Related papers: Combining fluorescence fluctuations and photobleac…
Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure…
The practice of measuring galaxy distances from their spatial fluctuations in surface brightness is now a decade old. While several past articles have included some review material, this is the first intended as a comprehensive review of…
Determining the number of fluorescent entities that are coupled to a given molecule (DNA, protein, etc.) is a key point of numerous biological studies, especially those based on a single molecule approach. Reliable methods are important, in…
Surface molecules, distributed in diverse patterns and clusters on cell membranes, influence vital functions of living cells. It is therefore important to understand their molecular surface organisation under different physiological and…
The surface brightness fluctuations (SBF) method is a statistical method applied on image pixels in different bands. This contribution aims to distinguish between the observational (statistical) method and the theoretical (probabilistic)…
The free energetics of water density fluctuations near a surface, and the rare low-density fluctuations in particular, serve as reliable indicators of surface hydrophobicity; the easier it is to displace the interfacial waters, the more…
The Surface Brightness Fluctuations (SBF) Method for distance determinations of elliptical galaxies is been modeled in order to investigate the effect of the Point Spread Function (PSF). We developed a method to simulate observations of SBF…
Methods of determining surface diffusion coefficients of molecules from signal fluctuations of a locally fixed probe are revisited and refined. Particular emphasis is put on the influence of the molecule's extent. In addition to the…
Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…
The goal of this work is to scrutinise the surface brightness fluctuation (SBF) calculation methodology. We analysed the SBF derivation procedure, measured the accuracy of the fitted SBF under controlled conditions, retrieved the…
Recent applications have proved that the Surface Brightness Fluctuations (SBF) technique is a reliable distance indicator in a wide range of distances, and a promising tool to analyze the physical and chemical properties of unresolved…
Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…
Dwarf galaxies are important tracers of small-scale cosmological structure, yet much of our knowledge about these systems comes from the limited sample of dwarf galaxies within the Local Group. To make a comprehensive inventory of dwarf…
Recent experimental results have shown that active enzymes can diffuse faster when they are in the presence of their substrates. Fluorescence correlation spectroscopy (FCS), which relies on analyzing the fluctuations in fluorescence…
Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…
We introduce a novel framework for upsampled Point Spread Function (PSF) modeling using pixel-level Bayesian inference. Accurate PSF characterization is critical for precision measurements in many fields including: weak lensing, astrometry,…
We present a new method that combines fluorescence correlation spectroscopy (FCS) on the microsecond time scale with fluorescence antibunching measurements on the nanosecond time scale for measuring photophysical rate constants of…
We introduce a new method to determine the relative contributions of different types of stars to the integrated light of nearby early-type galaxies. As is well known, the surface brightness of these galaxies shows pixel-to-pixel…
The potential of the Surface Brightness Fluctuations (SBF) method to determine the membership of dwarf elliptical galaxies (dEs) in nearby galaxy clusters is investigated. Extensive simulations for SBF measurements on dEs in the I-band for…
We present an experimental method allowing to quantify slip at the wall in viscous polymer fluids, based on the observation of the evolution under simple shear flow of a photobleached pattern within a fluorescent labeled polymer melt. This…