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The challenge of imaging low-density objects in an electron microscope without causing beam damage is significant in modern TEM. This is especially true for life science imaging, where the sample, rather than the instrument, still…

Optics · Physics 2025-01-29 Francisco Vega Ibáñez , Jo Verbeeck

X-ray phase-contrast imaging has the potential to improve image contrast with lower dose by probing an object's refractive properties as well as its absorptive properties. To reconstruct a phase-contrast image from a raw dataset, a phase…

Medical Physics · Physics 2025-08-19 Giavanna Jadick , Patrick La Rivière

Holographic coherent X-ray imaging enables nanoscale imaging of biological cells and tissues, rendering both phase and absorption contrast, i.e. real and imaginary parts of the refractive index. Unlike the standard model, which assumes a…

Analysis of PDEs · Mathematics 2026-05-29 Thorsten Hohage , Milad Karimi , Björn Müller

Single particle cryo-electron microscopy (EM) is an increasingly popular method for determining the 3-D structure of macromolecules from noisy 2-D images of single macromolecules whose orientations and positions are random and unknown. One…

Computer Vision and Pattern Recognition · Computer Science 2017-04-11 Roy R. Lederman , Amit Singer

Fraunhofer diffraction is a well-known phenomenon achieved with most wavelength even without lens. A single-shot intensity measurement of diffraction is generally considered inadequate to reconstruct the original light field, because the…

Image and Video Processing · Electrical Eng. & Systems 2019-12-04 An-Dong Xiong , Xiao-Peng Jin , Wen-Kai Yu , Qing Zhao

Proposals to determine biomolecular structures from diffraction experiments using femtosecond X-ray free-electron laser (XFEL) pulses involve a conflict between the incident brightness required to achieve diffraction-limited atomic…

Optics · Physics 2015-05-19 Harry M. Quiney , Keith A. Nugent

Electronic wave functions of planar molecules can be reconstructed via inverse Fourier transform of angle-resolved photoelectron spectroscopy (ARPES) data, provided the phase of the electron wave in the detector plane is known. Since the…

Other Condensed Matter · Physics 2016-09-23 Pavel Kliuiev , Tatiana Latychevskaia , Juerg Osterwalder , Matthias Hengsberger , Luca Castiglioni

By analyzing ``exact'' theoretical results from solving the time-dependent Schr\"odinger equation of atoms in few-cycle laser pulses, we established the general conclusion that differential elastic scattering and photo-recombination cross…

Atomic Physics · Physics 2008-04-04 Toru Morishita , Anh-Thu Le , Zhangjin Chen , C. D. Lin

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Fast frame-rates are desirable in scanning transmission electron microscopy for a number of reasons: controlling electron beam dose, capturing in-situ events or reducing the appearance of scan distortions. Whilst several strategies exist…

Instrumentation and Detectors · Physics 2023-08-09 Jonathan J. P Peters , Tiarnan Mullarkey , James A. Gott , Elizabeth Nelson , Lewys Jones

Magnetic resonance imaging (MRI) reconstruction is a fundamental task aimed at recovering high-quality images from undersampled or low-quality MRI data. This process enhances diagnostic accuracy and optimizes clinical applications. In…

Image and Video Processing · Electrical Eng. & Systems 2025-03-11 Xiaoyan Kui , Zijie Fan , Zexin Ji , Qinsong Li , Chengtao Liu , Weixin Si , Beiji Zou

One of the most powerful approaches to imaging at the nanometer or subnanometer length scale is coherent diffraction imaging using X-ray sources. For amorphous (non-crystalline) samples, the raw data can be interpreted as the modulus of the…

Numerical Analysis · Mathematics 2020-04-02 Alexander Barnett , Charles L. Epstein , Leslie Greengard , Jeremy Magland

Super-resolution reconstruction techniques entail the utilization of software algorithms to transform one or more sets of low-resolution images captured from the same scene into high-resolution images. In recent years, considerable…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Hao Yan , Zixiang Wang , Zhengjia Xu , Zhuoyue Wang , Zhizhong Wu , Ranran Lyu

Understanding complex biological macromolecules, especially proteins, is vital for grasping their diverse chemical functions with direct impact in biology and pharmacology. While techniques like X-ray crystallography and cryo-electron…

Biomolecules · Quantitative Biology 2024-04-12 S. H. Mejias , A. L. Cortajarena , R. Mincigrucci , C. Svetina , C. Masciovecchio

Lensless imaging is an elegant approach to high-resolution microscopy, which is rapidly gaining popularity in applications where imaging optics are problematic. However, current lensless imaging methods require objects to be placed within a…

Short wavelength Free-Electron Lasers (FELs) are the newest light sources available to scientists to probe a wide range of phenomena, with chemical, physical and biological applications, using soft and hard X-rays. These sources include the…

There is a growing interest in reconstructing the density matrix of photoelectron wavepackets, in particular in complex systems where decoherence can be introduced either by a partial measurement of the system or through coupling with a…

Compact laboratory-scale X-ray sources still rely on the same fundamental principles as in the first X-ray tubes developed more than a century ago. In recent years, significant research and development have focused on large-scale X-ray…

Femtosecond electron beams with complex modulation play a crucial role in applications such as X-ray Free Electron Lasers (XFELs) and plasma wakefield accelerators. However, diagnostics for the electron beam current profile still face…

Accelerator Physics · Physics 2026-05-08 Zixiao Guo , Ke Feng , Zhiheng Lou , Guiyao Wang , Wentao Wang , Ruxin Li

Ultrafast diffraction imaging is a powerful tool to retrieve the geometric structure of gas-phase molecules with combined picometre spatial and attosecond temporal resolution. However, structural retrieval becomes progressively difficult…

Chemical Physics · Physics 2021-10-13 Xinyao Liu , Kasra Amini , Aurelien Sanchez , Blanca Belsa , Tobias Steinle , Jens Biegert
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