjun Choi , Wonshik Choi , Ori Katz

The visualization of pure phase objects by wavefront sensing has important applications ranging from surface profiling to biomedical microscopy, and generally requires bulky and complicated setups involving optical spatial filtering,…

Optics · Physics 2023-08-29 Jianing Liu , Hao Wang , Yuyu Li , Lei Tian , Roberto Paiella

Ptychography is an imaging technique which involves a sample being illuminated by a coherent, localized probe of illumination. When the probe interacts with the sample, the light is diffracted and a diffraction pattern is detected. Then the…

Numerical Analysis · Mathematics 2023-01-31 Mark Iwen , Michael Perlmutter , Mark Philip Roach

Dielectric tensor tomography is an imaging technique for mapping three-dimensional distributions of dielectric properties in transparent materials. This work introduces an enhanced illumination strategy employing a micro-electromechanical…

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

Optics · Physics 2025-01-22 Jaromír Běhal , Miroslav Ježek

Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

We present geometric-phase microscopy allowing a multipurpose quantitative phase imaging in which the ground-truth phase is restored by quantifying the phase retardance. The method uses broadband spatially incoherent light that is…

Two-dimensional topological insulators are central to our understanding of the connection between topological symmetries in a material and its band electronics. Within this class of materials, a breadth of complex quantum behaviors, such as…

In the last five decades, iterative phase retrieval methods draw large amount of interest across the research community as a non-interferometric approach to recover quantitative phase distributions from one (or more) intensity measurement.…

Optics · Physics 2020-07-21 Nathaniel Hai , Joseph Rosen