Related papers: A common framework for single-molecule localizatio…
Spatial Fluorescence Cross Correlation Spectroscopy is a rarely investigated version of Fluorescence Correlation Spectroscopy, in which the fluorescence signals from different observation volumes are cross-correlated. In the reported…
We suggest an approach to detect the conformation of single molecule by using the photon counting statistics. The generalized Smoluchoswki equation is employed to describe the dynamical process of conformational change of single molecule.…
Visible light positioning (VLP) technology is a promising technique as it can provide high accuracy positioning based on the existing lighting infrastructure. However, existing approaches often require dense lighting distributions.…
We describe a fast closed-loop optimization wavefront shaping system able to focus light through dynamic scattering media. A MEMS-based spatial light modulator (SLM), a fast photodetector and FPGA electronics are combined to implement a…
Super-resolution microscopy is crucial for imaging sub-wavelength biological structures. However, most techniques rely on nonlinear saturation or stochastic switching of emitters, limiting imaging speed and increasing phototoxicity. Here,…
A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…
Visual inspection of solar modules is an important monitoring facility in photovoltaic power plants. Since a single measurement of fast CMOS sensors is limited in spatial resolution and often not sufficient to reliably detect small defects,…
Few-shot learning (FSL) aims to learn novel visual categories from very few samples, which is a challenging problem in real-world applications. Many methods of few-shot classification work well on general images to learn global…
Object detection involves two sub-tasks, i.e. localizing objects in an image and classifying them into various categories. For existing CNN-based detectors, we notice the widespread divergence between localization and classification, which…
Over the last decade, single-molecule optical microscopy has become the gold-standard approach to decipher complex molecular processes in cellular environments. [1-3] Single-molecule fluorescence microscopy has several advantages such as…
This paper adapts a popular image quality measure called structural similarity for high precision registration based tracking while also introducing a simpler and faster variant of the same. Further, these are evaluated comprehensively…
Single fluorescent molecules, behaving as ideal electric dipole emitters, are powerful nanoscopic probes of complex optical fields. Here, this property is exploited to precisely map the polarization and vectorial structure of tightly…
We present a method that can simultaneously locate positions of overlapped multi-emitters at the theoretical-limit precision. We derive a set of simple equations whose solution gives the maximum likelihood estimator of multi-emitter…
Localization microscopy is an imaging technique in which the positions of individual nanoscale point emitters (e.g. fluorescent molecules) are determined at high precision from their images. This is the key ingredient in…
We report a method using scanning tunnelling microscope single molecular break junction to simultaneously measure and correlate the single-molecule thermopower and electrical conductance. In contrast to previously reported approaches, it…
The use of photo-activated fluorescent molecules to create long sequences of low emitter-density diffraction-limited images enables high-precision emitter localization, but at the cost of low temporal resolution. We suggest combining…
The microscopic orientation and position of photoactive molecules is crucial to the operation of optoelectronic devices such as OLEDs and solar cells. Here, we introduce a shape-persistent macrocyclic molecule as an excellent fluorescent…
Point Spread Function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline…
SPAD cameras offer single photon detection sensitivity, high frame rates and zero readout noise. They are a core technology for widefield FLIM, but have further potential in ultra-fast imaging applications. However, in practice sensitivity…
The on-demand tailoring of light's spatial shape is of great relevance in a wide variety of research areas. Computer-controlled devices, such as Spatial Light Modulators (SLMs) or Digital Micromirror Devices (DMDs), offer a very accurate,…