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Single-molecule approaches to biology offer a powerful new vision to elucidate the mechanisms that underpin the functioning of living cells. However, conventional optical single molecule spectroscopy techniques such as F\"orster…

Achieving high-throughput, comprehensive analysis of single nanoparticles to determine their size, shape, and composition is essential for understanding particle heterogeneity with applications ranging from drug delivery to environmental…

The nanoscale interaction between single emitters and plasmonic structures is traditionally studied by relying on near-perfect, deterministic, nanoscale-control. This approach is ultra-low throughput thus rendering systematic studies…

Chemical Physics · Physics 2019-04-29 Lisa Saemisch , Matz Liebel , Niek van Hulst

Current optical detection schemes for single molecules require light absorption, either to produce fluorescence or direct absorption signals. This severely limits the range of molecules that can be detected, because most molecules are…

Chemical Physics · Physics 2012-07-06 Peter Zijlstra , Pedro M. R. Paulo , Michel Orrit

High-Q optical resonators allow label-free detection of individual nanoparticles through perturbation of optical signatures but have practical limitations due to reliance on random diffusion to deliver particles to the sensing region. We…

Optics · Physics 2016-10-03 Jeewon Suh , Kewen Han , Christopher W Peterson , Gaurav Bahl

High-speed tracking of single particles is a gateway to understanding physical, chemical, and biological processes at the nanoscale. It is also a major experimental challenge, particularly for small, nanometer-scale particles. Although…

Silicon nanochannel field effect transistor (FET) biosensors are one of the most promising technologies in the development of highly sensitive and label-free analyte detection for cancer diagnostics. With their exceptional electrical…

Quantitative Methods · Quantitative Biology 2014-01-07 Pritiraj Mohanty , Yu Chen , Xihua Wang , Mi K. Hong , Carol L. Rosenberg , David T. Weaver , Shyamsunder Erramilli

The movement of molecules inside living cells is a fundamental feature of biological processes. The ability to both observe and analyse the details of molecular diffusion in vivo at the single molecule and single cell level can add…

Quantitative Methods · Quantitative Biology 2012-11-21 Alex Robson , Kevin Burrage , Mark Leake

Single molecules are nanoscale thermodynamic systems with few degrees of freedom. Thus, the knowledge of their entropy can reveal the presence of microscopic electron transfer dynamics, that are difficult to observe otherwise. Here, we…

Mesoscale and Nanoscale Physics · Physics 2021-12-08 Eugenia Pyurbeeva , Chunwei Hsu , David Vogel , Christina Wegeberg , Marcel Mayor , Herre van der Zant , Jan A. Mol , Pascal Gehring

Biological systems have been theoretically predicted to support phononic topological wave-modes, similar to the ones existing in meta-materials. The existing methods to measure these modes, however cannot be applied to biological systems;…

Biological Physics · Physics 2023-03-03 Ssu-Ying Chen , Arooj Aslam , David J. Apigo , Sagnik Basuray , Camelia Prodan

Single-molecule sensing is at the core of modern biophysics and nanoscale science, from revolutionizing healthcare through rapid, low-cost sequencing to understanding various physical, chemical, and biological processes at their most basic…

Mesoscale and Nanoscale Physics · Physics 2018-04-10 Daniel Gruss , Alex Smolyanitsky , Michael Zwolak

Molecular motor gliding motility assays based on myosin/actin or kinesin/microtubules are of interest for nanotechnology applications ranging from cargo-trafficking in lab-on-a-chip devices to novel biocomputation strategies. Prototype…

Mesoscale and Nanoscale Physics · Physics 2023-05-16 M. Sanchez Miranda , R. Lyttleton , P. H. Siu , S. Diez , H. Linke , A. P. Micolich

Ultrafast, high quantum efficiency single photon detectors are among the most sought-after elements in modern quantum optics and quantum communication. High photon detection efficiency is essential for scalable measurement-based quantum…

Optics · Physics 2015-05-30 W. Pernice , C. Schuck , O. Minaeva , M. Li , G. N. Goltsman , A. V. Sergienko , H. X. Tang

Sensors that are able to detect and track single unlabelled biomolecules are an important tool both to understand biomolecular dynamics and interactions at nanoscale, and for medical diagnostics operating at their ultimate detection limits.…

Optics · Physics 2016-11-15 N. P. Mauranyapin , L. S. Madsen , M. A. Taylor , M. Waleed , W. P. Bowen

Single particle-resolved fluorescence imaging is an enabling technology in cold-atom physics. However, so far, this technique was not available for nanophotonic atom-light interfaces. Here, we image single atoms that are trapped and…

Label-free biosensors enable the monitoring of biomolecular interactions in real-time, which is key to the analysis of the binding characteristics of biomolecules. While refractometric optical biosensors are sensitive and well-established,…

Time course measurement of single molecules on a cell surface provides detailed information on the dynamics of the molecules, which is otherwise inaccessible. To extract the quantitative information, single particle tracking (SPT) is…

Quantitative Methods · Quantitative Biology 2016-01-06 Shunsuke Teraguchi , Yutaro Kumagai

Controlling quantum light-matter interactions at scales smaller than the diffraction limit at the single quantum emitter level is a critical challenge to the goal of advancing quantum technologies. We introduce a novel material platform…

Recent technological advances in cutting-edge ultrasensitive fluorescence microscopy have allowed single-molecule imaging experiments in living cells across all three domains of life to become commonplace. Single-molecule live-cell data is…

Biomolecules · Quantitative Biology 2015-04-15 Mark Leake

Label-free biosensors are important tools for clinical diagnostics and for studying biology at the single molecule level. The development of optical label-free sensors has allowed extreme sensitivity, but can expose the biological sample to…