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We are frequently faced with a large collection of antibodies, and want to select those with highest affinity for their cognate antigen. When developing a first-line therapeutic for a novel pathogen, for instance, we might look for such…

Populations and Evolution · Quantitative Biology 2021-01-27 Duncan K. Ralph , Frederick A. Matsen

The human immune system depends on a highly diverse collection of antibody-making B cells. B cell receptor sequence diversity is generated by a random recombination process called "rearrangement" forming progenitor B cells, then a Darwinian…

Populations and Evolution · Quantitative Biology 2017-02-08 Duncan K. Ralph , Frederick A. Matsen

B-cell repertoires are characterized by a diverse set of receptors of distinct specificities generated through two processes of somatic diversification: V(D)J recombination and somatic hypermutations. B cell clonal families stem from the…

Populations and Evolution · Quantitative Biology 2024-03-19 Natanael Spisak , Gabriel Athènes , Thomas Dupic , Thierry Mora , Aleksandra M. Walczak

One of the key phenomena in the adaptive immune response to infection and immunization is affinity maturation, during which antibody genes are mutated and selected, typically resulting in a substantial increase in binding affinity to the…

Quantitative Methods · Quantitative Biology 2013-03-05 Thomas B. Kepler

B cell receptors (BCRs) play a crucial role in recognizing and fighting foreign antigens. High-throughput sequencing enables in-depth sampling of the BCRs repertoire after immunization. However, only a minor fraction of BCRs actively…

Populations and Evolution · Quantitative Biology 2023-12-22 Maria Francesca Abbate , Thomas Dupic , Emmanuelle Vigne , Melody A. Shahsavarian , Aleksandra M. Walczak , Thierry Mora

Fundamental to quantitative characterization of the B cell receptor repertoire is clonal diversity - the number of distinct somatically recombined receptors present in the repertoire and their relative abundances, defining the search space…

Quantitative Methods · Quantitative Biology 2017-08-30 William DeWitt , Paul Lindau , Thomas Snyder , Marissa Vignali , Ryan Emerson , Harlan Robins

While clustering is ubiquitously used across science and industry, uncertainty in cluster assignments is rarely quantified with rigorous guarantees. We propose a novel conformal inference framework for clustering that returns confidence…

Methodology · Statistics 2026-04-13 YoonHaeng Hur , Anirban Nath , Genevera Allen

B cells develop high affinity receptors during the course of affinity maturation, a cyclic process of mutation and selection. At the end of affinity maturation, a number of cells sharing the same ancestor (i.e. in the same "clonal family")…

Biomolecules · Quantitative Biology 2018-11-21 Amrit Dhar , Kristian Davidsen , Frederick A. Matsen , Vladimir N. Minin

Diverse T and B cell repertoires play an important role in mounting effective immune responses against a wide range of pathogens and malignant cells. The number of unique T and B cell clones is characterized by T and B cell receptors (TCRs…

Quantitative Methods · Quantitative Biology 2026-05-12 Lucas Böttcher , Sascha Wald , Tom Chou

Motivation: Identifying interaction clusters of large gene regulatory networks (GRNs) is critical for its further investigation, while this task is very challenging, attributed to data noise in experiment data, large scale of GRNs, and…

Machine Learning · Computer Science 2019-10-22 Yu Chen , Yuanyuan Yang , Yaochu Jin , Xiufen Zou

High-throughput sequencing of B- and T-cell receptors makes it possible to track immune repertoires across time, in different tissues, and in acute and chronic diseases or in healthy individuals. However, quantitative comparison between…

Quantitative Methods · Quantitative Biology 2020-11-20 Maximilian Puelma Touzel , Aleksandra M. Walczak , Thierry Mora

T-cell receptor (TCR) repertoire data contain information about infections that could be used in disease diagnostics and vaccine development, but extracting that information remains a major challenge. Here we developed a statistical…

The collection of immunoglobulin genes in an individual's germline, which gives rise to B cell receptors via recombination, is known to vary significantly across individuals. In humans, for example, each individual has only a fraction of…

Populations and Evolution · Quantitative Biology 2019-07-26 Duncan K. Ralph , Frederick A. Matsen

A model of bit-strings, that uses the technique of multi-spin coding, was previously used to study the time evolution of B-cell clone repertoire, in a paper by Lagreca, Almeida and Santos. In this work we extend that simplified model to…

Populations and Evolution · Quantitative Biology 2017-07-31 Alexandre de Castro

Large-scale source-code clone detection is a challenging task. In our previous work, we proposed an approach (SSCD) that leverages artificial neural networks and approximates nearest neighbour search to effectively and efficiently locate…

Software Engineering · Computer Science 2024-02-13 Gul Aftab Ahmed , James Vincent Patten , Yuanhua Han , Guoxian Lu , David Gregg , Jim Buckley , Muslim Chochlov

Cluster analysis methods are used to identify homogeneous subgroups in a data set. In biomedical applications, one frequently applies cluster analysis in order to identify biologically interesting subgroups. In particular, one may wish to…

Methodology · Statistics 2016-09-23 Sheila Gaynor , Eric Bair

Immunology is the emerging research area which deals with the study of the immune system in any living organism. It is modelled through various computational and mathematical models to deal with the problem facing while to boost the immune…

Cell Behavior · Quantitative Biology 2019-06-21 Gajendra Pratap Singh , Madhuri Jha

Understanding the base pairing of an RNA sequence provides insight into its molecular structure.By mining suboptimal sampling data, RNAprofiling 1.0 identifies the dominant helices in low-energy secondary structures as features, organizes…

Biomolecules · Quantitative Biology 2023-03-29 Forrest Hurley , Christine Heitsch

T-cell receptors (TCR) are key proteins of the adaptive immune system, generated randomly in each individual, whose diversity underlies our ability to recognize infections and malignancies. Modeling the distribution of TCR sequences is of…

Quantitative Methods · Quantitative Biology 2020-07-01 Giulio Isacchini , Zachary Sethna , Yuval Elhanati , Armita Nourmohammad , Aleksandra M. Walczak , Thierry Mora

Single-cell RNA sequencing (scRNA-seq) enables researchers to analyze gene expression at single-cell level. One important task in scRNA-seq data analysis is unsupervised clustering, which helps identify distinct cell types, laying down the…

Genomics · Quantitative Biology 2023-12-29 Weikang Jiang , Jinxian Wang , Jihong Guan , Shuigeng Zhou
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