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Spatial Transcriptomics (ST) technologies provide biologists with rich insights into single-cell biology by preserving spatial context of cells. Building foundational models for ST can significantly enhance the analysis of vast and complex…

Genomics · Quantitative Biology 2025-07-24 Suyuan Zhao , Yizhen Luo , Ganbo Yang , Yan Zhong , Hao Zhou , Zaiqing Nie

Spatial transcriptomics (ST) has revolutionised transcriptomics analysis by preserving tissue architecture, allowing researchers to study gene expression in its native spatial context. However, despite its potential, ST still faces…

Quantitative Methods · Quantitative Biology 2025-05-19 Anthony Baptista , Rosamond Nuamah , Ciro Chiappini , Anita Grigoriadis

Understanding how cellular morphology, gene expression, and spatial context jointly shape tissue function is a central challenge in biology. Image-based spatial transcriptomics technologies now provide high-resolution measurements of cell…

Quantitative Methods · Quantitative Biology 2026-02-17 Zhenglun Kong , Mufan Qiu , John Boesen , Xiang Lin , Sukwon Yun , Tianlong Chen , Manolis Kellis , Marinka Zitnik

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

Spatial transcriptomics (ST) provides essential spatial context by mapping gene expression within tissue, enabling detailed study of cellular heterogeneity and tissue organization. However, aligning ST data with histology images poses…

Spatial transcriptomics data analysis integrates cellular transcriptional activity with spatial coordinates to identify spatial domains, infer cell-type dynamics, and characterize gene expression patterns within tissues. Despite recent…

Quantitative Methods · Quantitative Biology 2026-03-25 Sean Cottrell , Guo-Wei Wei , Longxiu Huang

Spatial Transcriptomics (ST) is a method that captures gene expression profiles aligned with spatial coordinates. The discrete spatial distribution and the super-high dimensional sequencing results make ST data challenging to be modeled…

Machine Learning · Computer Science 2025-05-08 Qingtian Zhu , Yumin Zheng , Yuling Sang , Yifan Zhan , Ziyan Zhu , Jun Ding , Yinqiang Zheng

Spatial transcriptomics provides an unprecedented perspective for deciphering tissue spatial heterogeneity. However, high-resolution spatial transcriptomic technology remains constrained by limited gene coverage, technical complexity, and…

Biomolecules · Quantitative Biology 2026-05-19 Xinlei Huang , Weihao Dai , Zijun Qin , Xin Yu , Di Wang , Yanran Liu , Lixin Cheng , Xubin Zheng

Single-cell RNA sequencing (scRNA-seq) allows transcriptional profiling, and cell-type annotation of individual cells. However, sample preparation in typical scRNA-seq experiments often homogenizes the samples, thus spatial locations of…

Applications · Statistics 2024-08-28 Junhao Zhu , Kevin Zhang , Dehan Kong , Zhaolei Zhang

Single-cell spatial transcriptomics (ST) offers a unique approach to measuring gene expression profiles and spatial cell locations simultaneously. However, most existing ST methods assume that cells in closer spatial proximity exhibit more…

Genomics · Quantitative Biology 2025-06-10 Xiongtao Xiao , Xiaofeng Chen , Feiyan Jiang , Songming Zhang , Wenming Cao , Cheng Tan , Zhangyang Gao , Zhongshan Li

Spatial transcriptomics (ST) captures gene expression within distinct regions (i.e., windows) of a tissue slide. Traditional supervised learning frameworks applied to model ST are constrained to predicting expression from slide image…

Computer Vision and Pattern Recognition · Computer Science 2024-01-29 Yan Yang , Md Zakir Hossain , Xuesong Li , Shafin Rahman , Eric Stone

Spatial transcriptomics (ST) is an emerging technology that enables researchers to investigate the molecular relationships underlying tissue morphology. However, acquiring ST data remains prohibitively expensive, and traditional fixed-grid…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Junchao Zhu , Ruining Deng , Junlin Guo , Tianyuan Yao , Chongyu Qu , Juming Xiong , Siqi Lu , Zhengyi Lu , Yanfan Zhu , Marilyn Lionts , Yuechen Yang , Yalin Zheng , Yu Wang , Shilin Zhao , Haichun Yang , Yuankai Huo

Spatial transcriptomics has the potential to transform our understanding of RNA expression in tissues. Classical array-based technologies produce multiple-cell-scale measurements requiring deconvolution to recover single cell information.…

Spatial omics assays allow for the molecular characterisation of cells in their spatial context. Notably, the two main technological streams, imaging-based and high-throughput sequencing-based, can give rise to very different data…

Quantitative Methods · Quantitative Biology 2025-06-26 Martin Emons , Samuel Gunz , Helena L. Crowell , Izaskun Mallona , Reinhard Furrer , Mark D. Robinson

Spatial omics has transformed our understanding of tissue architecture by preserving spatial context of gene expression patterns. Simultaneously, advances in imaging AI have enabled extraction of morphological features describing the…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Eduard Chelebian , Christophe Avenel , Carolina Wählby

Deep learning-based nuclei segmentation and classification in pathology images typically rely on large-scale pixel-level manual annotations, which are costly and difficult to obtain across diverse tissues and staining conditions. To address…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Kazuya Nishimura , Ryoma Bise , Haruka Hirose , Yasuhiro Kojima

Temporal sequences of satellite images constitute a highly valuable and abundant resource for analyzing regions of interest. However, the automatic acquisition of knowledge on a large scale is a challenging task due to different factors…

Computer Vision and Pattern Recognition · Computer Science 2024-02-15 Carlos Echegoyen , Aritz Pérez , Guzmán Santafé , Unai Pérez-Goya , María Dolores Ugarte

Understanding the intricate cellular environment within biological tissues is crucial for uncovering insights into complex biological functions. While single-cell RNA sequencing has significantly enhanced our understanding of cellular…

Image and Video Processing · Electrical Eng. & Systems 2024-11-06 Bingjun Li , Mostafa Karami , Masum Shah Junayed , Sheida Nabavi

Developmental transcriptional networks in plants and animals operate in both space and time. To understand these transcriptional networks it is essential to obtain whole-genome expression data at high spatiotemporal resolution. Substantial…

Genomics · Quantitative Biology 2009-03-25 Dustin A. Cartwright , Siobhan M. Brady , David A. Orlando , Bernd Sturmfels , Philip N. Benfey