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Super-Resolution Microscopy (SRM) is emerging as a powerful and innovative tool for imaging, characterizing, and understanding the structure of Extracellular Vesicles (EVs). By addressing the need for single-particle analysis with the high…

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

To be able to resolve molecular-clusters it is crucial to access vital informations (such as, molecule density and cluster-size) that are key to understand disease progression and the underlying mechanism. Traditional single-molecule…

Quantitative Methods · Quantitative Biology 2021-01-27 Partha Pratim Mondal

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

Subcellular Processes · Quantitative Biology 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…

Diagnostics capable of interrogating dynamics in harsh environments such as plasma have remained essentially unchanged in recent decades. Developments in advanced microscopy techniques will improve our understanding of the physics involved…

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

Super-resolution microscopy has revolutionized optical fluorescence imaging by improving 3D resolution by 1-2 orders of magnitude. While different methods can successfully increase the resolution, all methods share significant differences…

Biological Physics · Physics 2015-01-26 Thomas Pengo , Nicolas Olivier , Suliana Manley

Super-resolution (SR) is a technique that allows increasing the resolution of a given image. Having applications in many areas, from medical imaging to consumer electronics, several SR methods have been proposed. Currently, the best…

Computer Vision and Pattern Recognition · Computer Science 2019-12-02 Marija Vella , João F. C. Mota

Super-resolution is the process of obtaining a high-resolution image from one or more low-resolution images. Single image super-resolution (SISR) and multi-frame super-resolution (MFSR) methods have been evolved almost independently for…

Computer Vision and Pattern Recognition · Computer Science 2023-03-07 Mohammad Mahdi Afrasiabi , Reshad Hosseini , Aliazam Abbasfar

When a single molecule is detected in a wide-field microscope, the image approximates the point spread function of the system. However, as the distribution of molecules becomes denser and their images begin to coincide, existing solutions…

Optics · Physics 2013-09-02 Anthony Barsic , Ginni Grover , Rafael Piestun

Single molecule localization microscopy (SMLM) techniques enable imaging biological samples well beyond the diffraction limit of light, but they vary significantly in their spatial and temporal resolutions. High-order statistical analysis…

Biological Physics · Physics 2016-03-15 Shachar Schidorsky , Xiyu Yi , Yair Razvag , Yonatan Golan , Shimon Weiss , Eilon Sherman

Raman microscopy is a valuable tool for detecting physical and chemical properties of a sample material. When probing nanomaterials or nanocomposites the spatial resolution of Raman microscopy is not always adequate as it is limited by the…

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

Formation, maintenance and physiology of high-density protein-enriched organized nanodomains, first observed in electron microscopy images, remains challenging to investigate due to their small sizes. However, these regions regulate…

Quantitative Methods · Quantitative Biology 2023-12-29 Pierre Parutto , Jennifer Heck , Martin Heine , David Holcman

The nanoscale interaction between single emitters and plasmonic structures is traditionally studied by relying on near-perfect, deterministic, nanoscale-control. This approach is ultra-low throughput thus rendering systematic studies…

Chemical Physics · Physics 2019-04-29 Lisa Saemisch , Matz Liebel , Niek van Hulst

The primary objective of this research was to enhance the quality of semantic segmentation in cytology images by incorporating super-resolution (SR) architectures. An additional contribution was the development of a novel dataset aimed at…

Super-resolution (SR), the process of obtaining high-resolution images from one or more low-resolution observations of the same scene, has been a very popular topic of research in the last few decades in both signal processing and image…

Image and Video Processing · Electrical Eng. & Systems 2022-02-25 Bahattin Can Maral

Single image super-resolution (SISR) is a very popular topic nowadays, which has both research value and practical value. In daily life, we crop a large image into sub-images to do super-resolution and then merge them together. Although…

Computer Vision and Pattern Recognition · Computer Science 2019-07-31 Junyu , Wang , Rong Song

Single image super-resolution (SISR) is the process of obtaining one high-resolution version of a low-resolution image by increasing the number of pixels per unit area. This method has been actively investigated by the research community,…

Image and Video Processing · Electrical Eng. & Systems 2022-10-11 George Corrêa de Araújo , Helio Pedrini
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