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Traditional microbial detection methods often rely on the overall property of microbial cultures and cannot resolve individual growth event at high spatiotemporal resolution. As a result, they require bacteria to grow to confluence and then…

Ptychography, a special case of the phase retrieval problem, is a popular method in modern imaging. Its measurements are based on the shifts of a locally supported window function. In general, direct recovery of an object from such…

Numerical Analysis · Mathematics 2020-10-28 Anton Forstner , Felix Krahmer , Oleh Melnyk , Nada Sissouno

Tilting planar samples for multi-zone-axes observation is a routine procedure in electron microscopy. However, this process invariably introduces optical path differences in the electron beam across different sample positions, significantly…

Materials Science · Physics 2024-08-22 Jizhe Cui , Yi Zheng , Kang Sun , Wenfeng Yang , Haozhi Sha , Rong Yu

X-ray Ptychography is an advanced computational microscopy technique which is delivering exceptionally detailed quantitative imaging of biological and nanotechnology specimens. However coarse parametrisation in propagation distance,…

Image and Video Processing · Electrical Eng. & Systems 2021-10-20 Francesco Guzzi , George Kourousias , Fulvio Billè , Roberto Pugliese , Alessandra Gianoncelli , Sergio Carrato

We introduce a probabilistic approach to ptychographic reconstruction in computational imaging. Ptychography is an imaging method where the complex amplitude of an object is estimated from a sequence of diffraction measurements. We…

Applications · Statistics 2025-04-09 Hajime Ueda , Shun Katakami , Masato Okada

Ptychography has risen as a reference X-ray imaging technique: it achieves resolutions of one billionth of a meter, macroscopic field of view, or the capability to retrieve chemical or magnetic contrast, among other features. A…

Optimization and Control · Mathematics 2019-06-10 Huibin Chang , Pable Enfedaque , Stefano Marchesini

Lensless on-chip microscopy has shown great potential for biomedical imaging due to its large-area and high-throughput imaging capabilities. By combining the pixel super-resolution (PSR) technique, it can improve the resolution beyond the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-06 Xuyang Chang , Shaowei Jiang , Yongcun Hu , Liheng Bian

We report on a new x-ray imaging method, which combines the high spatial resolution of coherent diffraction imaging with the ability of dark field microscopy to map grains within thick polycrystalline specimens. An x-ray objective serves to…

Instrumentation and Detectors · Physics 2020-07-15 Anders Filsøe Pedersen , Virginie Chamard , Carsten Detlefs , Tao Zhou , Dina Carbone , Henning Friis Poulsen

Single-shot ptychography is a quantitative phase imaging method wherein overlapping beams of light arranged in a grid pattern simultaneously illuminate a sample, allowing a full ptychographic dataset to be collected in a single shot. It is…

Coherent diffractive imaging is unique as the only route for achieving diffraction-limited spatial resolution in the extreme ultraviolet and X-ray regions, limited only by the wavelength of the light. Recently, advances in coherent short…

Many nano and quantum devices, with their sizes often spanning from millimeters down to sub-nanometer, have intricate low-dimensional, non-uniform, or hierarchical structures on surfaces and interfaces. Since their functionalities are…

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

The measurement of optical ultrafast laser pulses is done indirectly because the required bandwidth to measure these pulses exceeds the bandwidth of current electronics. As a result, this measurement problem is often posed as a 1-D phase…

Optics · Physics 2023-04-28 Daniel J Kane , Andrei B. Vakhtin

Ptychography, a coherent diffraction imaging technique, has become an indispensable tool in materials characterization, biological imaging, and nanostructure analysis due to its capability for high-resolution, lensless reconstruction of…

Electron ptychography has recently achieved unprecedented resolution, offering valuable insights across diverse material systems, including in three dimensions. However, high-quality ptychographic reconstruction is computationally expensive…

Materials Science · Physics 2025-08-07 Chia-Hao Lee , Steven E. Zeltmann , Dasol Yoon , Desheng Ma , David A. Muller

Optical scattering presents a major obstacle to high resolution imaging in biological tissue and other turbid media. Conventional photoacoustic imaging can partially overcome this obstacle, enabling imaging of optical absorption in the…

Ptychography, a form of Coherent Diffractive Imaging, is used with short wavelengths (e.g. X-rays, electron beams) to achieve high-resolution image reconstructions. One of the limiting factors for the reconstruction quality is the accurate…

Image and Video Processing · Electrical Eng. & Systems 2018-04-02 P. Dwivedi , A. P. Konijnenberg , S. F. Pereira , H. P. Urbach

We report a new lensless microscopy configuration by integrating the concepts of transverse translational ptychography and defocus multi-height phase retrieval. In this approach, we place a tilted image sensor under the specimen for…

Image and Video Processing · Electrical Eng. & Systems 2021-11-03 Shaowei Jiang , Chengfei Guo , Patrick Hu , Derek Hu , Pengming Song , Tianbo Wang , Zichao Bian , Zibang Zhang , Guoan Zheng

In Fourier ptychography, multiple low resolution images are captured and subsequently combined computationally into a high-resolution, large-field of view micrograph. A theoretical image-formation model based on the assumption of plane-wave…

Optics · Physics 2022-06-22 Tomas Aidukas , Lars Loetgering , Andrew Robert Harvey

Fourier ptychographic microscopy enables gigapixel-scale imaging, with both large field-of-view and high resolution. Using a set of low-resolution images that are recorded under varying illumination angles, the goal is to computationally…

Signal Processing · Electrical Eng. & Systems 2018-03-13 Emrah Bostan , Mahdi Soltanolkotabi , David Ren , Laura Waller